Construction of a thermostable cell adhesion protein for reverse transfection

Journal of Biotechnology

Volumn 150, Issue 3, 2010, Pages 447-451

  Bae, JooYoun ^a   Goto, Sayaka ^a   Mie, Masayasu ^a   Kobatake, Eiry ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Cell adhesion; ECM (extracellular matrix); Gene transfection; RGD peptide; Thermal stability

Indexed keywords

ARG GLY ASPS; CELL-ADHESION PROTEINS; CELL-ADHESIVES; ECM (EXTRACELLULAR MATRIX); ELASTIN-DERIVED PEPTIDE; EXTRACELLULAR MATRICES; EXTRACELLULAR MATRIX PROTEIN; FUSION PROTEINS; GENE TRANSFECTION; MULTIPLE GENES; REVERSE TRANSFECTION; RGD PEPTIDE; THERMAL STABILITY; TRANSFECTION EFFICIENCY;

CELL ADHESION; GENES; PEPTIDES; THERMODYNAMIC STABILITY;

ADHESION;

ALANYLPROLYLGLYCYLVALYLGLYCYLVALINE; ARGINYLGLYCYLASPARTIC ACID; ELASTIN; FIBRONECTIN; HYBRID PROTEIN; PEPTIDE; PROLYLHISTIDYLSERYLARGINYLASPARAGININE; PROTEIN E12PSGR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ADHESION; GENETIC TRANSFECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMOSTABILITY;

ANIMALS; CELL ADHESION; CELL LINE; ELASTIN; ESCHERICHIA COLI; EXTRACELLULAR MATRIX PROTEINS; FIBRONECTINS; HOT TEMPERATURE; MICE; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 78149465560     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.09.960     Document Type: Article

References (13)

1. Aota S., Nomizu M., Yamada K.M. The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function. J. Biol. Chem. 1994, 269(40):24756-24761.
2. Dankers P.Y., Harmsen M.C., Brouwer L.A., van Luyn M.J., Meijer E.W. A modular and supramolecular approach to bioactive scaffolds for tissue engineering. Nat. Mater. 2005, 4(7):568-574.
3. Elloumi I., Kobayashi R., Funabashi H., Mie M., Kobatake E. Construction of epidermal growth factor fusion protein with cell adhesive activity. Biomaterials 2006, 27:3451-3458.
4. Erfle H., Simpson J.C., Bastiaens P.I., Pepperkok R. siRNA cell arrays for high-content screening microscopy. Biotechniques 2004, 37(3). pp. 454-458, 460, 462.
5. Kobatake E., Onoda K., Yanagida Y., Aizawa M. Design and gene engineering synthesis of an extremely thermostable protein with biological activity. Biomacromolecules 2000, 1(3):382-386.
6. Kobatake E., Onoda K., Yanagida Y., Haruyama T., Aizawa M. Design of a thermostable cell adhesion protein. Biotechnol. Techn. 1999, 13:23-27.
7. Main A.L., Harvey T.S., Baron M., Boyd J., Campbell I.D. The three-dimensional structure of the tenth type III module of fibronectin: an insight into RGD-mediated interactions. Cell 1992, 13, 71(4):671-678.
8. Mardilovich A., Craig J.A., McCammon M.Q., Garg A., Kokkoli E. Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized biomaterials. Langmuir 2006, 22(7):3259-3264.
9. Nakamura M., Mie M., Mihara H., Nakamura M., Kobatake E. Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells. Biomaterials 2008, 29:2977-2986.
10. Pierschbacher M.D., Ruoslahti E. Cell attachment activity of fibronectin can be duplicated by small synthetic fragment of the molecule. Nature 1984, 309:30-33.
11. Silva J.M., Mizuno H., Brady A., Lucito R., Hannon G.J. RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 2004, 101(17):6548-6552.
12. Yoshikawa T., Uchimura E., Kishi M., Funeriu D.P., Miyake M., Miyake J. Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown. J. Control. Release 2004, 96(2):227-232.
13. Ziauddin J., Sabatini D.M. Microarrays of cells expressing defined cDNAs. Nature 2001, 411(6833):107-110.