Efficacy of an in-house polymerase chain reaction assay for rapid diagnosis of Mycobacterium tuberculosis in patients with tubercular lymphadenitis: Comparison with fine needle aspiration cytology and conventional techniques

Indian Journal of Pathology and Microbiology

Volumn 53, Issue 4, 2010, Pages 714-717

  Sharma, Meera ^a   Sethi, Sunil ^a   Mishra, Ashwini Kumar ^a   Chatterjee, Shiv Sekhar ^a   Wanchu, Ajay ^a   Nijhawan, Raje ^a  

^a POSTGRADUATE INSTITUTE OF MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

Fine needle aspiration cytology; IS6110 PCR; tubercular lymphadenitis

Indexed keywords

BACTERIAL DNA;

ADOLESCENT; ADULT; AGED; ARTICLE; ASPIRATION BIOPSY; BACTERIUM CULTURE; BACTERIUM ISOLATION; CHILD; CLINICAL EFFECTIVENESS; CYTOPATHOLOGY; FEMALE; HISTOPATHOLOGY; HUMAN; INTERMETHOD COMPARISON; LYMPHADENOPATHY; MAJOR CLINICAL STUDY; MALE; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; TREATMENT RESPONSE; TUBERCULOUS LYMPHADENITIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIOLOGICAL TECHNIQUES; BIOPSY, FINE-NEEDLE; CHILD; CHILD, PRESCHOOL; FEMALE; HISTOCYTOCHEMISTRY; HUMANS; INFANT; LYMPH NODES; MALE; MICROSCOPY; MIDDLE AGED; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; TUBERCULOSIS, LYMPH NODE; YOUNG ADULT;

EID: 78149391211     PISSN: 03774929     EISSN: 09745130     Source Type: Journal    
DOI: 10.4103/0377-4929.72049     Document Type: Article

References (11)

1. Powell DA. Tuberculous Lymphadenitis. Schlossberg Tuberculosis andNontuberculous Mycobacterial Infections. 4th ed. Philadelphia, USA: W.B Saunders Company; 1999. p. 186-94.
2. Iseman MD. Extrapulmonary tuberculosis in adults. A clinician's guide to tuberculosis. Philadelphia, USA: Lippincott Williams and Wilkins; 2000. p. 145-98.
3. Bayazit YA, Bayazit N, Namidurub M. Mycobacterial Cervical Lymphadenitis. ORL J Otorhinolaryngol Relat Spec 2004;66:275-80.
4. Khan RA, Wahab S, Chana RS, Naseem S, Siddique S. Children with significant cervical lymphadenopathy: Clinicopathological analysis and role of fine-needle aspiration in Indian setup. J Pediatr (Rio J) 2008;84:449-54.
5. Singh KK, Muralidhar M, Kumar A, Chattopadhyaya TK, Kapila K, Singh MK, et al. Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy. J Clin Pathol 2000;53:355-61.
6. Pahwa R, Hedau S, Jain S, Jain N, Arora VM, Kumar N, et al. Assessment of possible tuberculous lymphadenopathy by PCR compared to nonmolecular methods. J Med Microbiol 2005;54:873-8.
7. Sekar B, Selvaraj L, Alexis A, Ravi S, Arunagiri K, Rathinavel L. The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis. Indian J Med Microbiol 2008;26:352-5.
8. Guidelines on HIV testing. March 2007. National AIDS control authority. Ministry of Health and Family Welfare. Government of India.
9. Van Soolingen D, Hermans PW, Hass De PE, Soll DR, Embden JD. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex: Evaluation of insertion sequence dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol 1991;29:2578-86.
10. Rodrigues C, Nukula R, Menon S, Hakimiyan A, Mehta AP. DNA amplification of IS6110 in rapid detection of Mycobacterium tuberculosis. Indian J Med Microbiol 1997;4:167-71.
11. Negi SS, Anand R, Pasha ST, Gupta S, Blasir SF, Khare S, et al. Diagnostic potential of IS6110, 38 kDa, 65 kDa and 85B sequence based polymerase chain reaction in the diagnosis of Mycobacterium tuberculosis in clinical samples. Indian J Med Microbiol 2007;25:43-9.