Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments

Analytical Biochemistry

Volumn 408, Issue 2, 2011, Pages 348-350

  Kinoshita Kikuta, Emiko ^a   Kinoshita, Eiji ^a   Harada, Naoko ^a   Koike, Tohru ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

1 ,4 ,7 ,10-TETRAAZACYCLODODECANE; BRCA1 GENE; BREAST CANCER; DNA FRAGMENT; GEL-BASED; MUTATION DETECTION; PCR PRIMERS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ZINC COMPOUNDS;

BRCA1 PROTEIN; DNA FRAGMENT; ZINC;

ARTICLE; CHEMICAL BINDING; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM;

EID: 78149412360     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.09.003     Document Type: Article

References (11)

1. Kinoshita-Kikuta E., Kinoshita E., Koike T. A novel procedure for simple and efficient genotyping of single nucleotide polymorphisms by using the Zn2+ -cyclen complex. Nucleic Acids Res. 2002, 30:e126.
2. Shionoya M., Kimura E., Shiro M. A new ternary zinc(II) complex with [12]aneN4 (=1,4,7,10-tetraazacyclododecane) and AZT (=3′-azido-3′-deoxythymidine). Highly selective recognition of thymine and its related nucleotides by a zinc(II) macrocyclic tetramine complex with novel complementary associations. J. Am. Chem. Soc. 1993, 115:6730-6737.
3. Kikuta E., Murata M., Katsube N., Koike T., Kimura E. Novel recognition of thymine base in double-stranded DNA by zinc(II) - macrocyclic tetramine complexes appended with aromatic groups. J. Am. Chem. Soc. 1999, 121:5426-5436.
4. Kinoshita E., Kinoshita-Kikuta E., Koike T. A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions. Anal. Biochem. 2005, 337:154-160.
5. Kinoshita E., Kinoshita-Kikuta E., Kojima H., Nakano Y., Chayama K., Koike T. Reliable and cost-effective screening of inherited heterozygosity by Zn2+cyclen polyacrylamide gel electrophoresis. Clin. Chem. (Washington, DC, US.) 2005, 51:2195-2197.
6. Kinoshita E., Kinoshita-Kikuta E., Nakashima H., Koike T. Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes. Clin. Biochem. 2010, 43:302-306.
7. Kinoshita E., Kinoshita-Kikuta E., Yoshimoto M., Koike T. Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn2+ cyclen polyacrylamide gel electrophoresis. Anal. Biochem. 2008, 380:122-127.
8. Peelen T., van Vliet M., Petrij-Bosch A., Mieremet R., Szabo C., van den Ouweland A.M.W., Hogervorst F., Brohet R., Ligtenberg M.J.L., Teugels E., van der Luijt R., van der Hout A.H., Gille J.J.P., Pals G., Jedema I., Olmer R., van Leeuwen I., Newman B., Plandsoen M., van der Est M., Brink G., Hageman S., Arts P.J.W., Bakker M.M., Willems H.W., van der Looij E., Neyns B., Bonduelle M., Jansen R., Oosterwijk J.C., Sijmons R., Smeets H.J.M., van Asperen C.J., Meijers-Heijboer H., Klijn J.G.M., de Greve J., King M.-C., Menko F.H., Brunner H.G., Halley D., van Ommen G.-J.B., Vasen H.F.A., Cornelisse C.J., van't Veer L.J., de Knijff P., Bakker E., Devilee P. A high proportion of novel mutations in BRCA1 with strong founder effects among Dutch and Belgian hereditary breast and ovarian cancer families. Am. J. Hum. Genet. 1997, 60:1041-1049.
9. Castilla L.H., Couch F.J., Erdos M.R., Hoskins K.F., Calzone K., Garber J.E., Boyd J., Lubin M.B., Deshano M.L., Brody L.C., Collins F.S., Weber B.L. Mutations in the BRCA1 gene in families with early-onset breast and ovarian cancer. Nat. Genet. 1994, 8:387-391.
10. Futreal P.A., Liu Q., Shattuck-Eidens D., Cochran C., Harshman K., Tavtigian S., Bennett L.M., Haugen-Strano A., Swensen J., Miki Y., Eddington K., McClure M., Frye C., Weaver-Feldhaus J., Ding W., Gholami Z., Soderkvist P., Terry L., Jhanwar S., Berchuck A., Iglehart J.D., Marks J., Ballinger D.G., Barrett J.C., Skolnick M.H., Kamb A., Wiseman R. BRCA1 mutations in primary breast and ovarian carcinomas. Science (Washington, DC, US) 1994, 266:120-122.
11. van Mansfeld A.D., Bos J.L. PCR-based approaches for detection of mutated ras genes. Genome Res. 1992, 1:211-216.