Isolation and identification of Perkinsus olseni from feces and marine sediment using immunological and molecular techniques

Journal of Invertebrate Pathology

Volumn 105, Issue 3, 2010, Pages 261-269

  Park, Kyung Il ^a   Yang, Hyun Sung ^b   Kang, Hyun Sil ^b   Cho, Moonjae ^b   Park, Kwang Jae ^c   Choi, Kwang Sik ^b  

^a Kunsan National University   (South Korea)

^b Jeju National University   (South Korea)

^c National Fisheries Research and Development Institute (NFRDI)   (South Korea)

Author keywords

Fecal discharge; Korea; Perkinsus olseni; Polyclonal antibody; Ruditapes philippinarum; Transmission

Indexed keywords

PROTOZOAL PROTEIN; PROTOZOON ANTIBODY;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BIVALVE; EUKARYOTE; FECES; IMMUNOLOGY; PARASITOLOGY; PHYSIOLOGY; SEDIMENT; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIBODY SPECIFICITY; BIVALVIA; BLOTTING, WESTERN; EUKARYOTA; FECES; GEOLOGIC SEDIMENTS; PROTOZOAN PROTEINS;

BIVALVIA; ORYCTOLAGUS CUNICULUS; PERKINSUS OLSENI; PROTOZOA; VENERUPIS (RUDITAPES) PHILIPPINARUM;

EID: 77958166053     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2010.07.006     Document Type: Article

References (49)

