Evaluation of bioaerosol sampling techniques for Legionella pneumophila coupled with culture assay and quantitative PCR

Journal of Aerosol Science

Volumn 41, Issue 12, 2010, Pages 1055-1065

  Chang, Ching Wen ^a   Chou, Fang Chen ^a   Hung, Pei Yu ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Bioaerosol; Culture; Legionella pneumophila; Quantitative PCR; Sampling

Indexed keywords

CELL CULTURE; DEIONIZED WATER; ESCHERICHIA COLI; MIXTURES; POLYMERASE CHAIN REACTION; RECOVERY; SAMPLING;

BACTERIAL SPECIES; BIOAEROSOLS; COLLECTION EFFICIENCY; LEGIONELLA PNEUMOPHILA; ORDERS OF MAGNITUDE; QUANTITATIVE PCR; SAMPLING METHOD; SAMPLING TECHNIQUE;

AEROSOLS;

AEROSOL; BACTERIUM; CAUSE OF DEATH; PATHOGEN; PNEUMONIA; POLYMERASE CHAIN REACTION; SAMPLING;

AEROSOL; AGAR MEDIUM; AIR SAMPLER; AIRBORNE BACTERIUM; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; ESCHERICHIA COLI; LEGIONELLA PNEUMOPHILA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LEGIONELLA PNEUMOPHILA;

EID: 78049500587     PISSN: 00218502     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jaerosci.2010.09.002     Document Type: Article

References (37)

