A microfluidic device for continuous white blood cell separation and lysis from whole blood

Artificial Organs

Volumn 34, Issue 11, 2010, Pages 996-1002

  Kim, Myounggon ^b   Mo Jung, Seung ^b   Lee, Kyeong Hwan ^c   Jun Kang, Yang ^b   Yang, Sung ^a,b,c  

^a Department of Nanobio Materials and Electronics ^*  (South Korea)

^b School of Mechatronics   (South Korea)

^c Gwangju Institute of Science and Technology (GIST)   (South Korea)

Author keywords

Blood cell lysis; Blood cell separation; DNA concentration; DNA purity; Microfluidic device; White blood cell recovery efficiency

Indexed keywords

BIFURCATION (MATHEMATICS); COMPUTATIONAL FLUID DYNAMICS; DNA; EFFICIENCY; FLUIDIC DEVICES; MICROFLUIDICS; MOLECULAR BIOLOGY; SEPARATION; SPECTROPHOTOMETRY; VISCOSITY;

BLOOD CELL LYSE; BLOOD CELL SEPARATION; BLOOD CELLS; CELL LYSIS; CELLS RECOVERY; DNA CONCENTRATION; DNA PURITY; MICROFLUIDICS DEVICES; RECOVERY EFFICIENCY; WHITE BLOOD CELL RECOVERY EFFICIENCY; WHITE BLOOD CELLS;

BLOOD;

DNA;

ARTICLE; BLOOD VISCOSITY; CELL SEPARATION; COMPUTATIONAL FLUID DYNAMICS; CYTOLYSIS; DNA PURIFICATION; ERYTHROCYTE; FLOW RATE; LEUKOCYTE; MICROFLUIDIC ANALYSIS; PRIORITY JOURNAL; SIMULATION; SPECTROPHOTOMETRY;

EID: 78649563104     PISSN: 0160564X     EISSN: 15251594     Source Type: Journal    
DOI: 10.1111/j.1525-1594.2010.01114.x     Document Type: Article

References (15)

1. Silverthorn DU. Human Physiology, 4th Edition. San Francisco, CA: Pearson Prentice, 2007.
2. Lie-Injo LE, Balasegaram M, Lopez CG, Herrera AR. Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma. DNA 1983;2:301-8.
3. Fitzgerald LM, Yin HZ, Kiessling AA. PCR amplification of HIV and cellular DNA sequences in formaldehyde-fixed, immunoreactive white blood cells. Biotechniques 1993;15:128-33.
4. Choi S, Song S, Choi C, Park JK. Continuous blood cell separation by hydrophoretic filtration. Lab Chip 2007;7:1532-8.
5. Choi S, Park JK. Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array. Lab Chip 2005;5:1161-7.
6. Pamme N, Wilhelm C. Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis. Lab Chip 2006;6:974-80.
7. Petersson F, Nilsson A, Jonsson H, Laurell T. Carrier medium exchange through ultrasonic particle switching in microfluidic channels. Anal Chem 2005;77:1216-21.
8. Carlo DD, Jeong KH, Lee LP. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation. Lab Chip 2003;3:287-91.
9. Lu KY, Wo AM, Lo YJ, Chen KC, Lin CM, Yang CR. Three dimensional electrode array for cell lysis via electroporation. Bioses Bioelectron 2006;22:568-74.
10. Sethu P, Anahtar M, Moldawer LL, Tompkins RG, Toner M. Continuous flow microfluidic device for rapid erythrocyte lysis. Anal Chem 2004;76:6247-53.
11. Lee CY, L0ee GB, Lin JL, Huang FC, Liao CS. Integrated microfluidic systems for cell lysis mixing/pumping and DNA amplification. J Micromech Microeng 2005;15:1215-23.
12. Yang S, Undar A, Zahn JD. A microfluidic device for continuous, real time blood plasma separation. Lab Chip 2006;6:871-80.
13. Han Z, Tang X, Zheng B. A PDMS viscometer for microliter Newtonian fluid. J Micromech Microeng 2007;17:1828-34.
14. Fung YC. Biomechanics: Mechanical Properties of Living Tissues, 2nd Edition. New York: Springer, 1993.
15. Glasel JA. Validity of nucleic acid purities monitored by A260/A280 absorbance ratios. Biotechniques 1995;18:62-3.