Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD

Acta Physiologiae Plantarum

Volumn 32, Issue 3, 2010, Pages 443-452

  Antony Ceasar, S ^a,b   Lenin Maxwell, S ^b   Bhargav Prasad, K ^b   Karthigan, M ^b   Ignacimuthu, Savarimuthu ^a  

^a LOYOLA COLLEGE   (India)

^b St Peter's Engineering College   (India)

Author keywords

Auxin; Cytokinin; Genetic integrity; Induction medium; Multiplication medium; Phenolic compounds

Indexed keywords

BACOPA MONNIERI;

EID: 78049269946     PISSN: 01375881     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11738-009-0419-8     Document Type: Article

References (45)

1. Ali G, Shrivastav PS, Iabal M (2001) Responses of Bacopa monnera cultures to cadmium toxicity. Bull Environ Con Tox 66:342-349
2. Anonoymous (1997) Indian medicinal plants: a sector study. Occasional paper No. 54. Export-Import Bank of India, Quest publications, Bombay, India
3. Capelle S, Mok D, Kirchner S, Mok S (1983) Effects of thidiazuran on (D2-isopentyl) (8-14C) adenosine in callus tissues of phaseolus linatus L. Plant Physiol 73:796-802
4. David JJ (1983) Adventitious root formation 'in vitro' in apple rootstocks (Malus pumila) I. Factors affecting the length of the auxin-sensitive phase in M9. Physiol Plant 5:149-153
5. De Klerk GJ, Vander Kreiken W, De Jong JC (1999) The formation of adventitious roots: new concepts, new possibilities. In Vitro Cell Dev Biol Plant 35:189-199
6. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation. Version II. Plant Mol Biol Rep 1:19-21
7. Dimitrov B, Tashera K, Zagorska N, Evstatiera L (2003) In vitro cultivation of Rhodiola rosea L. Gene Breed 32:3-6
8. Faisal M, Anis M (2005) An efficient in vitro method for mass propagation of Tylophora indicia. Biol Plant 49:257-260
9. Geetha N, Venkatachalam P, Prakash V, Lakshmi Sita G (1998) High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan L.). Curr Sci 75:1036-1041
10. Giridhar P, Gururaj H, Ravishankar G (2005) In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight & Arn., a threatened plant endemic to Southern India. In Vitro Cell Dev Biol Plant 41:77-80
11. González A, Casares TR, Sánchez Rodríguez R (1991) Adventitious root induction in Corylusvellana L. cotyledon. In Vitro Cell Dev Bio Plant 27:125-131
12. Hoque A, Arima S (2002) Overcoming phenolic accumulation during callus induction and in vitro organogenesis in water chestnut (Trapa japonica Flerov.). In Vitro Cell Dev Biol Plant 38:342-346
13. Husain MK, Avis M, Shahzad A (2007) In vitro propagation of Indian kino (Pterocarpus marsupium. Rokb.) using Thidiazuron. In Vitro Cell Dev Biol Plant 43:59-64
14. Ibrahim R, Debergh PC (2001) Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida L.). Sci Hortic 88:41-57
15. Iram S, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.), a medicinal plant. Acta Phys Plant 29:233-238
16. Jayanthi M, Mandal PK (2001) Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. F. Merrill.). In Vitro Cell Dev Biol Plant 37:576-580
17. Kanita A, Kothari SL (2002) High efficiency adventious shoot bud formation and plant regeneration from leaf explants of Dianthus chinesis L. Sci Hortic 90:205-212
18. Kawiak AA, Lojkowska E (2004) Application of RAPD in the determination of genetic fidelity in micropropagated drosera plantlets in vitro cell. Dev Biol Plant 40:592-595
19. Konan NK, Sangwan RS, Sangwan-Norreel BS (1994) Efficient in vitro shoot-regeneration systems in Cassava (Manihot esculenta Crantz). Plant Breed 113:227-236
20. Liu HJ, Xu YAN, Liu Y-J, Liu C-Z (2006) Plant regeneration from leaf explants of Rhodiola fastigiata. In Vitro Cell Dev Biol Plant 42:345-347
