Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells

Methods in Molecular Biology

Volumn 657, Issue , 2010, Pages 249-257

  Hagiwara, Haruo ^a   Aoki, Takeo ^a   Suzuki, Takeshi ^a   Takata, Kuniaki ^a  

^a GUNMA UNIVERSITY   (Japan)

Author keywords

Colloidal gold; Cryosectioning technique; Cultured cells; Double immunolabeling; Tokuyasu method

Indexed keywords

ANIMALIA;

ANTIBODY; ANTIGEN; CAV1 PROTEIN, HUMAN; CAVEOLIN 1; COLLOIDAL GOLD; GELATIN; RAB PROTEIN;

ARTICLE; CELL CULTURE; CHEMISTRY; CYTOLOGY; ENDOTHELIUM CELL; HISTOLOGY; HUMAN; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METHODOLOGY; PARTICLE SIZE; STAINING; ULTRAMICROTOMY; ULTRASTRUCTURE;

ANTIBODIES; ANTIGENS; CAVEOLIN 1; CELLS, CULTURED; CRYOULTRAMICROTOMY; ENDOTHELIAL CELLS; GELATIN; GOLD COLLOID; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; PARTICLE SIZE; RAB5 GTP-BINDING PROTEINS; STAINING AND LABELING; TISSUE EMBEDDING;

EID: 78049519009     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-783-9_20     Document Type: Article

References (7)

1. Tokuyasu, K. T. (1978) A study of positive staining of ultrathin frozen sections. J. Ultrastruct. Res. 63, 287-307. (Pubitemid 8405156)
2. Tokuyasu, K. T. (1986) Application of cryoultramicrotomy to immunocytochemistry. J. Microsc. 143, 139-149.
3. Tokuyasu, K. T. (1989) Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy. Histochem. J. 21, 163-171. (Pubitemid 19138135)
4. Liou, W., Geuze, H. J., and Slot, J. W. (1996) Improving structural integrity of cryosections for immunogold labeling. Histochem. Cell Biol. 106, 41-58. (Pubitemid 26291492)
5. Webster, P. and Webster, A. (2007) Cryosectioning fixed and cryoprotected biological material for immunocytochemistry. Methods Mol. Biol. 369, 257-289. (Pubitemid 350183451)
6. Fujimoto, T., Tokuyasu, K. T., and Singer, S. J. (1987) Direct morphological demonstration of the coexistence of vimentin and desmin in the same intermediate filaments of vascular smooth muscle cells. J. Submicrosc. Cytol. 19, 1-9. (Pubitemid 17081277)
7. Aoki, T., Hagiwara, H., Matsuzaki, T., Suzuki, T., and Takata, K. (2007) Internalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells. Anat. Sci. Int. 82, 82-97. (Pubitemid 46845350)