Evaluation of nitrate reductase assay for direct detection of drug resistance in Mycobacterium tuberculosis: Rapid and inexpensive method for low-resource settings

Indian Journal of Medical Microbiology

Volumn 28, Issue 4, 2010, Pages 363-365

  Gupta, M ^a   Shamma, ^a   Singh, N P ^a   Kaur, I R ^a  

^a UCMS AND GTB HOSPITAL   (India)

Author keywords

Drug susceptibility testing; M. tuberculosis; nitrate reductase assay

Indexed keywords

ETHAMBUTOL; ISONIAZID; NITRATE REDUCTASE; RIFAMPICIN; STREPTOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CULTURE MEDIUM; DIAGNOSTIC ACCURACY; DRUG SENSITIVITY; ENZYME ASSAY; EQUIPMENT; HUMAN; MULTIDRUG RESISTANCE; MYCOBACTERIUM TUBERCULOSIS; NITRATE REDUCTASE ASSAY; NONHUMAN; SENSITIVITY AND SPECIFICITY; SPUTUM SMEAR;

EID: 78149463004     PISSN: 02550857     EISSN: 19983646     Source Type: Journal    
DOI: 10.4103/0255-0857.71816     Document Type: Article

References (8)

1. World Health Organization. 2004. The W.H.O./IUATLD global project on anti-tuberculosis drug resistance surveillance. Anti-tuberculosis drug resistance in the world, report no. 3: WHO/HTM/TB/2005.349.WHO, Geneva, Switzerland.
2. Mishra B, Muralidharan S, Srinivasa H. Direct drug susceptibility testing of Mycobacterium tuberculosis to primary anti-tubercular drugs by nitrate reductase assay. Indian J Pathol Microbiol 2009;52:343-4.
3. Training Manual for Mycobacterium tuberculosis. Available from: http://www.tbcindia.org/pdfs/DST-DRStrainingmanualRNTCP.pdf [last accessed on 2009 Oct 1].
4. Musa HR, Ambroggi M, Souto A, Angeby KA. Drug susceptibility testing of Mycobacterium tuberculosis by a nitrate reductase assay applied directly on microscopypositive sputum samples. J Clin Microbiol 2005;43:3159-61. (Pubitemid 40981317)
5. Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, Mitchison DA, et al. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ 1969;41:21-43.
6. Syre H, Phyu S, Sandven P, Bjorvatn B, Grewal HM. Rapid colorimetric method for testing susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin in liquid cultures. J Clin Microbiol 2003;41:5173-7. (Pubitemid 37429932)
7. Lemus D, Montoro E, Martin EA, Portaels F, Palomino JC. Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: Simple and inexpensive method for low-resource laboratories. J Med Microbiol 2006;55:861-3. (Pubitemid 43974691)
8. Sethi S, Sharma S, Sharma SK, Meharwal SK, Jindal SK, Sharma M. Drug susceptibility of Mycobacterium tuberculosis to primary antitubercular drugs by nitrate reductase assay. Indian J Med Res 2004;120:468-71. (Pubitemid 40044917)