21. Magioli C, Rocha AP, Oiveira DE (2004) Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep 7:661-663
22. 0-1, 2, 3-thiadiazol-5-urea (thidiazuron). Phytochemistry 21:1509-1511
23. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol Plant 15:497-573
24. Prakash S, Elangomathavan R, Seshadri S, Kathiravan K, Ignacimuthu S (2004) Efficient regeneration of Curcuma amada Roxb plantlets from rhizome and leaf sheath explants. Plant Cell Tissue Organ Cult 78:159-165
25. Rahman LU, Verma PC, Singh D, Gupta MM, Banerjee S (2002) Bacosides production by suspension cultures of Bacopa monnieri (L). Biotechnol Lett 24:1427-1429
26. Ramana M, Gosukonda, Ananta Porobodessai, Essie Blay, Channapatna S, Prakasha Curt M, Peterson (1995) Thidiazuron induced adventitious shoot regeneration of sweet potato (Ipomoea batatas). In Vitro Cell Dev Biol Plant 31:65-71
27. Rout GR, Das (2002) An assessment of genetic integrity of micropropagated plants of Plumbago zeylanica by RAPD markers. Biol Plant 45:27-32
28. Rout GR, Das P, Goel S, Raina SN (1998) Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers. Bot Bull Acad Sin 39:23-27
29. Selvi DN, George L, Eapen S (2002) Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell Tissue Organ Cult 68:143-151
30. Sen J, Sen S, (1995) Two-step bud culture technique for a high frequency regeneration of Gladiolus corms. Sci Hortic 64:133-138
31. Shrivastav N, Ranjani M (1999) Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennel. Plant Cell Rep 18:919-923
32. Shu QY, Liu GS, Qi DM, Chu CC, Liu J, Li HJ (2003) An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust. Plant Cell Rep 22:175-180
33. Shukla OP, Pubey S, Ravi VN (2007) Preferential accumulation of cadmium and chromium: toxicity in Bacopa monnieri. L. under mixed metal treatments. Bull Environ Contam Toxicol 78:252-257
34. Singh HK, Rastogi RP, Sriman RC, Dhawan BN (1988) Effect of bacoside A and B on avoidance response in rats. Phytother Res 2:70-75
35. Skala E, Wysokinska H (2004) In vitro regeneration of Salvia nemorosa L. from shoot tips and leaf explants. In Vitro Cell Dev Biol Plant 40:596-602
36. Stough C, Lloyd J, Clarke J, Downey LA, Hutchison CW, Rodgers T, Nathan PJ (2001) The chronic effects of an extract Bacopa monniera (Brahimi) on cognitive function in healthy human subjects. Psychopharmacology 156:481-484
37. Tang W, Guo Z (2001) In vitro propagation of loblolly pine via direct somatic organogenesis from mature cotyledons and hypocotyls. Plant Growth Regul 33:25-31
38. Thomas JC, Katterman FR (1986) Cytokinin activity induced by thidiazuran. Plant Physiol 81:681-683
39. Tiwari V, Deo Singh B, Nath Tiwari K (1998) Shoot regeneration and Somatic embryogenesis from different explants of Brahmi (Bacopa monniera (L.) Wettst.). Plant Cell Rep 17:538-543
40. Tiwari V, Tewari KN, Singh BD (2000) Suitability of liquid cultures for in vitro multiplication of Bacopa Monniera (L.) Wettst. Phytomorphology 50:337-342
41. Tiwari V, Tewari KN, Singh BD (2001) Comparative studies of cytokinin on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult 66:9-16
42. Tiwari V, Tewari KN, Singh BD (2006) Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin. Plant Cell Rep 25:629-635
43. Venkatachalam LE, Sreedhar RV, Bhagyalakshmi N (2007) Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers. Plant Growth Regul 51:193-205
44. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535
45. Zanol GC, Fortes GR, de L, Campos AD, Centellas AQ, da Silva JB (1998) In vitro rooting and peroxidase activity of apple rootstock treated with indole butyric acid and phloroglucinol. Res Brasil Fisiol Veg 10:65-68