Improvement of the genomic DNA extraction method with CTAB for cotton leaves;Amélioration de la méthode d’extraction d’ADN au CTAB appliquée aux feuilles de cotonnier

Biotechnology, Agronomy and Society and Environment

Volumn 10, Issue 2, 2006, Pages 73-76

  Benbouza, Halima ^a   Baudoin, Jean Pierre ^a   Mergeai, Guy ^a  

^a Unité de Phytotechnie tropicale et Horticulture   (Belgium)

Author keywords

Activated charcoal; CTAB; DNA extraction; Gossypium hirsutum L.; Microsatellites; Recalcitrant

Indexed keywords

GOSSYPIUM HIRSUTUM;

EID: 78049290494     PISSN: 13706233     EISSN: 17804507     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Benbouza H., Jacquemin JM., Baudoin JP., Mergeai G. (2006). Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol. Agron. Soc. Environ. 10 (2), p. 77–81.
2. Chandellier A. (1995). Structure du génome mitochondrial de l’épicéa commun (Picea abies L. Karst) lors de l’embryogenèse somatique in vitro. Thèse Doct. Sci. agron. Gembloux, Fac. univ. Sci. agron. 240 p.
3. Dabo SM., Mitchel ED., Melcher U. (1993). A method for the isolation of nuclear DNA from cotton (Gossypium) leaves. Ann. Biochem. 210, p. 34–38.
4. Liu S., Saha S., Stelly D., Burr B., Cantrell RG. (2000). Chromosomal assignment of microsatellites loci in Cotton. Amer. Genet. Assoc. 91, p. 326–332.
5. Murray M., Thompson WF. (1980). Rapid isolation of high molecular weight plant DNA. Nucl. Acids. Res. 8, p. 4321–4325.
6. Oakeley EJ., Lazarus CM., McDonald H. (1994). The use of activated charcoal to remove endogenous fl uorescence from tobacco callus extracts. Plant. Mol. Biol. Rep. 12, p. 14–19.
7. Paterson AH., Brubaker CL., Wendel JF. (1993). A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant. Mol. Biol. Rep. 11, p. 122–127.
8. Permingeat HR., Romagnoli MV., Vallejos RH. (1998). A simple method for isolating high yield and quality DNA from (Gossypium hirsutum L.) leaves. Plant. Mol. Biol. Rep. 16, p. 1–6.
9. Vroh Bi I., Harvengt L., Chandelier A., Mergeai G., Du Jardin P. (1996). Improved RAPD amplifi cation of recalcitrant plant DNA by the use of activated charcoal during DNA extraction. Plant. Breed. 115, p. 205–206.
10. Zhang J., Stewart JM. (2001). Economical and rapid method for extracting cotton genomic DNA. J. Cot. Sci. 4, p. 193–201.