Measuring plasma membrane protein endocytic rates by reversible biotinylation

Journal of Visualized Experiments

Volumn , Issue 34, 2010, Pages

  Gabriel, Luke ^a   Stevens, Zachary ^a   Melikian, Haley ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Biotinylation; Cell biology; Endocytosis; Membrane trafficking

Indexed keywords

EID: 78049239374     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1669     Document Type: Article

References (4)

1. Holton, K. L., Loder, M. K. & Melikian, H. E. Nonclassical, distinct endocytic signals dictate constitutive and PKC-regulated neurotransmitter transporter internalization. Nat Neurosci 8, 881-8 (2005).
2. Boudanova, E., Navaroli, D.M., Melikian, H.E. Amphetamine-induced decreases in dopamine transporter surface expression are protein kinase C-independent. Neuropharmacology 54, 605-612 (2008).
3. Boudanova, E., Navaroli, D. M., Stevens, Z. & Melikian, H. E. Dopamine transporter endocytic determinants: carboxy terminal residues critical for basal and PKC-stimulated internalization. Mol Cell Neurosci 39, 211-7 (2008).
4. Loder, M. K. & Melikian, H. E. The dopamine transporter constitutively internalizes and recycles in a protein kinase C-regulated manner in stably transfected PC12 cell lines. J Biol Chem 278, 22168-74 (2003).