A method for determining cell number in the undisturbed epithelium of the mouse lens

Molecular Vision

Volumn 16, Issue , 2010, Pages 2294-2300

  Bassnett, Steven ^a   Shi, Yanrong ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATARACT; CELL COUNT; CELL LABELING; CELL PROLIFERATION; COMPUTER ASSISTED DIAGNOSIS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EXPERIMENTAL MODEL; IMAGE QUALITY; IN SITU HYBRIDIZATION; LENS EPITHELIUM; LENS FIBER; LENS NUCLEUS; MOUSE; NONHUMAN; OPTIC TRACT; PRIORITY JOURNAL; RETINOTECTAL PROJECTION; SIGNAL NOISE RATIO; SURFACE PROPERTY; VISUAL FIELD;

ANIMALS; CELL COUNT; CELL NUCLEUS; EPITHELIAL CELLS; LENS, CRYSTALLINE; MICE; MICE, INBRED C57BL;

EID: 78149485475     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Pendergrass W, Penn P, Possin D, Wolf N. Accumulation of DNA, nuclear and mitochondrial debris, and ROS at sites of age-related cortical cataract in mice. Invest Ophthalmol Vis Sci 2005; 46:4661-70.
2. Pendergrass W, Zitnik G, Tsai R, Wolf N. X-ray induced cataract is preceded by LEC loss, and coincident with accumulation of cortical DNA, and ROS; similarities with age-related cataracts. Mol Vis 2010; 16:1496-513.
3. Abe T, Sato M, Kuboki J, Kano T, Tamai M. Lens epithelial changes and mutated gene expression in patients with myotonic dystrophy. Br J Ophthalmol 1999; 83:452-7.
4. Tkachov SI, Lautenschlager C, Ehrich D, Struck HG. Changes in the lens epithelium with respect to cataractogenesis: light microscopic and Scheimpflug densitometric analysis of the cataractous and the clear lens of diabetics and non-diabetics. Graefes Arch Clin Exp Ophthalmol 2006; 244:596-602.
5. Johar SR, Rawal UM, Jain NK, Vasavada AR. Sequential effects of ultraviolet radiation on the histomorphology, cell density and antioxidative status of the lens epithelium-an in vivo study. Photochem Photobiol 2003; 78:306-11.
6. Rafferty NS, Rafferty KA Jr. Cell population kinetics of the mouse lens epithelium. J Cell Physiol 1981; 107:309-15.
7. Von Sallmann L, Caravaggio L, Munoz C, Drungis A. Species differences in the radiosensitivity of the lens. Am J Ophthalmol 1957; 43:693-704.
8. Riley EF, Devi SK. Dynamics of cell populations in the rat lens epithelium. Exp Eye Res 1967; 6:383-92.
9. Wiley LA, Shui YB, Beebe DC. Visualizing lens epithelial cell proliferation in whole lenses. Mol Vis 2010; 16:1253-9.
10. Harding CV, Hughes WL, Bond VP, Schork P. Autoradiographic localization of tritiated thymidine in wholemount preparations of lens epithelium. Arch Ophthalmol 1960; 63:58-65.
11. Bassnett S. Three-dimensional reconstruction of cells in the living lens: the relationship between cell length and volume. Exp Eye Res 2005; 81:716-23.
12. von Sallmann L. Experimental studies on early lens changes after roentgen irradiation. III. Effect of X-irradiation on mitotic activity and nuclear fragmentation of lens epithelium in normal and cysteine-treated rabbits. Arch Ophthalmol 1952; 47:305-20