Pushing the limits for amplifying BrdU-labeled DNA encoding 16S rRNA: DNA polymerase as the determining factor

Journal of Microbiological Methods

Volumn 83, Issue 3, 2010, Pages 312-316

  Roux Michollet, Dad D ^a   Schimel, Joshua P ^a   Holden, Patricia A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Active bacteria; BrdU labeled DNA; DNA polymerase; PCR troubleshooting; T RFLP

Indexed keywords

BROXURIDINE; DNA POLYMERASE; RNA 16S;

ARTICLE; BACTERIAL CELL; CELL LABELING; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA ISOLATION; ENZYME ACTIVITY; GENE SEQUENCE; MICROBIAL COMMUNITY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SOIL ANALYSIS;

BACTERIA; BROMODEOXYURIDINE; DNA, BACTERIAL; DNA-DIRECTED DNA POLYMERASE; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SOIL; SOIL MICROBIOLOGY; STAINING AND LABELING;

BACTERIA (MICROORGANISMS);

EID: 78149470892     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.09.016     Document Type: Article

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