Evaluation of a quantitative real-time PCR assay to measure HIV-specific mucosal CD8+ T cell responses in the cervix

PLoS ONE

Volumn 5, Issue 10, 2010, Pages

  Chege, Duncan ^a   Chai, Yijie ^a   Huibner, Sanja ^a   McKinnon, Lyle ^a,d,f   Wachihi, Charles ^f   Kimani, Makubo ^f   Jaoko, Walter ^f   Kimani, Joshua ^d,e,f   Blake Ball, T ^d,e,f   Plummer, Francis A ^d,e,f   Kaul, Rupert ^a,b,f   Rebbapragada, Anuradha ^a,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY HEALTH NETWORK   (Canada)

^c PUBLIC HEALTH ONTARIO   (Canada)

^d UNIVERSITY OF MANITOBA   (Canada)

^e PUBLIC HEALTH AGENCY OF CANADA   (Canada)

^f UNIVERSITY OF NAIROBI   (Kenya)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; EPITOPE; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 2; MESSENGER RNA; STAPHYLOCOCCUS ENTEROTOXIN A; SUPERANTIGEN; TUMOR NECROSIS FACTOR ALPHA; CYTOKINE;

ARTICLE; CD8+ T LYMPHOCYTE; CELL COUNT; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOKINE PRODUCTION; EVALUATION; EX VIVO STUDY; GENE EXPRESSION; GENE INDUCTION; GENETIC TRANSCRIPTION; GENITAL SYSTEM; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; IMMUNE RESPONSE; MONONUCLEAR CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UTERINE CERVIX; BIOSYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENETICS; IMMUNOLOGY; METHODOLOGY; MUCOSAL IMMUNITY; POLYMERASE CHAIN REACTION;

CD8-POSITIVE T-LYMPHOCYTES; CERVIX UTERI; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; FEMALE; HIV; HUMANS; IMMUNITY, MUCOSAL; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 78049317278     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0013077     Document Type: Article

References (43)

