Development of SYBR green-based real-time PCR and duplex nested PCR assays for quantitation and differential detection of species- or type-specific porcine Torque teno viruses

Journal of Virological Methods

Volumn 170, Issue 1-2, 2010, Pages 140-146

  Huang, Y W ^a   Dryman, B A ^a   Harrall, K K ^a   Vaughn, E M ^b   Roof, M B ^b   Meng, X J ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b BOEHRINGER INGELHEIM VETMEDICA INC   (United States)

Author keywords

Duplex nested PCR; Porcine Torque teno virus; Real time PCR; SYBR green; Torque teno virus

Indexed keywords

DYE; SYBR GREEN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DUPLEX NESTED POLYMERASE CHAIN REACTION; GENE AMPLIFICATION; GENE SEQUENCE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; TORQUE TENO VIRUS; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD; VIRUS TITRATION; VIRUS TYPING;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; DNA VIRUS INFECTIONS; DNA, VIRAL; MOLECULAR DIAGNOSTIC TECHNIQUES; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; STAINING AND LABELING; SWINE; SWINE DISEASES; TORQUE TENO VIRUS;

DNA VIRUSES; SUIDAE; SUS; TORQUE TENO VIRUS;

EID: 78249241205     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.09.018     Document Type: Article

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