Detection of circulating free and immune-complexed antigen in pulmonary tuberculosis using cocktail of antibodies to mycobacterium tuberculosis excretory secretory antigens by peroxidase enzyme immunoassay

Indian Journal of Tuberculosis

Volumn 57, Issue 2, 2010, Pages 67-74

  Majumdar, Anindita ^a   Kamble, Pranita D ^a   Harinath, B C ^b  

^a Department of Biochemistry ^*

^b MAHATMA GANDHI INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Mycobacterial ES cocktail antigen; Peroxidase ELISA; Pulmonary tuberculosis

Indexed keywords

BACTERIUM ANTIBODY; MYCOBACTERIUM ANTIGEN; TUBERCULOSTATIC AGENT;

ACID FAST BACTERIUM; ANTIGEN DETECTION; ARTICLE; CLINICAL ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; INTERMETHOD COMPARISON; LUNG TUBERCULOSIS; MASS SCREENING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PROCESS DEVELOPMENT; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS;

ANTIBODIES, BACTERIAL; ANTIGEN-ANTIBODY COMPLEX; ANTIGENS, BACTERIAL; CASE-CONTROL STUDIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; PEROXIDASE; TUBERCULOSIS, PULMONARY;

EID: 78649565955     PISSN: 00195707     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. World health organization. Global tuberculosis control - a short update to the 2009 report. P- 4-5. Cited at http://www.who.int/tb/publications/global_report/2009/update/tbu_9.pdf
2. Frieden T R, Sterling T R, Munsiff S S, Watt C J, Dye C. Tuberculosis. Lancet 2003; 362: 887-899.
3. Grzybowski S, Barnett G D, Styblo K. Contacts of cases of active pulmonary tuberculosis. Bull Int Union Tuberc 1975; 50: 90-106
4. Kim T C, Blackman R S, Heatwole K M, Kim T, Rochester D F. Acid-fast bacilli in sputum smears of patients with pulmonary tuberculosis. Prevalence and significance of negative smears pretreatment and positive smears posttreatment. Am Rev Respir Dis 1984; 129: 264-268
5. Millen SJ, Uys PW, Hargrove J, van Helden PD, Williams BG. The Effect of Diagnostic Delays on the Drop-Out Rate and the Total Delay to Diagnosis of Tuberculosis. PLoS ONE. 2008 Apr 9;3(4):e1933
6. Storla DG, Yimer S, Bjune GA: A systematic review of delay in the diagnosis and treatment of tuberculosis. BMC Publ Heal 2008; 8: 15 (http://www.biomedcentral.com/1471-2458/8/15
7. Update: Fatal and severe liver injuries associated with rifampin and pyrazinamide for latent tuberculosis infection, and revisions in American Thoracic Society/CDC recommendations-United States, 2001. MMWR 2001; 50: 733-735
8. Yee Y C, Gough A, Kumarasinghe G, Lim T K. The pattern of utilisation and accuracy of a commercial nucleic acid amplification test for the rapid diagnosis of Mycobacterium tuberculosis in routine clinical practice. Singapore Med J 2002; 43: 415-420
9. Banerjee S, Gupta S, Shende N, Kumar S, Harinath B C. Serodiagnosis of tuberculosis using two ELISA systems. Ind J Clin Biochem 2003; 18: 48-53
10. Banerjee S, Gupta S, Kumar S, Shrikhande A V, Reddy MVR, Harinath BC. Seroreactivityo f 3l kDa and 4lkDa mycobacteriasl ecretoryp roteinsi solatedf rom culture filtrate in extra pulmonary tuberculosis. Indian J Pathol Microbiol 2003: 46: 261-264
11. Gupta S, Shende N, Kumar S, Hadnath BC. Antibody response to M.tb.H37Ra excretory-secretory ES-43 and ES-31 antigens at different stages of pulmonary tuberculosis. Biomed Res 2004; 15: 76-79
12. Bhatia AS, Gupta S, Shende N, Kumar S, Harinath BC. Serodiagnosis of Childhood Tuberculosis by ELISA. Ind J Pediatr 2005; 72(5): 383-387.
13. Lodam AN, Reddy MVR, Narang P, Gupta OP, Harinath BC. Fractionation analysis and diagnostic utility of Mycobacterium tuberculosis H37Ra excretory secretory antigen in pulmonary tuberculosis. Indian J Biochem Biophys 1996; 33: 67-71.
14. Gupta S, Shende N, Kumar S, Harinath BC. Detection of antibodies to a cocktail of mycobacterial excretory secretory antigens in tuberculosis by ELISA and Immunoblotting. Curr Sci 2005; 88: 1825-1827.
15. Harinath B C, Kumar S, Roy S S, Hirudkar S, Upadhye V, Shende N. A coctail of affinity purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43 and EST-6 for detecting the presence of Mycobacterium tuberculosis. Diag Microbio Inf Dis 2006; 55: 65-68.
16. Majumdar A, Upadhye V, Harinath BC. A Prospective Study of Inhouse Developed SEVA TB ELISA Using Cocktail of Antigens and their Immunoglobulin in the Diagnosis of Tuberculosis Suspected Patients in a Tertiary Hospital Located in Rural Area. Biomedical Research 2009; 20(1); 56-63.
17. Majumdar A, Upadhye V, Harinath BC. Peroxidase enzyme immunoassay for circulating SEVA TB ES-31 antigen in pulmonary tuberculosis sera. Biomedical research 2008; 19(3): 201-206.
18. Nair ER, Banerjee S, Kumar S, Reddy MVR, Harinath BC. Isolation of Mycobacterium tuberculosis 31 kDa antigen protein of diagnostic interest from culture filtrate using anti ES-31 antibody by affinity chromatography. Indian J Clin Biochem 2001; 16: 132-135.
19. Saha-Roy S, Shende N, Kumar S, Harinath BC. Effectivity of crude verss purified mycobacterial secretory protein as immunogen for optimum antibody production. Ind J Exp Biol 2005; 43: 1196-1198.
20. Upadhye V, Shende N, Kumar S, Harinath BC. Detec-tion of antibody and antigen in extrapulmonry tubercu-losis patients' sera using a cocktail of mycobacterial excretory secretory antigens and their antibodies. Biomed Res 2007; 18: 161-166.
21. Sauar NJ, Foulkes JA, O'Neil PM. A comparison of alkaline phosphatase, beta galactosidase, penicillinase and Peroxidase used as labels for progesterone determination in milk by heterogenous microtitre plate. Enzyme immunoassay. J Steroid Biochm 1989; 33: 423-426.