Lentiviral labeling reveals three germ layer differentiation potential of a single unrestricted somatic stem cell from human cord blood

Experimental Hematology

Volumn 38, Issue 11, 2010, Pages 1099-1104

  Wernet, Peter ^a   Trapp, Thorsten ^a   Zweigerdt, Robert ^a   Mann, Jin ^a   Trompeter, Hans Ingo ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA;

ARTICLE; CELL CLONE; CELL DIFFERENTIATION; CELL LINEAGE; CELL STRAIN HEK293; CELL STRUCTURE; CONTROLLED STUDY; ECTODERM; ENDODERM; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; LENTIVIRINAE; MESODERM; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEM CELL; UMBILICAL CORD BLOOD; UNRESTRICTED SOMATIC STEM CELL;

BETA-GALACTOSIDASE; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINEAGE; CLONE CELLS; ECTODERM; ENDODERM; FETAL BLOOD; GENE EXPRESSION PROFILING; GERM LAYERS; HEK293 CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; LAC OPERON; LENTIVIRUS; MESODERM; MICRORNAS; NEURONS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OSTEOBLASTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS; TRANSDUCTION, GENETIC;

EID: 78649343271     PISSN: 0301472X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exphem.2010.09.006     Document Type: Article

References (16)

1. Kogler G., Sensken S., Airey J.A., et al. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med 2004, 200:123-135.
2. Kogler G., Sensken S., Wernet P. Comparative generation and characterization of pluripotent unrestricted somatic stem cells with mesenchymal stem cells from human cord blood. Exp Hematol 2006, 34:1589-1595.
3. Kogler G., Radke T.F., Lefort A., et al. Cytokine production and hematopoiesis supporting activity of cord blood-derived unrestricted somatic stem cells. Exp Hematol 2005, 33:573-583.
4. Kim B.O., Tian H., Prasongsukarn K., et al. Cell transplantation improves ventricular function after a myocardial infarction: a preclinical study of human unrestricted somatic stem cells in a porcine model. Circulation 2005, 112:I96-104.
5. Sensken S., Waclawczyk S., Knaupp A.S., et al. In vitro differentiation of human cord blood-derived unrestricted somatic stem cells towards an endodermal pathway. Cytotherapy 2007, 9:362-378.
6. Trapp T., Kogler G., El-Khattouti A., et al. Hepatocyte growth factor/c-MET axis-mediated tropism of cord blood-derived unrestricted somatic stem cells for neuronal injury. J Biol Chem 2008, 283:32244-32253.
7. Greschat S., Schira J., Kury P., et al. Unrestricted somatic stem cells from human umbilical cord blood can be differentiated into neurons with a dopaminergic phenotype. Stem Cells Dev 2008, 17:221-232.
8. Kogler G., Wernet P. Pluripotent stem cells from umbilical cord blood. Stem cell transplantation-biology, processes, therapy 2006, 73-85. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. A.D. Ho, R. Hoffmann, E.D. Zanjani (Eds.).
9. Lu R., Markowetz F., Unwin R.D., et al. Systems-level dynamic analyses of fate change in murine embryonic stem cells. Nature 2009, 462:358-362.
10. Laufs S., Gentner B., Nagy K.Z., et al. Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells. Blood 2003, 101:2191-2198.
11. Chen C., Ridzon D.A., Broomer A.J., et al. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 2005, 33:e179.
12. Kluth S.M., Buchheiser A., Houben A.P., et al. DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood. Stem Cells Dev 2010 Aug 28, [Epub ahead of print].
13. Liedtke S., Buchheiser A., Bosch J., et al. The HOX code as a " biological fingerprint" to distinguish functionally distinct stem cell populations derived from cord blood. Stem Cell Res 2010, 5:40-50.
14. Zaibak F., Bello P., Kozlovski J., et al. Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres. BMC Biotechnol 2009, 9:101.
15. Kern S., Eichler H., Stoeve J., Kluter H., Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006, 24:1294-1301.
16. Guo G., Huss M., Tong G.Q., et al. Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell 2010, 18:675-685.