The three proline residues (P25, P242, and P434) of Agrobacterium CP4 5-enolpyruvylshikimate-3-phosphate synthase are crucial for the enzyme activity

Plant Biotechnology Reports

Volumn 4, Issue 4, 2010, Pages 329-334

  Kang, Kyungsu ^a,b   Jin, Yong Mei ^a   Jeon, Hyesung ^a   Park, Sang Ryoung ^a   Song, Dae geun ^b   Lee, Joo Young ^b   Pan, Cheol Ho ^b   Kim, Minkyun ^a  

^a Seoul National University   (South Korea)

^b Korea Institute of Science and Technology   (South Korea)

Author keywords

5 Enolpyruvylshikimate 3 phosphate synthase (EPSPS); Agrobacterium sp. strain CP4; Glyphosate; Herbicide resistance; Proline residue

Indexed keywords

AGROBACTERIUM; AGROBACTERIUM SP.; PROKARYOTA; RHIZOBIUM;

EID: 77958453863     PISSN: 18635466     EISSN: 18635474     Source Type: Journal    
DOI: 10.1007/s11816-010-0150-3     Document Type: Article

References (21)

1. Balbach J, Schmid FX (2000) Proline isomerization and its catalysis in protein folding. In: Pain RH (ed) Mechanisms of protein folding, 2nd edn. Oxford University Press, UK.
2. Boocock MR, Coggins JR (1983) Kinetics of 5-enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate. FEBS Lett 154: 127-133.
3. Bull HB, Breese K (1974) Surface tension of amino acid solutions: a hydrophobicity scale of the amino acid residues. Arch Biochem Biophys 161: 665-670.
4. Duke SO, Powles SB (2008) Glyphosate: a once-in-a-century herbicide. Pest Manag Sci 64: 319-325.
5. Eisenberg D, McLachlan AD (1986) Solvation energy in protein folding and binding. Nature 319: 199-203.
6. Eschenburg SM, Healy ML, Priestman MA, Lushington GH, Schönbrunn E (2002) How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. Planta 216: 129-135.
7. Funke T, Han H, Healy-Fried ML, Fisher M, Schönbrunn E (2006) Molecular basis for the herbicide resistance of Roundup Ready crops. Proc Natl Acad Sci USA 103: 13010-13015.
8. Garcia-Effron G, Katiyar SK, Park S, Edlind TD, Perlin DS (2008) A naturally occurring proline-to-alanine amino acid change in Fks1p in Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis accounts for reduced echinocandin susceptibility. Antimicrob Agents Chemother 52: 2305-2312.
9. Herrmann KM (1995) The shikimate pathway: early steps in the biosynthesis of aromatic compounds. Plant Cell 7: 907-919.
10. Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59.
11. Kang K, Kim M (2006a) Expression of the glyphosate resistant gene, cp4-epsps, through plastid transformation in rice (Oryza sativa L.). J Plant Biotechnol 33: 75-84.
12. Kang K, Kim M (2006b) Single amino acid changes in the 5-enolpyruvylshikimate-3-phosphate synthase from Agrobacterium sp. strain CP4 and Synechocystis sp. PCC6803 alter enzyme activity and glyphosate sensitivity. Agric Chem Biotechnol 49: 65-68.
13. Kelly RF, Richards FM (1987) Replacement of proline-76 with alanine eliminates the slowest kinetic phase in thioredoxin folding. Biochem 26: 6765-6774.
14. Kishore GM, Shah DM (1988) Amino acid biosynthesis inhibitors as herbicides. Annu Rev Biochem 57: 627-663.
15. Lanzetta PA, Alvarez LJ, Reinach PS, Candia OA (1979) An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem 100: 95-97.
16. Malik J, Barry G, Kishore G (1989) The herbicide glyphosate. Biofactors 2: 17-25.
17. Padgette SR, Re DB, Barry GF, Eichholtz DE, Delannay X, Fuchs RL, Kishore GM, Fraley RT (1996) New weed control opportunities: development of soybeans with a Roundup Ready gene. In: Duck SO (ed) Herbicide-resistant crops: agricultural, economic, environmental, regulatory and technological aspects. CRC Press, Boca Raton, USA, pp 53-84.
18. Park H, Hilsenbeck JL, Kim HJ, Shuttleworth WA, Park YH, Evans JN, Kang K (2003) Structural studies of Streptococcus pneumoniae EPSP synthase in unliganded state, tetrahedral intermediate-bound state and S3P-GLP-bound state. Mol Microbiol 51: 963-971.
19. Schönbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W (2001) Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikikate 3-phosphate synthase in atomic detail. Proc Natl Acad Sci USA 98: 1376-1380.
20. Schulz GE, Schirmer RH (1979) Principle of protein structure. Springer, New York.
21. Steinrucken HC, Amerhein N (1984) 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumonia 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine]. Eur J Biochem 143: 351-357.