Altered gene expression in human adipose stem cells cultured with fetal bovine serum compared to human supplements

Tissue Engineering - Part A

Volumn 16, Issue 11, 2010, Pages 3467-3484

  Bieback, Karen ^a   Ha, Viet Anh Thu ^a   Hecker, Andrea ^a   Grassl, Melanie ^a,b   Kinzebach, Sven ^a   Solz, Hermann ^c   Sticht, Carsten ^a   Klüter, Harald ^a   Bugert, Peter ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MEDICAL FACULTY MANNHEIM HEIDELBERG UNIVERSITY   (Germany)

^c Mannheimer Clinic for Plastic Surgery   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; BODY FLUIDS; CELL CULTURE; FLOWCHARTING; POLYMERASE CHAIN REACTION; STEM CELLS; TISSUE;

ACTIVATED PLATELETS; ADIPOGENIC; ADIPOSE TISSUE; BLOOD GROUP; COMMONLY USED; EX-VIVO; EXTRACELLULAR MATRICES; FETAL BOVINE SERUM; GENE EXPRESSION ANALYSIS; GROWTH PATTERNS; HUMAN ADIPOSE; HUMAN SERUM; IN-CELL; MESENCHYMAL STROMAL CELLS; MICROARRAY-BASED SCREENING; OSTEOGENIC DIFFERENTIATION; PROTEIN ANALYSIS; PROTEIN EXPRESSIONS; REGULATORY AGENCIES; THERAPEUTIC APPLICATION; THERAPEUTIC POTENTIALS;

GENE EXPRESSION;

BOVINAE;

RNA; TRYPSIN;

ADIPOGENESIS; ADIPOSE TISSUE; ADULT; ANIMAL; ARTICLE; BONE DEVELOPMENT; CATTLE; CELL CULTURE; CELL PROLIFERATION; CELL SHAPE; COMPARATIVE STUDY; CULTURE MEDIUM; CYTOLOGY; DNA MICROARRAY; DOWN REGULATION; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; HUMAN; KINETICS; MESENCHYMAL STEM CELL; METABOLISM; MIDDLE AGED; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERUM; UPREGULATION;

ADIPOGENESIS; ADIPOSE TISSUE; ADULT; ANIMALS; CATTLE; CELL PROLIFERATION; CELL SHAPE; CELLS, CULTURED; CULTURE MEDIA; DOWN-REGULATION; GENE EXPRESSION REGULATION; HUMANS; KINETICS; MESENCHYMAL STEM CELLS; MIDDLE AGED; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OSTEOGENESIS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SERUM; TRYPSIN; UP-REGULATION;

EID: 78149252001     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0727     Document Type: Article

References (39)

