Obtaining of CD34+ cells from healthy blood donors: Development of a rapid and efficient procedure using leukoreduction filters

Transfusion

Volumn 50, Issue 10, 2010, Pages 2152-2157

  Peytour, Yann ^a,b,c   Guitart, Amélie ^a,b,c   Villacreces, Arnaud ^a,b,c   Chevaleyre, Jean ^a,b,c   Lacombe, Francis ^a,b,c   Ivanovic, Zoran ^a,b,c   Praloran, Vincent ^a,b,c  

^a CNRS Bordeaux Segalen University   (France)

^b ETABLISSEMENT FRANÇAIS DU SANG   (France)

^c BORDEAUX UNIVERSITY HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLOOD DONOR; BLOOD FILTER; CD34 SELECTION; CENTRIFUGATION; HUMAN; HUMAN CELL; LEUKOCYTE; LEUKOREDUCTION FILTER; MONONUCLEAR CELL; NORMAL HUMAN;

ANTIGENS, CD34; BLOOD DONORS; CELL DIFFERENTIATION; CELL SEPARATION; FLOW CYTOMETRY; HUMANS; LEUKOCYTE REDUCTION PROCEDURES; LEUKOCYTES, MONONUCLEAR;

EID: 78649453692     PISSN: 00411132     EISSN: 15372995     Source Type: Journal    
DOI: 10.1111/j.1537-2995.2010.02683.x     Document Type: Article

References (12)

1. Herbein G, Sovalat H, Wunder E, Baerenzung M, Bachorz J, Lewandowski H, Schweitzer C, Herbein G, Schmitt C, Kirn A. Isolation and identification of two CD34+ cell subpopulations from normal human peripheral blood. Stem Cells 1994;12:187-97.
2. Ivanovic Z, Duchez P, Morgan DA, Hermitte F, Lafarge X, Chevaleyre J, Praloran V, Dazey B, Vezon G, Boiron JM. Whole-blood leuko-depletion filters as a source of CD 34+ progenitors potentially usable in cell therapy. Transfusion 2006;46:118-25.
3. Meyer TP, Zehnter I, Hofmann B, Zaisserer J, Burkhart J, Rapp S, Weinauer F, Schmitz J, Illert WE. Filter buffy coats (FBC): a source of peripheral blood leukocytes recovered from leukocyte depletion filters. J Immunol Methods 2005; 307:150-66.
4. Bruil A, Beugeling T, Feijen J, van Aken WG. The mechanisms of leukocyte removal by filtration. Transfus Med Rev 1995;9:145-66.
5. Dzik S. Leukodepletion blood filters: filter design and mechanisms of leukocyte removal. Transfus Med Rev 1993; 7:65-77.
6. Hynes RO. Integrins: versatility, modulation, and signalling in cell adhesion. Cell 1992;69:11-25.
7. Barbe L, Boval B, Wautier MP, Wautier JL. Human promyelocytic cell line: a convenient tool for studying the molecular basis of WBC filtration. Transfusion 2000;40: 1250-6.
8. Masse M, Naegelen C, Pellegrini N, Segier JM, Marpaux N, Beaujean F. Validation of a simple method to count very low white cell concentrations in filtered red cells or platelets. Transfusion 1992;32:565-71.
9. Dzik S, Aubuchon J, Jeffries L, Kleinman S, Manno C, Murphy MF, Popovsky MA, Sayers M, Silberstein LE, Slichter SJ, Vamvakas EC. Leukocyte reduction of blood components: public policy and new technology. Transfus Med Rev 2000;14:34-52.
10. Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z. Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells. Stem Cells 2006;24:65-73.
11. Ebner S, Neyer S, Hofer S, Nussbaumer W, Romani N, Heufler C. Generation of large numbers of human dendritic cells from whole blood passaged through leukocyte removal filters: an alternative to standard buffy coats. J Immunol Methods 2001;252:93-104.
12. Neron S, Dussault N, Racine C. Whole-blood leukoreduction filters are a source for cryopreserved cells for phenotypic and functional investigations on peripheral blood lymphocytes. Transfusion 2006;46: 537-44.