Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A β-lactamase chimera

Biomolecular NMR Assignments

Volumn 4, Issue 2, 2010, Pages 127-130

  Morin, Sébastien ^a   Clouthier, Christopher M ^a,b   Gobeil, Sophie ^a,b   Pelletier, Joelle N ^a,b   Gagné, Stéphane M ^a  

^a UNIVERSITÉ LAVAL   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Antibiotic resistance; Chimera; Class A lactamase; PSE 4; TEM 1

Indexed keywords

BETA LACTAMASE; BETA LACTAMASE PSE 4; BETA-LACTAMASE PSE-4; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; NUCLEAR MAGNETIC RESONANCE; PROTEIN ENGINEERING;

BETA-LACTAMASES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 77957959129     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-010-9227-8     Document Type: Article

References (21)

1. RP Ambler AF Coulson JM Frere JM Ghuysen B Joris M Forsman RC Levesque G Tiraby SG Waley 1991 A standard numbering scheme for the class A beta-lactamases Biochem J 276 Pt 1 269 270
2. C Bebrone C Moali F Mahy S Rival JD Docquier GM Rossolini J Fastrez RF Pratt JM Frere M Galleni 2001 CENTA as a chromogenic substrate for studying beta-lactamases Antimicrob Agents Chemother 45 1868 1871 10.1128/AAC.45.6.1868- 1871.2001 (Pubitemid 32466362)
3. F Delaglio S Grzesiek GW Vuister G Zhu J Pfeifer A Bax 1995 NMRPipe: a multidimensional spectral processing system based on UNIX pipes J Biomol NMR 6 277 293 10.1007/BF00197809
4. DA Drummond JJ Silberg MM Meyer CO Wilke FH Arnold 2005 On the conservative nature of intragenic recombination Proc Natl Acad Sci USA 102 5380 5385 10.1073/pnas.0500729102 2005PNAS..102.5380D (Pubitemid 40530266)
5. O Fisette S Morin P-Y Savard P Lagüe SM Gagné 2010 TEM-1 backbone dynamics-insights from combined molecular dynamics and NMR Biophys J 98 637 645 10.1016/j.bpj.2009.08.061
6. W Gronwald RF Boyko FD Sonnichsen DS Wishart BD Sykes 1997 ORB, a homology-based program for the prediction of protein NMR chemical shifts J Biomol NMR 10 165 179 10.1023/A:1018389332160
7. C Jelsch L Mourey JM Masson JP Samama 1993 Crystal structure of Escherichia coli TEM1 beta-lactamase at 1.8 A resolution Proteins 16 364 383 10.1002/prot.340160406
8. BA Johnson RA Blevins 1994 NMRView-a computer program for the visualization and analysis of NMR data J Biomol NMR 4 603 614 10.1007/BF00404272
9. RN Jones HW Wilson WJ Novick Jr AL Barry C Thornsberry 1982 In vitro evaluation of CENTA, a new beta-lactamase-susceptible chromogenic cephalosporin reagent J Clin Microbiol 15 954 958
10. W Kabsch C Sander 1983 Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features Biopolymers 22 2577 2637 10.1002/bip.360221211
11. D Lim F Sanschagrin L Passmore L De Castro RC Levesque NCJ Strynadka 2001 Insights into the molecular basis for the carbenicillinase activity of PSE-4 beta-lactamase from crystallographic and kinetic studies Biochemistry 40 395 402 10.1021/bi001653v (Pubitemid 32063238)
12. A Matagne A Dubus M Galleni JM Frère 1999 The beta-lactamase cycle: a tale of selective pressure and bacterial ingenuity [Review] Nat Prod Rep 16 1 19 10.1039/a705983c (Pubitemid 29092067)
13. MM Meyer JJ Silberg CA Voigt JB Endelman SL Mayo ZG Wang FH Arnold 2003 Library analysis of SCHEMA-guided protein recombination Protein Sci 12 1686 1693 10.1110/ps.0306603 (Pubitemid 36910049)
14. S Morin SM Gagné 2009 NMR dynamics of PSE-4 beta-lactamase: an interplay of ps-ns order and mus-ms motions in the active site Biophys J 96 4681 4691 10.1016/j.bpj.2009.02.068
15. S Morin RC Levesque SM Gagné 2006 H-1, C-13, and N-15 backbone resonance assignments for PSE-4, a 29.5 kDa class A beta-lactamase from Pseudomonas aeruginosa J Biomol NMR 36 11 10.1007/s10858-005-5343-7
16. PY Savard SM Gagné 2006 Backbone dynamics of TEM-1 determined by NMR: evidence for a highly ordered protein Biochemistry 45 11414 11424 10.1021/bi060414q (Pubitemid 44453989)
17. PY Savard A Sosa-Peinado RC Levesque MW Makinen SM Gagné 2004 Letter to the Editor: H-1, C-13 and N-15 backbone resonance assignments for TEM-1, a 28.9 kDa beta-lactamase from E. coli J Biomol NMR 29 433 434 10.1023/B:JNMR.0000032503.96942.68 (Pubitemid 38864854)
18. D Sharma K Rajarathnam 2000 13C NMR chemical shifts can predict disulfide bond formation J Biomol NMR 18 165 171 10.1023/A:1008398416292
19. CM Slupsky RF Boyko VK Booth BD Sykes 2003 Smartnotebook: a semi-automated approach to protein sequential NMR resonance assignments J Biomol NMR 27 313 321 10.1023/A:1025870122182 (Pubitemid 37322105)
20. CA Voigt C Martinez ZG Wang SL Mayo FH Arnold 2002 Protein building blocks preserved by recombination Nat Struct Biol 9 553 558
21. DS Wishart BD Sykes 1994 The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data J Biomol NMR 4 171 180 10.1007/BF00175245