1. Agranovski V., Ristovski Z., Hargreaves M., Blackall P.J., Morawska L. Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation. Journal of Aerosol Science 2003, 34:301-317.
2. An H.R., Mainelis G., White L. Development and calibration of real-time PCR for quantification of airborne microorganisms in air samples. Atmospheric Environment 2006, 40:7924-7939.
3. Bauer M., Mathieu L., Deloge-Abarkan M., Remen T., Tossa P., Hartemann P., et al. Legionella bacteria in shower aerosols increase the risk of Pontiac fever among older people in retirement homes. Journal of Epidemiology and Community Health 2008, 62:913-920.
4. Blatny J.M., Reif B.A., Skogan G., Andreassen O., Hoiby E.A., Ask E., et al. Tracking airborne Legionella and Legionella pneumophila at a biological treatment plant. Environmental Science and Technology 2008, 42:7360-7367.
5. Breiman R.F., Cozen W., Fields B.S., Mastro T.D., Carr S.J., Spika J.S., et al. Role of air sampling in investigation of an outbreak of Legionnaires' disease associated with exposure to aerosols from an evaporative condenser. The Journal of Infectious Diseases 1990, 161:1257-1261.
6. Buchbinder S., Trebesius K., Heesemann J. Evaluation of detection of Legionella spp. in water samples by fluorescence in situ hybridization, PCR amplification and bacterial culture. International Journal of Medical Microbiology 2002, 292:241-245.
7. Chang C.W., Hwang Y.H., Cheng W.Y., Chang C.P. Effects of chlorination and heat disinfection on long-term starved Legionella pneumophila in warm water. Journal of Applied Microbiology 2007, 102:1636-1644.
8. Chen N.T., Chang C.W. Rapid quantification of viable Legionella in water and biofilm using ethidium monoazide coupled with real-time quantitative PCR. Journal of applied microbiology 2010, 109:623-634.
9. Chen P.S., Li C.S. Sampling performance for bioaerosols by flow cytometry with fluorochrome. Aerosol Science and Technology 2005, 39:231-237.
10. Deloge-Abarkan M., Ha T.L., Robine E., Zmirou-Navier D., Mathieu L. Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH). Journal of Environmental Monitoring 2007, 9:91-97.
11. Ding P.H., Wang C.S. Effect of sampling time on the sampling efficiency of all-glass impinger-30 samplers for E. coli aerosol. Journal of Aerosol Science 1997, 28:671-672.
12. Dutil S., Veillette M., Meriaux A., Lazure L., Barbeau J., Duchaine C. Aerosolization of mycobacteria and legionellae during dental treatment: low exposure despite dental unit contamination. Environmental Microbiology 2007, 9:2836-2843.
13. Gilmour M.W., Bernard K., Tracz D.M., Olson A.B., Corbett C.R., Burdz T., et al. Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada. Journal of Medical Microbiology 2007, 56:336-341.
14. Ishimatsu S., Miyamoto H., Hori H., Tanaka I., Yoshida S. Sampling and detection of Legionella pneumophila aerosols generated from an industrial cooling tower. The Annals of Occupational Hygiene 2001, 45:421-427.
15. Jerome V., Hermann M., Hilbrig F., Freitag R. Development of a fed-batch process for the production of a dye-linked formaldehyde dehydrogenase in Hyphomicrobium zavarzinii ZV 580. Applied Microbiology and Biotechnology 2007, 77:779-788.
16. Kane A.V., Plaut A.G. Unique susceptibility of Helicobacter pylori to simethicone emulsifiers in alimentary therapeutic agents. Antimicrobial Agents and Chemotherapy 1996, 40:500-502.
17. Klappenbach J.A., Saxman P.R., Cole J.R., Schmidt T.M. rrndb: the ribosomal RNA operon copy number database. Nucleic Acids Research 2001, 29:181-184.
18. Kuchta J.M., States S.J., McNamara A.M., Wadowsky R.M., Yee R.B. Susceptibility of Legionella pneumophila to chlorine in tap water. Applied and Environmental Microbiology 1983, 46:1134-1139.
19. Li C.S., Lin Y.C. Sampling performance of impactors for bacterial bioaerosols. Aerosol Science and Technology 1999, 30:280-287.
20. Li C.S., Tseng C.C., Lai H.H., Chang C.W. Ultraviolet germicidal irradiation and titanium dioxide photocatalyst for controlling Legionella pneumophila. Aerosol Science and Technology 2003, 37:961-966.
21. Lin X., Reponen T.A., Willeke K., Wang Z., Grinshpun S.A., Trunov M. Survival of airborne microorganisms during swirling aerosol collection. Aerosol Science and Technology 2000, 32:184-196.
22. Macher J.M. Positive-hole correction of multiple-jet impactors for collecting viable microorganisms. American Industrial Hygiene Association Journal 1989, 50:561-568.
23. Macher, J. M., & Burge, H. A. (2001). Sampling biological aerosols. In B. S. Cohen, & C. S. McCammon (Eds.), Air sampling instruments for evaluation of atmospheric contaminants (9th ed.). American conference of government industrial hygienists, Cincinnati, OH (pp. 661-701).
24. Mainelis G., Tabayoyong M. The effect of sampling time on the overall performance of portable microbial impactors. Aerosol Science and Technology 2010, 44:75-82.
25. Mathieu L., Robine E., Deloge-Abarkan M., Ritoux S., Pauly D., Hartemann P., et al. Legionella bacteria in aerosols: sampling and analytical approaches used during the legionnaires disease outbreak in Pas-de-Calais. The Journal of Infectious Diseases 2006, 193:1333-1335.
26. Medema G., Wullings B., Roeleveld P., van der Kooij D. Risk assessment of Legionella and enteric pathogens in sewage treatment works. Water Science and Technology 2004, 4:125-132.
27. Miller L.A., Beebe J.L., Butler J.C., Martin W., Benson R., Hoffman R.E., et al. Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever. The Journal of Infectious Diseases 1993, 168:769-772.
28. Morio F., Corvec S., Caroff N., Le Gallou F., Drugeon H., Reynaud A. Real-time PCR assay for the detection and quantification of Legionella pneumophila in environmental water samples: Utility for daily practice. International Journal of Hygiene and Environmental Health 2008, 211:403-411.
29. Nguyen T.M., Ilef D., Jarraud S., Rouil L., Campese C., Che D., et al. A community-wide outbreak of legionnaires disease linked to industrial cooling towers-How far can contaminated aerosols spread?. The Journal of Infectious Diseases 2006, 193:102-111.
30. Pascual L., Perez-Luz S., Amo A., Moreno C., Apraiz D., Catalan V. Detection of Legionella pneumophila in bioaerosols by polymerase chain reaction. Canadian Journal of Microbiology 2001, 47:341-347.
31. Polaczyk A.L., Narayanan J., Cromeans T.L., Hahn D., Roberts J.M., Amburgey J.E., et al. Ultrafiltration-based techniques for rapid and simultaneous concentration of multiple microbe classes from 100-L tap water samples. Journal of Microbiological Methods 2008, 73:92-99.
32. Rule A.M., Kesavan J., Schwab K.J., Buckley T.J. Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans. Environmental Science and Technology 2007, 41:2467-2472.
33. Rule A.M., Kesavan J., Schwab K.J., Buckley T.J. Assessment of bioaerosol generation and sampling efficiency based on Pantoea agglomerans. Aerosol Science and Technology 2009, 43:620-628.
34. Thorne P.S., Kiekhaefer M.S., Whitten P., Donham K.J. Comparison of bioaerosol sampling methods in barns housing swine. Applied and Environmental Microbiology 1992, 58:2543-2551.
35. Willeke K., Lin X., Grinshpun S.A. Improved aerosol collection by combined impaction and centrifugal motion. Aerosol Science and Technology 1998, 28:439-456.
36. Yao M., Mainelis G. Investigation of cut-off sizes and collection efficiencies of portable microbial samplers. Aerosol Science and Technology 2006, 40:595-606.
37. Yao M., Mainelis G. Analysis of portable impactor performance for enumeration of viable bioaerosols. Journal of Occupational and Environmental Hygiene 2007, 4:514-524.