1. Ahn K.-J., Kim K.H. Effect of temperature and salinity on the in vitro zoosporulation of Perkinsus sp. in Manila clams Ruditapes philippinarum. Dis. Aquat. Org. 2001, 48:43-46.
2. Andrews J.D. Epizootiology of the disease caused by the oyster pathogen Perkinsus marinus and its effects on the oyster industry. Am. Fish. Soc. Spec. Publ. 1988, 18:47-63.
3. Andrews J.D., Hewatt W.G. Oyster mortality studies in Virginia. II. The fungus disease caused by Dermocystidium marinum in oysters of Chesapeake Bay. Ecol. Monogr. 1957, 27:1-26.
4. Audemard C.L., Calvo M.R., Paynter K.T., Reece K.S., Burreson E.M. Real-time PCR investigation of parasite ecology: in situ determination of oyster parasite Perkinsus marinas transmission dynamics in lower Chesapeake Bay. Parasitology 2006, 132:827-842.
5. Auzoux-Bordenave S., Vigario A.M., Ruano F., Domart-Coulon I., Doumenc D. In vitro sporulation of the clam pathogen Perkinsus atlanticus (Apicomplexa, Perkinsea) under various environmental conditions. J. Shellfish Res. 1995, 14:469-475.
6. Bondad-Reantaso, M.G., McGladdery, S.E., Subasinghe, R.P., 2001. Asia Diagnostic Guide to Aquatic Animal Diseases. FAO Fisheries Technical Paper No, 402, Supplement 2. Rome, FAO. 240 pp.
7. Bushek D., Allen S.K., Alcox K.A., Gustason R., Ford S.E. Response of Crassostrea virginica to in vitro-cultured Perkinsus marinus: preliminary comparison of three inoculation methods. J. Shellfish Res. 1997, 16:479-485.
8. Bushek D., Dungan C.F., Lewitus A.J. Serological affinities of the oyster pathogen Perkinsus marinus (Apicomplexa) with some dinoflagellates (Dinophyceae). J. Eurkaryot. Microbiol. 2002, 49:11-16.
9. Bushek D., Ford S.E., Chintala M.M. Comparison of in vitro-cultured and wild type Perkinsus marinus. III. Fecal elimination and its role in transmission. Dis. Aquat. Org. 2002, 51:217-225.
10. Choi K.-S., Park K.-I. Report on the occurrence of Perkinsus sp. in the Manila Clam, Ruditapes philippinarum in Korea. Korean J. Aquacult. 1997, 10:227-237.
11. Choi K.-S., Wilson E.A., Lewis D.H., Powell E.N., Ray S.M. The energetic cost of Perkinsus marinus parasitism in oysters: quantification of the thioglycollate method. J. Shellfish Res. 1989, 8:125-131.
12. Choi K.-S., Lewis D.H., Powell E.N., Frelier P.F., Ray S.M. A polyclonal antibody developed from Perkinsus marinus hypnospores fails to cross-react with other life stages of P. marinus present in oyster Crassostrea virginica. J. Shellfish Res. 1991, 10:411-415.
13. Dungan C.F., Reece K.S. In vitro propagation of two Perkinsus spp. Parasites from Japanese Manila clams Venerupis philippinarum and description of Perkinsus honshuensis n. sp. J. Eukaryot. Microbiol. 2006, 53:316-326.
14. Dungan C.F., Roberson B.S. Binding specificities of monoclonal and polyclonal antibodies to the protozoan oyster pathogen Perkinsus marinus. Dis. Aquat. Org. 1993, 15:9-22.
15. Fuchs, S., Sela, M., 1979. Immunoadsorbents. In: Weir, D.M. (Ed.), Handbook of Experimental Immunology, vol. 1. Immunochenistry. Blackwell Scientific Publications, Philadelphia, USA, pp. 10.1-10.6.
16. Hamaguchi M.N., Suzuki N., Usuki H., Ishioka H. Perkinsus protozoan infection in short-necked clam Tapes (=Ruditapes) philippinarum in Japan. Fish Pathol. 1998, 33:473-480.
17. Liang, Y., Liang, B., 2007. Spatial distribution of the protozoan parasite Perkinsus sp. found in the Manila clams Ruditapes philippinarum in China. In: World Aquaculture 2007 Abstract Book. San Antonio Convention Center, San Antonio, Texas, pp. 533.
18. Liu Q.Q., Allam B., Collier J.L. Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria). Appl. Environ. Microbiol. 2009, 75:4913-4918.
19. Mackin J.G. Oyster disease caused by Dermocystidium marinum and other microorganisms in Louisiana. Public. Ins. Mar. Sci. Univ. Texas 1962, 7:132-229.
20. Montes F., Durfort M., García-Valero J. Characterization and localization of an Mr 225kDa polypeptide specifically involved in the defense mechanisms of the clam Tapes semidecussatus. Cell Tissue Res. 1995, 280:27-37.
21. Montes F., Durfort M., García-Valero J. Ultrastructural localization of antigenic determinants conserved during Perkinsus atlanticus trophozoites to prezoosporangium differentiation. Dis. Aquat. Org. 2005, 66:33-40.
22. Moon S.-G., Choi C.-M. A list of important species and distribution of marine phytoplankton in Korea. J. Environ. Sci. 2003, 7:725-733. (in Korean with English abstract).
23. NFRDI, 2001. Development of Optimal Technology for Sustaining Production in Shellfish Farm. Ministry of Maritime Affairs and Fisheries, p. 930 (in Korean with English abstract).
24. NFRDI, 2007. Studies on the Management of the Tidal Flat Aquaculture Animals. 2007 Annual Report of National Fisheries Research and Development Institute (NFRDI) of Korea, p. 113 (in Korean with English abstract).
25. Office International des Epizooties, 2004. http://www.oie.int/eng/maladies/en_classification2007.htm?e1d7.
