Fixation and Embedding of Cells and Tissues for Trnasmission Electron Microscopy

Cell Biology, Four-Volume Set

Volumn 3, Issue , 2006, Pages 221-231

  Maunsbach, Arvid B ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 78649556255     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50150-7     Document Type: Chapter

References (12)

1. Bohman S.-O., Maunsbach A.B. Effects on tissue fine structure of variations in colloid osmotic pressure of glutaraldehyde fixatives. J. Ultrastruct. Res. 1970, 30:195-208.
2. Carlemalm E., Villiger W., Hobot J.A., Acetarin J.-D., Kellenberger E. Low temperature embedding with Lowicryl resins: Two new formulations and some applications. J. Microsc. 1985, 140:55-63.
3. Glauert A.M., Lewis P.R. Biological specimen preparation for transmission electron microscopy. Practical Methods in Electron Microscopy 1998, Vol. 17. Portland Press, London. A.M. Glauert (Ed.).
4. Griffiths G. Fine Structure Immunocytochemistry 1993, Springer-Verlag, Berlin.
5. Karnovsky M.J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 1965, 27:137A.
6. Luft J.H. Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 1961, 9:409-414.
7. Maunsbach A.B. The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives. J. Ultrastruct. Res. 1966, 15:242-282.
8. Maunsbach A.B. The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. II. Effects of varying osmolality, ionic strength, buffer systems and fixative concentration of glutaraldehyde solutions. J. Ultrastruct. Res. 1996, 15:283-309.
9. Maunsbach A.B., Afzelius B.A. Biomedical Electron Microscopy. Illustrated Methods and Interpretations 1998, Academic Press, Sand Diego.
10. Mollenhauer H.H. Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy. Microsc. Res. Technique 1993, 26:496-512.
11. Ringo D.L., Brennan E.F., Cota-Robles E.H. Epoxy resins are mutagenic: Implications for electron microscopists. J. Ultrastruct. Res. 1982, 80:280-287.
12. Sabatini D.D., Bensch K., Barnett R.J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 1963, 17:19-58.