Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Biotechnology Letters

Volumn 32, Issue 11, 2010, Pages 1587-1592

  Zhang, Xiaowei ^a   Garcia, Isabel Fernandez ^a   Baldi, Lucia ^a   Hacker, David L ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

CHO cells; Hyperosmolarity; Orbital shaken bioreactor; Polyethylenimine; Transfection

Indexed keywords

CHO CELL; HYPEROSMOLARITY; ORBITAL SHAKEN BIOREACTOR; POLYETHYLENIMINES; TRANSFECTION;

BATCH CELL CULTURE; BIOREACTORS; GROWTH KINETICS; SODIUM CHLORIDE;

CELL CULTURE;

RECOMBINANT PROTEIN; SODIUM CHLORIDE;

ANIMAL; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; HAMSTER; METABOLISM; METHODOLOGY; OSMOTIC PRESSURE;

ANIMALS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; OSMOTIC PRESSURE; RECOMBINANT PROTEINS; SALINE SOLUTION, HYPERTONIC;

BACTERIA (MICROORGANISMS); CRICETULUS GRISEUS;

EID: 77957962291     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0331-8     Document Type: Article

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