26. Ordas M.C., Figueras A. In vitro culture of Perkinsus atlanticus, a parasite of the carpet shell clam Ruditapes decussates. Dis. Aquat. Org. 1998, 33:129-136.
27. Ottinger C.A., Lewis T.D., Shapiro D.A., Faisal M., Kaattari S.L. Detection of Perkinsus marinus extracellar proteins in tissues of the eastern oyster Crassostrea virginica: potential use in diagnostic assays. J. Aquat. Anim. Health 2001, 13:133-141.
28. Park, K.-I., 2005. Effects of the Protozoan Parasite Pekinsus olseni (atlanticus) on the Manila clam Ruditapes philippinarum in Korea. Ph.D. Dissertation. Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
29. Park K.-I., Choi K.-S. Spatial distribution and infection intensity of the protozoan parasite Perkinsus sp. In the Manila clam Ruditapes philippinarum in Korea. Aqussaculture 2001, 203:9-22.
30. Park K.-I., Choi K.-S. Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia): I. Quantifying eggs. Aquaculture 2004, 241:667-687.
31. Park K.-I., Park Y.-M., Lee J., Choi K.-S. Development of PCR assay for detection of the protozoan parasite Perkinsus. Korean J. Environ. Biol. 2002, 20:109-117. (in Korean with English abstract).
32. Park K.-I., Park J.-K., Lee J., Choi K.-S. Use of DNA sequences to determine the identity of Perkinsus sp. found in Manila clam, Ruditapes philippinarum in Korean water. Dis. Aquat. Org. 2005, 66:255-263.
33. Park K.-I., Figueras A., Choi K.-S. Application of enzyme-linked immunosorbent assay (ELISA) for the study of reproduction in the Manila clam Ruditapes philippinarum: (Mollusca: Bivalvia): II. Impacts of Perkinsus olseni on clam reproduction. Aquaculture 2006, 251:182-191.
34. Park K.-I., Tsutsumi H., Hong J.-S., Choi K.-S. Pathology survey of the short-neck clam Ruditapes philippinarum occurring on sandy tidal flats along the coast of Ariake Bay, Kyushu. Japan. J. Invertebr. Pathol. 2008, 99:212-219.
35. Perkins F.O. Fine structure of zoospores from Labyrinthomyxa sp. parasitizing the clam Macoma balthica. Chesapeake Sci. 1968, 9:198-208.
36. Perkins F.O. The structure of Perkinsus marinus (Mackin, Owen, and Collier, 1950) Levine, 1978, with comments on the taxonomy and phylogeny of Perkinsus sp. J. Shellfish Res. 1996, 15:67-87.
37. Ragone Calvo L.M., Dungan C.F., Roberson B.S., Burreson E.M. Systematic evaluation of factors controlling Perkinsus marinus transmission dynamics in lower Chesapeake Bay. Dis. Aquat. Org. 2003, 56:75-86.
38. Ray S.M. A culture technique for the diagnosis of infectioin with Dermocystidium marinum Mackin, Owen, and Collier in oysters. Science 1952, 116:360-361.
39. Ray S.M. A review of the culture method for detecting Dermocystidium marinum, with suggested modifications and precautions. Proc. Natl. Shellfish Assoc. 1966, 54:55-69.
40. Ray, S.M., Mackin, J.G., 1954. Studies on transmission and pathogenicity of Dermocystidium marinum. Texas A&M Research Foundation Project 23. Technical Report 1-11.
41. Romestand B., Torreilles J., Roch P. Production of monoclonal antibodies against the Protozoa, Perkinsus marinus: estimation of parasite multification in vitro. Aquat. Living Resour. 2001, 14:351-357.
42. Scanlon C.H., Ragone Calvo L.M., Burreson E.M. The potential for transmission of Perkinsus marinus by fecal matter from the eastern oyster, Crassostrea virginica. J. Shellfish Res. 1997, 16:332.
43. Takahashi M., Yoshinaga T., Waki T., Shimokawa J., Ogawa K. Development of a PCR-RFLP method for differentiation of Perkinsus olseni and P. honshuensis in the Manila clam Ruditapes philippinarum. Fish Pathol. 2009, 44:185-188.
44. Uddin M.-J., Yang H.-S., Choi K.-S., Kim H.-J., Hong J.-S., Cho M. Seasonal changes in Perkinsus olseni infection and gametogenesis in Manila clam, Ruditapes philippinarum, from Seonjaedo Island in Incheon, off the west coast of Korea. J. World Aquacul. Soc. 2010, 41:93-101.
45. Valiulis G.A., Mackin J.G. Formation of sporangia and zoospores by Labyrinthomyxa sp. parasitic in the clam Macoma balthica. J. Invertebr. Pathol. 1969, 14:268-270.
46. Villalba A., Reece K.S., Camino M.C., Casas S.M., Figueras A. Perkinsosis in molluscs: a review. Aquat. Living Resour. 2004, 17:411-432.
47. Villalba A., Casas S.M., Lopez C., Carballa M.J. Study of Perkinsosis in the carpet shell clam Tapes decussatus in Galicia (NW Spain). II. Temporal pattern of disease dynamics and association with clam mortality. Dis. Aquat. Org. 2005, 65:257-267.
48. White M.E., Powell E.N., Ray S.M., Wilson E.A. Host-to-host transmission of Perkinsus marinus in oyster (Crassostrea virginica) populations by the ectoparasitic snail Boonea impressa (Pyramidellidae). J. Shellfish Res. 1987, 6:1-5.
49. Wilson E.A., Powell E.N., Ray S.M. The effect of the ectoparasitic Pyramidellid snail, Boonea impressa, on the growth and health of oysters, Crassostrea virginica, under field conditions. Fish. Bull. 1988, 86:553-566.