1. Bianco, P., Robey, P.G., and Simmons, P.J. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2, 313, 2008.
2. Bieback, K., Schallmoser, K., Klueter, H., and Strunk, D. Clinical protocols for the isolation and expansion of mes-enchymal stromal cells. Transfus Med Hemother 35, 286, 2008.
3. Mannello, F., and Tonti, G.A. Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells 25, 1603, 2007.
4. Honn, K.V., Singley, J.A., and Chavin, W. Fetal bovine serum: a multivariate standard. Proc Soc Exp Biol Med 149, 344, 1975.
5. Heiskanen, A., Satomaa, T., Tiitinen, S., Laitinen, A., Man-nelin, S., Impola, U., Mikkola, M., Olsson, C., Miller-Podraza, H., Blomqvist, M., Olonen, A., Salo, H., Lehenkari, P., Tuuri, T., Otonkoski, T., Natunen, J., Saarinen, J., and Laine, J. N-glycolylneuraminic acid xenoantigen contamination of human embryonic and mesenchymal stem cells is substantially reversible. Stem Cells 25, 197, 2007.
6. Sundin, M., Ringden, O., Sundberg, B., Nava, S., Gother-strom, C., and Le Blanc, K. No alloantibodies against mes-enchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients. Haematologica 92, 1208, 2007.
7. Note for Guidance on the Use of Bovine Serum in the Manufacture of Human Medicinal Products. In: EMEA, ed. EMEA CPMP/BWP/1793/02, 2003. www.ema.europa.eu/ docs/en-GB/document-library/Scientific-guideline/2009/ 09/WC500003675.pdf
8. Stute, N., Holtz, K., Bubenheim, M., Lange, C., Blake, F., and Zander, A.R. Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp He-matol 32, 1212, 2004.
9. Phadnis, S.M., Joglekar, M.V., Venkateshan, V., Ghaskadbi, S.M., Hardikar, A.A., and Bhonde, R.R. Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells. In Vitro Cell Dev Biol Anim 42, 283, 2006.
10. Schallmoser, K., Bartmann, C., Rohde, E., Reinisch, A., Ka-shofer, K., Stadelmeyer, E., Drexler, C., Lanzer, G., Linkesch, W., and Strunk, D. Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion 47, 1436, 2007.
11. Muller, A.M., Davenport, M., Verrier, S., Droeser, R., Alini, M., Bocelli-Tyndall, C., Schaefer, D.J., Martin, I., and Scher-berich, A. Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue. Tissue Eng Part A 15, 869, 2009.
12. Sun, X., Gan, Y., Tang, T., Zhang, X., and Dai, K. In vitro proliferation and differentiation of human mesenchymal stem cells cultured in autologous plasma derived from bone marrow. Tissue Eng Part A 14, 391, 2008.
13. Prins, H.J., Rozemuller, H., Vonk-Griffioen, S., Verweij, V.G., Dhert, W.J., Slaper-Cortenbach, I.C., and Martens, A.C. Bone-forming capacity of mesenchymal stromal cells when cultured in the presence of human platelet lysate as substitute for fetal bovine serum. Tissue Eng Part A 15, 3741, 2009.
14. McKenna, D.H., Jr., Sumstad, D., Bostrom, N., Kadidlo, D.M., Fautsch, S., McNearney, S., Dewaard, R., McGlave, P.B., Weisdorf, D.J., Wagner, J.E., McCullough, J., and Miller, J.S. Good manufacturing practices production of natural killer cells for immunotherapy: a six-year single-institution experience. Transfusion 47, 520, 2007.
15. Kocaoemer, A., Kern, S., Klueter, H., and Bieback, K. Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 25, 1270, 2007.
16. Bieback, K., Hecker, A., Kocaomer, A., Lannert, H., Schallmoser, K., Strunk, D., and Klueter, H. Human alternatives to fetal bovine serum for the expansion of mesen-chymal stromal cells from bone marrow. Stem Cells 27, 2331, 2009.
17. Spaeth, E., Klopp, A., Dembinski, J., Andreeff, M., and Marini, F. Inflammation and tumor microenvironments: defining the migratory itinerary of mesenchymal stem cells. Gene Ther 15, 730, 2008.
18. Baudin, B., Bruneel, A., Bosselut, N., and Vaubourdolle, M. A protocol for isolation and culture of human umbilical vein endothelial cells. Nat Protoc 2, 481, 2007.
19. Greenbaum, D., Colangelo, C., Williams, K., and Gerstein, M. Comparing protein abundance and mRNA expression levels on a genomic scale. Genome Biol 4, 117, 2003.
