Regulation of 3-phosphoinositide-dependent protein kinase 1 activity by homodimerization in live cells

Science Signaling

Volumn 3, Issue 145, 2010, Pages

  Masters, Thomas A ^a,b   Calleja, Véronique ^a   Armoogum, Daven A ^b   Marsh, Richard J ^b   Applebee, Christopher J ^a   Laguerre, Michel ^c   Bain, Angus J ^b   Larijani, Banafshé ^a  

^a THE FRANCIS CRICK INSTITUTE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HOMODIMER; PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE 1; PLECKSTRIN; PROTEIN KINASE B; 3 PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE; PROTEIN KINASE INHIBITOR; PROTEIN SERINE THREONINE KINASE;

ARTICLE; DIMERIZATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME REGULATION; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN DOMAIN; ANIMAL; CELL STRAIN 3T3; CELL STRAIN COS1; CERCOPITHECUS; DRUG ANTAGONISM; ENZYME ACTIVATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETICS; HUMAN; METABOLISM; MOUSE; MUTATION; PHYSIOLOGY; PROTEIN MULTIMERIZATION; PROTEIN TERTIARY STRUCTURE;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; ENZYME ACTIVATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; MICE; MUTATION; NIH 3T3 CELLS; PROTEIN KINASE INHIBITORS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES;

EID: 78049519112     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.2000738     Document Type: Article

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44. Acknowledgments: We are grateful to P. J. Parker for critical reading of the manuscript and for scientific discussions. We are indebted to S. Kisakye-Nambozo, A. Borg, R. George, and S. Kjaer for help with protein expression and purification. Funding: This research project was funded by Cancer Research UK core funding and Engineering and Physical Science Council (EPSRC) grant EP/C536134/1. T.A.M. gratefully acknowledges the EPSRC doctoral training centre PhD programme at the University College London Centre for Mathematics and Physics in the Life Sciences and Experimental Biology (CoMPLEX) for financial support. Author contributions: A.J.B. and B.L. directed the research; T.A.M., V.C., and B.L. designed and performed the FLIM experiments; T.A.M. and V.C. designed the molecular biology tools; T.A.M. and V.C. performed the biochemical experiments; C.J.A. and B.L. developed the two-photon FLIM apparatus; A.J.B. designed the in vitro experiments; T.A.M., D.A.A., and R.J.M. planned and performed the in vitro experiments; T.A.M., R.J.M., and A.J.B. analyzed the in vitro data; M.L. performed the molecular modeling; T.A.M., V.C., D.A.A., R.J.M., M.L., A.J.B., and B.L. were involved in scientific discussions; and T.A.M., V.C., A.J.B., and B.L. wrote the manuscript. Competing interests: The authors declare that they have no competing interests.