Influence of the KDEL signal, DMSO and mannitol on the production of the recombinant antibody 14D9 by long-term Nicotiana tabacum cell suspension culture

Plant Cell, Tissue and Organ Culture

Volumn 103, Issue 3, 2010, Pages 307-314

  Lopez J ^a   Lencina, F ^a   Petruccelli, S ^b   Marconi, P ^a   Alvarez, M A ^a  

^a Fundacion Pablo Cassara   (Argentina)

^b FACULTAD DE CIENCIAS EXACTAS   (Argentina)

Author keywords

14D9; In vitro plant cell cultures; Molecular farming; Recombinant proteins

Indexed keywords

14D9; CATALYTIC ANTIBODIES; CELL DAMAGE; CULTURE MEDIUM; ENDOPLASMIC RETICULUM; IN-LINE; IN-VITRO; MOLECULAR FARMING; NICOTIANA TABACUM; OSMOTIC STRESS; PRODUCTION LEVEL; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SECRETORY PRODUCTS; TAGGED PRODUCTS; TRANSGENIC CELLS;

ANTIBODIES; DIMETHYL SULFOXIDE; GENE TRANSFER; ORGANIC SOLVENTS; PLANT CELL CULTURE; PLANTS (BOTANY);

CELL CULTURE;

NICOTIANA TABACUM;

EID: 78149500151     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-010-9780-y     Document Type: Article

References (25)

1. Alvarez MA, Nigra H, Giulietti AM (1993) Solasodine production by Solanum eleagnifolium Cav. in vitro cultures: Influence of plant growth regulators, age and inoculum size. Large-scale production. Nat Prod Lett 3: 9-19.
2. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
3. De Wilde C, De Neve M, De Ryke R, Bruyns A, De Jaeger G, van Montagu M, Depicker A, Engler G (1996) Intact antigen-binding MAK33 antibody and Fab fragment accumulate in intercellular spaces of Arabidopsis thaliana. Plant Sci 114: 233-241.
4. Doran PM (2006a) Loss of secreted antibody from transgenic plant tissue cultures due to surface adsorption. J Biotechnol 122: 39-54.
5. Doran PM (2006b) Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol 24: 426-432.
6. Hellwing S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 22: 1415-1422.
7. Henderson T, Henderson R, York J (1975) Effects of dimethyl sulfoxide on subunit proteins. Ann NY Acad Sci 243: 38-53.
8. Huang T-K, McDonald KA (2009) Bioreactor engineering for recombinant protein production in plant cell suspension cultures. Biochem Eng J 45: 168-184.
9. Kohli A, Twyman R, Abranches R, Wegel E, Stoger E, Christou P (2003) Transgene integration, organization and interaction in plants. Plant Mol Biol 52: 247-258.
10. Ma Julian K-C, Barros E, Bock Ralph CP, Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S, Sparrow P, Stoger E, Twyman RM (2005) Molecular farming for new drugs and vaccines. EMBO Rep 6: 593-599.
11. Martínez C, Giulietti AM, Petruccelli S, Alvarez MA (2005) Expression of the antibody 14D9 in Nicotiana tabacum hairy roots. Electron J Biotechnol 8: 170-176.
12. Mett V, Farrance CE, Green BJ, Yusibov V (2008) Plant as biofactories. Biologicals 36: 354-358.
13. Payne G, Bringi V, Prince C, Shuler M (1991) Quantifying growth and product synthesis: kinetics and stoichiometry. In: Michael S (ed) Plant cell and tissue culture in liquid systems. Hanser/Oxford University Press, Oxford, pp 47-70.
14. Pena LB, Pasquini LA, Tomaro ML, Gallego SM (2006) Proteolytic system in sunflower (Helianthusannuus L.) leaves under cadmium stress. Plant Sci 171: 531-537.
15. Petruccelli S, Otegui MS, Lareu F, Trandinhthanhlien O, Fitchette A-C, Circosta A, Rumbo M, Bardor M, Carcamo R, Gomord V, Beachy RN (2006) A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds. Plant Biotechnol J 4: 511-527.
16. Shabat D, Shulman H, Itzhaky H, Reymond JL, Keinan E (1998) Enantioselectivity versus kinetic resolution in antibody catalysis: formation of the (S) product despite preferential binding of the (R) intermediate. Chem Commun (Cambridge) 1998: 1759-1760.
17. Sharp JM, Doran PM (2001) Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol Prog 17: 979-992.
18. Soderquist R, Lee J (2005) Enhanced production of recombinant proteins from plant cells by the application of osmotic stress and protein stabilization. Plant Cell Rep 24: 127-132.
19. Tepfer M, Taylor IEP (1981) The interaction of divalent cations with pectic substances and their influence on acid-induced cell wall loosening. Can J Bot 59: 1522-1525.
20. van Handel E (1968) Direct microdetermination of sucrose. Anal Biochem 22: 280-283.
21. Vázquez SC, MacCormack W (2002) Effect of isolation temperature on the characteristics of extracellular proteases produced by Antarctic bacteria. Polar Res 21: 63-71.
22. Wahl MF, Gynheung A, Lee M (1995) Effect of dimethyl sulfoxide in heavy chain monoclonal antibody production from plant cell culture. Biotechnol Lett 17: 463-468.
23. Wimmer R, Olsson M, Petersen M, Hatti-Kaul R, Petersen S, Muller N (1997) Towards a molecular level understanding of protein stabilization: the interaction between lysozyme and sorbitol. J Biotechnol 55: 85-100.
24. Wongsamuth R, Doran PM (1997) Hairy roots as an expression system for production of antibodies. In: Doran PM (ed) Hairy roots: culture and application. Harwood Academic, Amsterdam, pp 89-97.
25. Zheng L, Baumann U, Reymond J (2003) Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm. J Biochem 133: 577-581.