20. Hogan, J.C., and Stephens, J.M. Effects of leukemia inhibitory factor on 3T3-L1 adipocytes. J Endocrinol 185, 485, 2005.
21. Karahuseyinoglu, S., Kocaefe, C., Balci, D., Erdemli, E., and Can, A. Functional structure of adipocytes differentiated from human umbilical cord stroma-derived stem cells. Stem Cells 26, 682, 2008.
22. http://medgen.ugent.be/*jvdesomp/genorm/.
23. Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., and Speleman, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3, RESEARCH0034, 2002.
24. Lange, C., Cakiroglu, F., Spiess, A.N., Cappallo-Obermann, H., Dierlamm, J., and Zander, A.R. Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. J Cell Physiol 213, 18, 2007.
25. Guideline on Human Cell-Based Medicinal Products. EMEA/CHMP/410869/2006, 2008. www.ema.europa.eu/ docs/en-GB/document-library/Scientific-guideline/2009/ 09/WC500003894.pdf
26. Rada, T., Reis, R.L., and Gomes, M.E. Novel method for the isolation of adipose stem cells (ASCs). J Tissue Eng Regen Med 3, 158, 2009.
27. Lindroos, B., Aho, K.L., Kuokkanen, H., Raety, S., Huhtala, H., Lemponen, R., Yli-Harja, O., Suuronen, R., and Mietti-nen, S. Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum. Tissue Eng Part A 16, 2281, 2010.
28. Shahdadfar, A., Fronsdal, K., Haug, T., Reinholt, F.P., and Brinchmann, J.E. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 23, 1357, 2005.
29. Dahl, J.A., Duggal, S., Coulston, N., Millar, D., Melki, J., Shahdadfar, A., Brinchmann, J.E., and Collas, P. Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum. Int J Dev Biol 52, 1033, 2008.
30. Barry, F., Boynton, R.E., Liu, B., and Murphy, J.M. Chon-drogenic differentiation of mesenchymal stem cells from bone marrow: differentiation- dependent gene expression of matrix components. Exp Cell Res 268, 189, 2001.
31. Goessler, U.R., Bugert, P., Bieback, K., Bag, S., Sadick, H., Klueter, H., Hormann, K., and Riedel, F. [A comparison of the gene expression patterns of human chondrocytes and chondrogen differentiated mesenchymal stem cells for tissue engineering]. HNO 54, 258, 2006.
32. Tormin, A., Brune, J.C., Olsson, E., Valcich, J., Neuman, U., Olofsson, T., Jacobsen, S.E., and Scheding, S. Characterization of bone marrow-derived mesenchymal stromal cells (MSC) based on gene expression profiling of functionally defined MSC subsets. Cytotherapy 11, 114, 2009.
33. Parker, A.M., Shang, H., Khurgel, M., and Katz, A.J. Low serum and serum-free culture of multipotential human adipose stem cells. Cytotherapy 9, 637, 2007.
34. Chevallier, N., Anagnostou, F., Zilber, S., Bodivit, G., Maurin, S., Barrault, A., Bierling, P., Hernigou, P., Layrolle, P., and Rouard, H. Osteoblastic differentiation of human mesenchymal stem cells with platelet lysate. Biomaterials 31, 270, 2009.
35. Goessler, U.R., Bieback, K., Bugert, P., Heller, T., Sadick, H., Hormann, K., and Riedel, F. In vitro analysis of integrin expression during chondrogenic differentiation of mesen-chymal stem cells and chondrocytes upon dedifferentiation in cell culture. Int J Mol Med 17, 301, 2006.
36. Lee, R.H., Seo, M.J., Pulin, A.A., Gregory, C.A., Ylostalo, J., and Prockop, D.J. The CD34-like protein PODXL and alpha6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice. Blood 113, 816, 2009.
37. Lisowska-Myjak, B., Pachecka, J., Kaczynska, B., Miszkurka, G., and Kadziela, K. Serum protease inhibitor concentrations and total antitrypsin activity in diabetic and non-diabetic children during adolescence. Acta Diabetol 43, 88, 2006.
38. Schechter, N.M., and Plotnick, M.I. Measurement of the kinetic parameters mediating protease-serpin inhibition. Methods 32, 159, 2004.
39. Chow, T.W., McIntire, L.V., and Peterson, D.M. Importance of plasma fibronectin in determining PFP and PRP clot mechanical properties. Thromb Res 29, 243, 1983.