Microfluidics-based immunoassays by using an integrated fluorescence detection system

Microsystem Technologies

Volumn 16, Issue 12, 2010, Pages 2049-2055

  Chen, Xing ^a   Cui, Dafu ^a   Li, Hui ^a   Cai, Haoyuan ^a   Sun, Jianhan ^a   Chen, Jian ^a  

^a INSTITUTE OF ELECTRONICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY CONCENTRATION; ANTIBODY IMMOBILIZATION; CLINICAL TESTING; CORRELATION COEFFICIENT; CRITICAL PARAMETER; DETECTION RANGE; DETECTION SYSTEM; EXCITATION LIGHT; FLUORESCENCE IMMUNOASSAY; FLUORESCENT LIGHT; HUMAN IGG; INTEGRATED FLUORESCENCE DETECTION; MICRO PUMP; MICROFLUIDIC CHIP; OPTIMAL CONDITIONS; POINT OF CARE; SANDWICH IMMUNOASSAY; SURFACE MODIFICATION METHODS;

ANTIBODIES; DYES; FLUIDIC DEVICES; FLUORESCENCE; MICROFLUIDICS; OPTICAL FIBERS; OPTIMIZATION; PUMPS;

CHEMICAL DETECTION;

EID: 78049468411     PISSN: 09467076     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00542-010-1150-5     Document Type: Article

References (19)

1. E Brown T McDermott K Bloch A McCollom 1996 Defining the smallest analyte concentration an immunoassay can measure Clin Chem 42 6 893 903
2. S Cesaro-Tadic G Dernick D Juncker G Buurman H Kropshofer B Michel C Fattinger E Delamarche 2004 High-sensitivity miniaturized immunoassays for tumor necrosis factor α using microfluidic systems Lab Chip 4 6 563 569 10.1039/b408964b
3. M Chabinyc D Chiu J McDonald A Stroock J Christian A Karger G Whitesides 2001 An integrated fluorescence detection system in poly (dimethylsiloxane) for microfluidic applications Anal Chem. 73 4491 4498 10.1021/ac010423z
4. X Chen D Cui C Liu H Li J Chen 2007 Continuous flow microfluidic device for cell separation, cell lysis and DNA purification Anal Chim Acta 584 2 237 243 10.1016/j.aca.2006.11.057
5. J Cho S Han E Paek I Cho S Paek 2006 Plastic ELISA-on-a-chip based on sequential cross-flow chromatography Anal Chem 78 3 793 800 10.1021/ac051453v
6. D Cui Z Geng X Chen 2006 A hybrid Silicon-PDMS micropump actuated by PZT bimorph Rare Metal Mater Eng 35 Suppl 3 319 320
7. C Effenhauser G Bruin A Paulus M Ehrat 1997 Integrated capillary electrophoresis on flexible silicone microdevices: analysis of DNA restriction fragments and detection of single DNA molecules on microchips Anal Chem 69 17 3451 3457 10.1021/ac9703919
8. A Hatch A Kamholz K Hawkins M Munson E Schilling B Weigl P Yager 2001 A rapid diffusion immunoassay in a T-sensor Nat Biotechnol 19 5 461 465 10.1038/88135
9. J Higgins S Nasarabadi J Karns D Shelton M Cooper A Gbakima R Koopman 2003 A handheld real time thermal cycler for bacterial pathogen detection Biosens Bioelectron 18 9 1115 1123 10.1016/S0956-5663(02)00252-X
10. M Johnson J Landers 2004 Fundamentals and practice for ultrasensitive laser-induced fluorescence detection in microanalytical systems Electrophoresis 25 21-22 3513 3527 10.1002/elps.200406086
11. H Li J Lin R Su K Uchiyama T Hobo 2004 A compactly integrated laser-induced fluorescence detector for microchip electrophoresis Electrophoresis 25 12 1907 1915 10.1002/elps.200305867
12. Z Liang N Chiem G Ocvirk T Tang K Fluri D Harrison 1996 Microfabrication of a planar absorbance and fluorescence cell for integrated capillary electrophoresis devices Anal Chem 68 6 1040 1046 10.1021/ac950768f
13. C Lin G Lee L Fu S Chen 2004 Integrated optical-fiber capillary electrophoresis microchips with novel spin-on-glass surface modification Biosens Bioelectron 20 1 83 90 10.1016/j.bios.2003.09.011
14. C Liu D Cui H Cai X Chen Z Geng 2006 A rigid poly (dimethylsiloxane) sandwich electrophoresis microchip based on thin-casting method Electrophoresis 27 14 2917 2923 10.1002/elps.200500581
15. C Liu D Cui X Chen 2007 Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection J Chromatogr A 1170 1-2 101 106 10.1016/j.chroma.2007.08.087
16. A Manz J Fettinger E Verpoorte H Lüdi H Widmer D Harrison 1991 Micromachining of monocrystalline silicon and glass for chemical analysis systems A look into next century's technology or just a fashionable craze? Trends Analyt Chem 10 5 144 149 10.1016/0165-9936(91)85116-9
17. K Miyaki Y Guo T Shimosaka T Nakagama H Nakajima K Uchiyama 2005 Fabrication of an integrated PDMS microchip incorporating an LED-induced fluorescence device Anal Bioanal Chem 382 3 810 816 10.1007/s00216-004-3015-1
18. L Novak P Neuzil J Pipper Y Zhang S Lee 2007 An integrated fluorescence detection system for lab-on-a-chip applications Lab Chip 7 1 27 29 10.1039/b611745g
19. D Rosamund J Finkelstein 2006 Insight: Lab on a chip Nature 442 7101 367 418 10.1038/442367a