Nuclear localization of cationic solid lipid nanoparticles containing Protamine as transfection promoter

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 76, Issue 3, 2010, Pages 384-393

  Vighi, Eleonora ^a   Montanari, Monica ^a   Ruozi, Barbara ^a   Tosi, Giovanni ^a   Magli, Alessandro ^a   Leo, Eliana ^a  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

Confocal microscopy; Gene delivery; Propidium iodide; Protamine; Solid lipid nanoparticles

Indexed keywords

BENZALKONIUM CHLORIDE; ESTERQUAT 1; PLASMID DNA; PROTAMINE; SOLID LIPID NANOPARTICLE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; GENETIC TRANSFECTION; NONHUMAN; PARTICLE SIZE; ZETA POTENTIAL;

ANIMALS; CATIONS; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; COS CELLS; DNA; GENETIC VECTORS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIPIDS; NANOPARTICLES; PARTICLE SIZE; PLASMIDS; PROTAMINES; QUATERNARY AMMONIUM COMPOUNDS; TRANSFECTION;

EID: 78549263702     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2010.07.012     Document Type: Article

References (42)

1. Verma M. A tumultuous year for gene therapy. Mol. Ther. 2000, 2:415-416.
2. Check E. Gene therapy: a tragic setback. Nature 2002, 420:116-118.
3. Smaglik P. Merck blocks 'safer' gene therapy trials. Nature 2000, 403:817.
4. Schmidt-Wolf G.D., Schmidt-Wolf I.G.H. Non-viral and hybrid vectors in human gene therapy: an update. Trends Mol. Med. 2003, 9:67-72.
5. Morille M., Passirani C., Vonarbourg A., Clavreul A., Benoit J.-P. Progress in developing cationic vectors for nonviral systemic gene therapy against cancer. Biomaterials 2008, 29:3477-3496.
6. Tabatt K., Sameti M., Olbrich C., Muller R.H., Lehr C.-M. Effect of cationic lipid and matrix lipid composition on solid lipid nanoparticles-mediated gene transfer. Eur. J. Pharm. Biopharm. 2004, 57:155-162.
7. Ferrer-Miralles N., Vasquez E., Villaverde A. Membrane-active peptides for nonviral gene therapy: making the safest easier. Trends Biotechnol. 2008, 26:267-275.
8. Somia N., Verma I.M. Gene therapy: trials and tribulations. Nat. Rev. Genet. 2000, 1:91-99.
9. Canine B.F., Wang Y., Hatefi A. Evaluation of the effect of vector architecture on DNA condensation and gene transfer efficiency. J. Control. Release 2008, 129:117-123.
10. Olbrich C., Bakowsky U., Lehr C.-M., Muller R.H., Kneuer C. Cationic solid-lipid nanoparticles can efficiently bind and transfect plasmid DNA. J. Control. Release 2001, 77:345-355.
11. Tabatt K., Kneuer C., Sameti M., Olbrich C., Muller R.H., Lehr C.-M., Bakowsky U. Transfection with different colloidal systems: comparison of solid lipid nanoparticles and liposomes. J. Control. Release 2004, 97:321-332.
12. Asasutjarit R., Lorenzen S.-I., Sirivichayakul S., Ruxrungtham K., Ruktanonchai U., Ritthidej G. Effect of solid lipid nanoparticles formulation composition on their size, zeta potential and potential for in vitro pHIS-HIV-hugag transfection. Pharm. Res. 2007, 24:1098-1107.
13. del Pozo-Rodriguez A., Delgado D., Solinis M.A., Gascon A.R., Pedraz J.L. Solid lipid nanoparticles: formulation factors affecting cell transfection capacity. Int. J. Pharm. 2007, 339:261-268.
14. Tsuchiya Y., Ishti T., Okahata Y., Sato T. Characterization of protamine as a transfection accelerator for gene delivery. J. Bioact. Compat. Polym. 2006, 21:519-537.
15. Braun R.E. Packaging paternal chromosomes with protamine. Nat. Genet. 2001, 28:10-12.
16. Liu J., Guo S., Li Z., Liu L., Gu J. Synthesis and characterization of stearyl protamine and investigation of their complexes with DNA for gene delivery. Coll. Surf. B: Biointer. 2009, 73:36-41.
17. Mayer G., Vogel V., Weyermann J., Lochmann D., van den Broek J.A., Tziatzios C., Haase W., Wouters D., Schubert U.S., Zimmer A., Kreuter J., Schubert D. Oligonucleotide-protamine-albumin nanoparticles: protamine sulfate causes drastic size reduction. J. Control. Release 2005, 106:181-187.
18. Gao X., Huang L. Potentiation of cationic liposome-mediated gene delivery by polycations. Biochemistry 1996, 35:1027-1036.
19. Sorgi F.L., Bhattacharya S., Huang L. Protamine sulfate enhances lipid-mediated gene transfer. Gene Ther. 1997, 4:961-968.
