Characterisation of pdus, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Parsons, Joshua B ^a   Lawrence, Andrew D ^a   Mclean, Kirsty J ^b   Munro, Andrew W ^b   Rigby, Stephen E J ^b   Warren, Martin J ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; COBALAMIN; OXIDOREDUCTASE; OXYGEN; PROTEIN PDUS; PROTEIN PDUT; QUERCETIN; UNCLASSIFIED DRUG; AQUACOBALAMIN REDUCTASE; CORRIN; CORRINOID; FLAVOPROTEIN; IRON SULFUR PROTEIN; PROPYLENE GLYCOL; RECOMBINANT PROTEIN; RIBOFLAVIN DERIVATIVE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL METABOLISM; CITROBACTER FREUNDII; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; ELECTRON SPIN RESONANCE; GENETICS; METABOLISM; MUTATION; OXIDATION REDUCTION REACTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; ULTRAVIOLET SPECTROPHOTOMETRY;

BACTERIA (MICROORGANISMS); CITROBACTER FREUNDII;

BACTERIAL PROTEINS; CITROBACTER FREUNDII; CORRINOIDS; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FLAVINS; FLAVOPROTEINS; IRON-SULFUR PROTEINS; MUTATION; NADH, NADPH OXIDOREDUCTASES; OXIDATION-REDUCTION; OXIDOREDUCTASES; PROPYLENE GLYCOL; PROTEIN BINDING; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 78649494879     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0014009     Document Type: Article

References (26)

1. Banerjee R, Ragsdale SW (2003) The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes. Annu Rev Biochem 72: 209-247.
2. Roth JR, Lawrence JG, Bobik TA (1996) Cobalamin (coenzyme B12): synthesis and biological significance. Annu Rev Microbiol 50: 137-181.
3. Walker GA, Murphy S, Huennekens FM (1969) Enzymatic conversion of vitamin B 12a to adenosyl-B 12: evidence for the existence of two separate reducing systems. Arch Biochem Biophys 134: 95-102.
4. Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002) The biosynthesis of adenosylcobalamin (vitamin B12). Nat Prod Rep 19: 390-412.
5. Blanche F, Maton L, Debussche L, Thibaut D (1992) Purification and characterization of Cob(II)yrinic acid a,c-diamide reductase from Pseudomonas denitrificans. J Bacteriol 174: 7452-7454.
6. Lawrence AD, Deery E, McLean KJ, Munro AW, Pickersgill RW, et al. (2008) Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis. J Biol Chem 283: 10813-10821.
7. Lexa D, Saveant JM, Zickler J (1977) Electrochemistry of vitamin B12. 2. Redox and acid-base equilibria in the B12a/B12r system. J Am Chem Soc 99: 2786-2790.
8. Fonseca MV, Escalante-Semerena JC (2000) Reduction of Cob(III)alamin to Cob(II)alamin in Salmonella enterica serovar typhimurium LT2. J Bacteriol 182: 4304-4309.
9. Fonseca MV, Escalante-Semerena JC (2001) An in vitro reducing system for the enzymic conversion of cobalamin to adenosylcobalamin. J Biol Chem 276: 32101-32108.
10. Bobik TA, Havemann GD, Busch RJ, Williams DS, Aldrich HC (1999) The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation. J Bacteriol 181: 5967-5975.
11. Bobik TA, Xu Y, Jeter RM, Otto KE, Roth JR (1997) Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase. J Bacteriol 179: 6633-6639.
12. Sampson EM, Johnson CL, Bobik TA (2005) Biochemical evidence that the pduS gene encodes a bifunctional cobalamin reductase. Microbiology 151: 1169-1177.
13. Toraya T (2000) Radical catalysis of B12 enzymes: structure, mechanism, inactivation, and reactivation of diol and glycerol dehydratases. Cell Mol Life Sci 57: 106-127.
14. Brinsmade SR, Paldon T, Escalante-Semerena JC (2005) Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome. J Bacteriol 187: 8039-8046.
15. Cheng S, Liu Y, Crowley CS, Yeates TO, Bobik TA (2008) Bacterial microcompartments: their properties and paradoxes. Bioessays 30: 1084-1095.
16. Havemann GD, Bobik TA (2003) Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar Typhimurium LT2. J Bacteriol 185: 5086-5095.
17. Yeates TO, Kerfeld CA, Heinhorst S, Cannon GC, Shively JM (2008) Proteinbased organelles in bacteria: carboxysomes and related microcompartments. Nat Rev Microbiol 6: 681-691.
18. Mera PE, Escalante-Semerena JC (2010) Dihydroflavin-driven adenosylation of 4-coordinate Co(II) corrinoids: are cobalamin reductases enzymes or electron transfer proteins? J Biol Chem 285: 2911-2917.
19. Parsons JB, Dinesh SD, Deery E, Leech HK, Brindley AA, et al. (2008) Biochemical and structural insights into bacterial organelle form and biogenesis. J Biol Chem 283: 14366-14375.
20. Emptage MH, Dreyers JL, Kennedy MC, Beinert H (1983) Optical and EPR characterization of different species of active and inactive aconitase. J Biol Chem 258: 11106-11111.
21. Mera PE, St Maurice M, Rayment I, Escalante-Semerena JC (2009) Residue Phe112 of the human-type corrinoid adenosyltransferase (PduO) enzyme of Lactobacillus reuteri is critical to the formation of the four-coordinate Co(II) corrinoid substrate and to the activity of the enzyme. Biochemistry 48: 3138-3145.
22. St Maurice M, Mera P, Park K, Brunold TC, Escalante-Semerena JC, et al. (2008) Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate. Biochemistry 47: 5755-5766.
23. Stich TA, Buan NR, Escalante-Semerena JC, Brunold TC (2005) Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J Am Chem Soc 127: 8710-8719.
24. Cheng S, Bobik TA Characterization of the PduS cobalamin reductase of Salmonella enterica and its role in the Pdu microcompartment. J Bacteriol 192: 5071-5080.
25. Munro AW, Noble MA, Robledo L, Daff SN, Chapman SK (2001) Determination of the redox properties of human NADPH-cytochrome P450 reductase. Biochemistry 40: 1956-1963.
26. Dutton PL (1978) Redox potentiometry: determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems. Methods Enzymol 54: 411-435.