1. UNAIDS, Organization WH (2009) AIDS epidemic update: November 2009 (UNAIDS/09.36E/JC1700E).
2. Hladik F, McElrath MJ (2008) Setting the stage: host invasion by HIV. Nat Rev Immunol 8: 447-457.
3. Graziosi C, Gantt KR, Vaccarezza M, Demarest JF, Daucher M, et al. (1996) Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection. Proc Natl Acad Sci U S A 93: 4386-4391.
4. Vernazza PL, Eron JJ, Fiscus SA, Cohen MS (1999) Sexual transmission of HIV: infectiousness and prevention. AIDS 13: 155.
5. Demberg T, Robert-Guroff M (2009) Mucosal immunity and protection against HIV/SIV infection: strategies and challenges for vaccine design. Int Rev Immunol 28: 20-48.
6. Gumbi PP, Nkwanyana NN, Bere A, Burgers WA, Gray CM, et al. (2008) Impact of mucosal inflammation on cervical human immunodeficiency virus (HIV-1)-specific CD8 T-cell responses in the female genital tract during chronic HIV infection. J Virol 82: 8529-8536.
7. Kaul R, Plummer FA, Kimani J, Dong T, Kiama P, et al. (2000) HIV-1-specific mucosal CD8+ lymphocyte responses in the cervix of HIV-1-resistant prostitutes in Nairobi. J Immunol 164: 1602-1611.
8. Kaul R, Thottingal P, Kimani J, Kiama P, Waigwa CW, et al. (2003) Quantitative ex vivo analysis of functional virus-specific CD8 T lymphocytes in the blood and genital tract of HIV-infected women. Aids 17: 1139-1144.
9. Musey L, Hu Y, Eckert L, Christensen M, Karchmer T, et al. (1997) HIV-1 induces cytotoxic T lymphocytes in the cervix of infected women. J Exp Med 185: 293-303.
10. Shacklett BL, Cu-Uvin S, Beadle TJ, Pace CA, Fast NM, et al. (2000) Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. Aids 14: 1911-1915.
11. Bere A, Denny L, Hanekom W, Burgers WA, Passmore JA (2010) Comparison of polyclonal expansion methods to improve recovery of cervical cytobrushderived T cells from the female genital tract of HIV-infected women Journal of Immunological Methods In press.
12. Subbramanian RA, Kuroda MJ, Charini WA, Barouch DH, Costantino C, et al. (2003) Magnitude and diversity of cytotoxic-T-lymphocyte responses elicited by multiepitope DNA vaccination in rhesus monkeys. J Virol 77: 10113-10118.
13. Allen TM, Vogel TU, Fuller DH, Mothe BR, Steffen S, et al. (2000) Induction of AIDS virus-specific CTL activity in fresh, unstimulated peripheral blood lymphocytes from rhesus macaques vaccinated with a DNA prime/modified vaccinia virus Ankara boost regimen. J Immunol 164: 4968-4978.
14. Buchbinder SP, Mehrotra DV, Duerr A, Fitzgerald DW, Mogg R, et al. (2008) Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial. Lancet 372: 1881-1893.
15. McEvers K, Elrefaei M, Norris P, Deeks S, Martin J, et al. (2005) Modified anthrax fusion proteins deliver HIV antigens through MHC Class I and II pathways. Vaccine 23: 4128-4135.
16. Girard MP, Bansal GP, Pedroza-Martins L, Dodet B, Mehra V, et al. (2008) Mucosal immunity and HIV/AIDS vaccines. Report of an International Workshop, 28-30 October 2007. Vaccine 26: 3969-3977.
17. Sallusto F, Geginat J, Lanzavecchia A (2004) Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu Rev Immunol 22: 745-763.
18. Betts MR, Nason MC, West SM, De Rosa SC, Migueles SA, et al. (2006) HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood 107: 4781.
19. Listvanova S, Temmerman S, Stordeur P, Verscheure V, Place S, et al. (2003) Optimal kinetics for quantification of antigen-induced cytokines in human peripheral blood mononuclear cells by real-time PCR and by ELISA. J Immunol Methods 281: 27-35.
20. Stordeur P, Poulin LF, Craciun L, Zhou L, Schandené L, et al. (2002) Cytokine mRNA quantification by real-time PCR. Journal of Immunological Methods 259: 55-64.
21. Nicol AF, Nuovo GJ, Wang Y, Grinsztejn B, Tristão A, et al. (2006) In situ detection of SOCS and cytokine expression in the uterine cervix from HIV/HPV coinfected women. Experimental and Molecular Pathology 81: 42-47.
22. Härtel C, Bein G, Müller-Steinhardt M, Klüter H (2001) Ex vivo induction of cytokine mRNA expression in human blood samples. Journal of Immunological Methods 249: 63-71.
23. Kruse N, Moriabadi NF, Toyka KV, Rieckmann P (2001) Characterization of early immunological responses in primary cultures of differentially activated human peripheral mononuclear cells. J Immunol Methods 247: 131-139.
24. Steinau M, Rajeevan MS, Unger ER (2006) DNA and RNA references for qRTPCR assays in exfoliated cervical cells. J Mol Diagn 8: 113-118.
25. Daud II, Scott ME (2008) Validation of reference genes in cervical cell samples from human papillomavirus-infected and -uninfected women for quantitative reverse transcription-PCR assays. Clin Vaccine Immunol 15: 1369-1373.
26. Musey L, Ding Y, Cao J, Lee J, Galloway C, et al. (2003) Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes. J Virol 77: 291-300.
27. Roederer M, Dubs JG, Anderson MT, Raju PA, Herzenberg LA (1995) CD8 naive T cell counts decrease progressively in HIV-infected adults. J Clin Invest 95: 2061-2066.
28. Nkwanyana NN, Gumbi PP, Roberts L, Denny L, Hanekom W, et al. (2009) Impact of human immunodeficiency virus 1 infection and inflammation on the composition and yield of cervical mononuclear cells in the female genital tract. Immunology 128: e746-757.
29. Quayle AJ, Shah M, Cu-Uvin S, Politch JA, Chou C, et al. (2004) Implications of blood contamination for assessment of local cellular immunity in the endocervix. AIDS Res Hum Retroviruses 20: 543-546.
30. Verkooyen RP, Luijendijk A, Huisman WM, Goessens WH, Kluytmans JA, et al. (1996) Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J Clin Microbiol 34: 3072-3074.
31. Toye B, Woods W, Bobrowska M, Ramotar K (1998) Inhibition of PCR in genital and urine specimens submitted for Chlamydia trachomatis testing. J Clin Microbiol 36: 2356-2358.
32. Mygind T, Birkelund S, Birkebaek NH, Ostergaard L, Jensen JS, et al. (2002) Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae. BMC Microbiol 2: 17.
33. Fleischer B (1991) Stimulation of human T cells by microbial 'superantigens'. Immunol Res 10: 349-355.
34. Kaul R, Rowland-Jones SL, Kimani J, Fowke K, Dong T, et al. (2001) New insights into HIV-1 specific cytotoxic T-lymphocyte responses in exposed, persistently seronegative Kenyan sex workers. Immunol Lett 79: 3-13.
35. Proft T, Fraser J (1998) Superantigens: just like peptides only different. J Exp Med 187: 819-821.
36. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6: 279-284.
37. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.
38. Geiss GK, Bumgarner RE, An MC, Agy MB, van't Wout AB, et al. (2000) Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays. Virology 266: 8-16.
39. Watson S, Mercier S, Bye C, Wilkinson J, Cunningham AL, et al. (2007) Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses. Virol J 4: 130.
40. van Wanrooij EJ, Happe H, Hauer AD, de Vos P, Imanishi T, et al. (2005) HIV entry inhibitor TAK-779 attenuates atherogenesis in low-density lipoprotein receptor-deficient mice. Arterioscler Thromb Vasc Biol 25: 2642-2647.
41. Smith KA (1989) The interleukin 2 receptor. Annu Rev Cell Biol 5: 397-425.
42. Kaul R, Thottingal P, Kimani J, Kiama P, Waigwa CW, et al. (2003) Quantitative ex vivo analysis of functional virus-specific CD8 T lymphocytes in the blood and genital tract of HIV-infected women. AIDS 17: 1139.
43. Yun JJ, Heisler LE, Hwang II, Wilkins O, Lau SK, et al. (2006) Genomic DNA functions as a universal external standard in quantitative real-time PCR. Nucleic Acids Res 34: e85.