Functional expression and magnetic nanoparticle-based Immobilization of a protein-engineered marine fish epoxide hydrolase of Mugil cephalus for enantioselective hydrolysis of racemic styrene oxide

Biotechnology Letters

Volumn 32, Issue 11, 2010, Pages 1685-1691

  Choi, Sung Hee ^a   Kim, Hee Sook ^a   Lee, In Su ^b   Lee, Eun Yeol ^b  

^a Kyungsung University   (South Korea)

^b Kyung Hee University   (South Korea)

Author keywords

Chaperone; Chiral epoxides; Epoxide hydrolase; Immobilization; Mugil cephalus

Indexed keywords

CHAPERONE; CHIRAL EPOXIDES; EPOXIDE HYDROLASES; IMMOBILIZATION; MUGIL CEPHALUS;

ESCHERICHIA COLI; GENES; MARINE ENGINEERING; NANOPARTICLES; SILICA; STYRENE;

NANOMAGNETICS;

EPOXIDE; EPOXIDE HYDROLASE; HYBRID PROTEIN; IMMOBILIZED ENZYME; MAGNETITE NANOPARTICLE; MUTANT PROTEIN; STYRENE OXIDE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; MICROSOME; PLASMID; POINT MUTATION; PROTEIN BINDING; SMEGMAMORPHA;

ANIMALS; CHROMATOGRAPHY, AFFINITY; ENZYMES, IMMOBILIZED; EPOXIDE HYDROLASES; EPOXY COMPOUNDS; ESCHERICHIA COLI; GENE EXPRESSION; HYDROLYSIS; MAGNETITE NANOPARTICLES; MICROSOMES; MUTANT PROTEINS; PLASMIDS; POINT MUTATION; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SMEGMAMORPHA;

ESCHERICHIA COLI; MUGIL CEPHALUS;

EID: 77957967372     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0335-4     Document Type: Article

References (14)

1. Brady D, Jordaan J (2009) Advances in enzyme immobilization. Biotechnol Lett 31: 1639-1650.
2. Choi SH, Kim HS, Lee EY (2009) Comparative homology modeling-inspired protein engineering for improvement of catalytic activity of Mugil cephalus epoxide hydrolase. Biotechnol Lett 31: 1617-1624.
3. de Vries EJ, Janssen DB (2003) Biocatalytic conversion of epoxides. Curr Opin Biotechnol 14: 414-420.
4. Fink AL (1999) Chaperone-mediated protein folding. Physiol Rev 79: 425-449.
5. Hwang S, Choi CY, Lee EY (2010) Bio- and chemo-catalytic preparation of chiral epoxides. J Ind Eng Chem 16: 1-6.
6. Kasai N, Suzuki T, Furukawa Y (1998) Chiral C3 epoxides and halohydrins: Their preparation and synthetic application. J Mol Catal B: Enzym 4: 237-252.
7. Kim J, Grate JW, Wang P (2006) Nanostructures for enzyme stabilization. Chem Eng Sci 61: 1017-1026.
8. 2+: agent to bind and separate histidine-tagged proteins. Chem Comm 2008: 709-711.
9. Lee SJ, Kim HS, Kim SJ, Park S, Kim BJ, Shuler ML, Lee EY (2007) Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus. Biotechnol Lett 29: 237-246.
10. 4/silica/NiO superstructures and their application as magnetically separable high-performance biocatalysts. Chem Comm 25: 3780-3782.
11. Sørensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115: 113-128.
12. Steinreiber A, Faber K (2001) Microbial epoxide hydrolases for preparative biotransformations. Curr Opin Biotechnol 12: 552-558.
13. Tran TAT, Struck DK, Young R (2005) Periplasmic domains define holin-antiholin interactions in T4 lysis inhibition. J Bacteriol 187: 6631-6640.
14. Widersten M, Gurell A, Lindberg D (2010) Structure-function relationships of epoxide hydrolases and their potential use in biocatalysis. Biochim Biophys Acta 1800: 316-326.