Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester

Applied Biochemistry and Biotechnology

Volumn 162, Issue 8, 2010, Pages 2387-2399

  Wang, Yi ^a   Zhao, Jian ^a   Xu, Jian He ^a   Fan, Li Qiang ^a   Li, Su Xia ^a   Zhao, Li Li ^a   Mao, Xiao Bo ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Biocatalytic resolution; Characterization; Enantioselectivity; Lipase; Purification; Serratia marcescens ECU1010; Soluble expression

Indexed keywords

BIOCATALYTIC RESOLUTION; BIOCHEMICAL PROPERTIES; BIPHASIC SYSTEMS; CHIRAL ESTERS; CHIRAL SYNTHONS; CONVERSION YIELD; CULTURE CONDITIONS; DILTIAZEM; ENANTIOSELECTIVE LIPASE; EXPRESSION VECTORS; FUSION PROTEINS; HIS-TAG; KINETIC RESOLUTION; LARGE-SCALE PRODUCTION; LAURATE; LIPASE ACTIVITY; LIPASE GENE; METHOXY; METHYL ESTERS; PH OPTIMA; PH RANGE; PHOSPHATE BUFFERS; PRACTICAL USE; PURIFICATION FACTORS; SERRATIA MARCESCENS; SHAKE FLASKS; SOLUBLE EXPRESSION;

AFFINITY CHROMATOGRAPHY; ENANTIOSELECTIVITY; ESCHERICHIA COLI; ESTERIFICATION; ESTERS; FATTY ACIDS; METAL IONS; NONIONIC SURFACTANTS; PURIFICATION; TOLUENE;

GENE EXPRESSION;

3 (4' METHOXYPHENYL)GLYCIDYL METHYL ESTER; BACTERIAL ENZYME; CALCIUM ION; COPPER ION; DILTIAZEM; ESTER; HYBRID PROTEIN; RECOMBINANT ENZYME; SURFACTANT; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYST; CHIRALITY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PH; PROTEIN EXPRESSION; SERRATIA MARCESCENS; TEMPERATURE;

BIOCATALYSIS; CLONING, MOLECULAR; ENZYME STABILITY; ESTERS; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; METALS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERRATIA MARCESCENS; SOIL MICROBIOLOGY; SOLUBILITY; STEREOISOMERISM; SUBSTRATE SPECIFICITY; SURFACE-ACTIVE AGENTS; TEMPERATURE;

ESCHERICHIA COLI; SERRATIA MARCESCENS;

EID: 78149471620     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-9011-3     Document Type: Article

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