Detection of elm yellows phytoplasma in elms and insects using real-time PCR

Plant Disease

Volumn 94, Issue 11, 2010, Pages 1355-1360

  Herath, Padmini ^a   Hoover, Gregory A ^a   Angelini, Elisa ^b   Moorman, Gary W ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA EXTRACTION; ILLINOIS; PENNSYLVANIA STATE UNIVERSITY; PHYLOGENETIC GROUPS; PHYTOPLASMAS; REAL-TIME PCR; REAL-TIME PCR ASSAY; REAL-TIME PCR METHOD; REAL-TIME POLYMERASE CHAIN REACTION;

CHLOROPHYLL; DNA; DNA SEQUENCES; GENES; PROBES;

POLYMERASE CHAIN REACTION;

ASTER YELLOWS PHYTOPLASMA; CANDIDATUS PHYTOPLASMA; CANDIDATUS PHYTOPLASMA ULMI; HEXAPODA; ULMUS; ULMUS AMERICANA;

EID: 78149304701     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-12-09-0783     Document Type: Article

References (31)

1. Angelini, E., Bianchi, G. L., Filippin, L., Morassutti, C., and Borgo, M. 2007. A new TaqMan method for the identification of phytoplasmas associated with grapevine yellows by real-time PCR assay. J. Microbiol. Methods 68:613-622.
2. Bai, X., Correa, V. R., Toruno, T. Y., Ammar, E. D., Kamoun, S., and Hogenhout, S. A. 2009. AY-WB Phytoplasma secretes a protein that targets plant cell nuclei. Mol. Plant- Microbe Interact. 22:18-30.
3. Baker, W. L. 1948. Transmission by leafhoppers of the virus causing phloem necrosis of American elm. Science 108:307-308.
4. Baric, S., and Dalla-Via, J. 2004. A new approach to apple proliferation detection: A highly sensitive real-time PCR assay. J. Microbiol. Methods 57:135-145.
5. Bertelli, E., Tegli, S., Sfalanga, A., and Surico, G. 2002. Detection of phytoplasmal DNA in flowers and seeds from elm trees infected with elm yellows. Phytopathol. Mediterr. 41:259-265.
6. Boudon-Padieu, E., Larrue, J., Clair, D., Hourdel, J., Jeanneau, A., Sforza, R., and Collin, E. 2004. Detection and prophylaxis of elm yellows phytoplasma in France. Invest. Agrar. Sist. Recur. For. 13:71-80.
7. Bustin, S. 2004. AZ of Quantitative PCR. International University Line, La Jolla, CA.
8. Carraro, L., Ferrini, F., Ermacora, P., Loi, N., Martini, M., and Osler, R. 2004. Macropsis mendax as a vector of elm yellows phytoplasma of Ulmus species. Plant Pathol. 53:90-95.
9. Christensen, N. M., Nicolaisen, M., Hansen, M., and Schulz, A. 2004. Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging. Mol. Plant- Microbe Interact. 17:1175-1184.
10. Chuche, J., and Thiéry, D. 2009. Cold winter temperatures condition the egg-hatching dynamics of a grape disease vector. Naturwissenschaften 96:827-834
11. Delong, D. 1948. The leafhoppers, or Cicadellidae, of Illinois (Eurymelinae-Balcluthinae). Ill. Nat. Hist. Surv. Bull. 24:91-376.
12. Delong, D. M. 1939. A review of the genus Scaphoideus. Proc. Entomol. Soc. Wash. 41:33-45.
13. Dietrich, C. 2005. Keys to the families of Cicadomorpha and subfamilies and tribes of Cicadellidae (Hemiptera: Auchenorrhyncha). Fla. Entomol. 88:502-517.
14. Drooz, A. 1985. Insects of eastern forests. U. S. Dep. Agric. For. Serv. Misc. Publ. 1426:608.
15. Galetto, L., Bosco, D., and Marzachi, C. 2005. Universal and group-specific real-time PCR diagnosis of flavescence doree (16Sr-V), bois noir (16Sr-XII) and apple proliferation (16Sr- X) phytoplasmas from field-collected plant hosts and insect vectors. Ann. Appl. Biol. 147:191-201.
16. Ginzinger, D. 2002. Gene quantification using real-time quantitative PCR an emerging technology hits the mainstream. Exp. Hematol. 30:503-512.
17. Green, M., Thompson, D., and MacKenzie, D. 1999. Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. Plant Dis. 83:482-485.
18. Hamilton, K. G. A. 1985. Leafhoppers of ornamental and fruit trees in Canada. Agric. Can. Publ. 1779/E:1-71.
19. Hogenhout, S. A., Oshima, K., Ammar, E. L. D., Kakizawa, S., Kingdom, H. N., and Namba, S. 2008. Phytoplasmas: Bacteria that manipulate plants and insects. Mol. Plant Pathol. 9:403-423.
20. Hren, M., Boben, J., Rotter, A., Kralj, P., Gruden, K., and Ravnikar, M. 2007. Real-time PCR detection systems for Flavescence doree and Bois noir phytoplasmas in grapevine: Comparison with conventional PCR detection and application in diagnostics. Plant Pathol. 56:785-796.
21. Jacobs, K., Lee, I., Griffiths, H., Miller, F., Jr., and Bottner, K. 2003. A new member of the clover proliferation phytoplasma group (16SrVI) associated with elm yellows in Illinois. Plant Dis. 87:241-246.
22. Lee, I. M., Davis, R. E., and Gundersen- Rindal, D. E. 2000. Phytoplasma: Phytopathogenic mollicutes. Annu. Rev. Microbiol. 54:221-255.
23. Lee, I. M., Martini, M., Marcone, C., and Zhu, S. F. 2004. Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of Candidatus Phytoplasma ulmi for the phytoplasma associated with elm yellows. International J. Syst. Evol. Microbiol. 54:337-347.
24. Matteoni, J., and Sinclair, W. 1988. Elm yellows and ash yellows. Pages 19-31 in: Tree Mycoplasma Diseases and Epidemiology. C. Hiruki, ed. University of Alberta Press, Edmonton, AB, Canada.
25. Osman, F., and Rowhani, A. 2006. Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan). J. Virol. Methods 133:130-136.
26. Sinclair, W., and Lyon, H. 2005. Diseases of Trees and Shrubs. Comstock Publishing Associates, New York.
27. Sinclair, W. A., Townsend, A. M., Griffiths, H. M., and Whitlow, T. H. 2000. Responses of six Eurasian Ulmus cultivars to a North American elm yellows phytoplasma. Plant Dis. 84:1266-1270.
28. Swingle, R. 1938. A phloem necrosis of elm. Phytopathology 28:757-759.
29. Torres, E., Bertolini, E., Cambra, M., Montón, C., and Martín, M. P. 2005. Real-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16SrX) group. Mol. Cell. Probes 19:334-340.
30. Townsend, A. M., Bentz, S. E., and Douglass, L. W. 2005. Evaluation of 19 American elm clones for tolerance to Dutch elm disease. J. Environ. Hortic. 23:21-24.
31. Wei, W., Kakizawa, S., Suzuki, S., Jung, H., Nishigawa, H., Miyata, S., Oshima, K., Ugaki, M., Hibi, T., and Namba, S. 2004. In planta dynamic analysis of onion yellows phytoplasma using localized inoculation by insect transmission. Phytopathology 94:244-250.