20. Weyermann J., Lochmann D., Georgens C., Zimmer A. Albumine-protamine-oligonucleotide nanoaprticles as a new antisense delivery system. Part 1: physicochemical characterization. Eur. J. Pharm. Biopharm. 2005, 59:431-438.
21. Weyermann J., Lochmann D., Georgens C., Zimmer A. Albumine-protamine-oligonucleotide nanoaprticles as a new antisense delivery system. Part 2: cellular uptake and effect. Eur. J. Pharm. Biopharm. 2005, 59:431-438.
22. Junghans M., Loitsch S.M., Steiniger S.C.J., Kreuter J., Zimmer A. Cationic lipid-protamine-DNA (LPD) complexes for delivery of antisense c-myc oligonucleotide. Eur. J. Pharm. Biopharm. 2005, 60:287-294.
23. Vighi E., Ruozi B., Montanari M., Battini R., Leo E. Re-dispersible cationic solid lipid nanoparticles (SLNs) freeze-dried without cryoprotectors: characterization and ability to bind the pEGFP-plasmid. Eur. J. Pharm. Biopharm. 2007, 67:320-328.
24. Mehnert W., Mäder K. Solid lipid nanoparticles: production, characterization and applications. Adv. Drug Deliv. Rev. 2001, 47:165-196.
25. Zhang Q., Yie G., Li Y., Yang Q., Nagai T. Studies on the cyclosporin A loaded stearic acid nanoparticles. Int. J. Pharm. 2000, 200:153-159.
26. Pedersen N., Hansen S., Heydenreich A.V., Kristensen H.G., Poulsen H.S. Solid lipid nanoparticles can effectively bind DNA, streptavidin and biotinylated ligands. Eur. J. Pharm. Biopharm. 2006, 62:155-162.
27. Vighi E., Ruozi B., Montanari M., Battini R., Leo E. pDNA condensation capacity and in vitro gene delivery properties of cationic solid lipid nanoparticles. Int. J. Pharm. 2010, 15:254-261.
28. Wrobel K., Claudio E., Segade F., Ramos S., Laze P.S. Measurement of cytotoxicity by propidium iodide staining of target cell DNA. Application to the quantification of murine TNF-α. J. Immunol. Methods 1996, 189:243-249.
29. Xu Z., Pilch D.S., Srinivasan A.R., Olson W.K., Geacintov N.E., Breslauer K.J. Modulation of nucleic acid structure by ligand binding: induction of a PDNA-RNA-PDNA hybrid triplex by DAPI intercalation. Bioorg. Med. Chem. 1997, 5:1137-1147.
30. del Pozo-Rodríguez A., Solinís M.A., Gascón A.R., Pedraz J.L. Short- and long-term stability study of lyophilized solid lipid nanoparticles for gene therapy. Eur. J. Pharm. Biopharm. 2009, 71:181-189.
31. Elouahabi A., Ruysschaert J.M. Formation and intracellular trafficking of lipoplexes and polyplexes. Mol. Ther. 2005, 11:336-347.
32. Han S.O., Mahato R.I., Sung Y.K., Kim S.W. Development of biomaterials for gene therapy. Mol. Ther. 2000, 2:302-317.
33. Godbey W.T., Wu K.K., Mikos A.G. Poly(ethylenimine) and its role in gene delivery. J. Control. Release 1999, 60:149-160.
34. Fischer D., Li Y., Ahlemeyer B., Krieglstein J., Kissel T. In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. Biomaterials 2003, 24:1121-1131.
35. Lv H., Zhang S., Wang B., Cui S., Yan J. Toxicity of cationic lipids and cationic polymers in gene delivery. J. Control. Release 2006, 114:100-109.
36. Guliyeva U., Onerv F., Ozsoy S., Haziroglu R. Chitosan microparticles containing plasmid DNA as potential oral gene delivery system. Eur. J. Pharm. Biopharm. 2006, 62:17-25.
37. Dass C.R., Contreras K.G., Dunstain D.E., M Choong P.F. Chitosan microparticles encapsulating PEDF plasmid demonstrate efficacy in an orthopic metastatic model of osteosarcoma. Biomaterials 2007, 28:3026-3033.
38. Ciftci K., Levy R.J. Enhanced plasmid DNA transfection with lysosomotropic agents in cultured fibroblasts. Int. J. Pharm. 2001, 218:81-92.
39. Ignatovich I.A., Dizhe E.B., Pavlotskaya A.V., Akifiev B.N., Burov S.V., Orlov S.V. Complexes of plasmid DNA with basic domain 47-57 of the HIV-1 Tat protein are transferred to mammalian cells by endocytosis-mediated pathways. J. Biol. Chem. 2003, 278:42625-42636.
40. Masuda T., Akita H., Harashima H. Evaluation of nuclear transfer and transcription of plasmid DNA condensed with Protamine. Mol. Ther. 2005, 11:S289-S290.
41. Vasquez E., Ferrer-Miralles N., Villaverde A. Peptide-assisted traffic engineering for nonviral gene therapy. Drug Discov. Today 2008, 13:1067-1074.
42. http://www.qiagen.com/products/plasmid/qiagenplasmidpurificationsystem/qiagenplasmidmaxikit.aspx.