Identification of Critical Residues for the Tight Binding of Both Correct and Incorrect Nucleotides to Human DNA Polymerase λ

Journal of Molecular Biology

Volumn 403, Issue 4, 2010, Pages 505-515

  Brown, Jessica A ^a   Pack, Lindsey R ^a   Sherrer, Shanen M ^a   Kshetry, Ajay K ^b   Newmister, Sean A ^a   Fowler, Jason D ^a   Taylor, John Stephen ^a   Suo, Zucai ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

DNA polymerase fidelity; Nonnatural nucleotide analogs; Nucleotide binding; Pre steady state kinetics; X family DNA polymerase

Indexed keywords

DNA DIRECTED DNA POLYMERASE BETA; DNA DIRECTED DNA POLYMERASE GAMMA; NUCLEOTIDE; DEOXYRIBONUCLEOTIDE; DNA; DNA POLYMERASE BETA2; MUTANT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BINDING AFFINITY; CHEMICAL REACTION KINETICS; DNA BINDING; ENZYME BINDING; HUMAN; HYDROGEN BOND; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN INTERACTION; ALLOSTERISM; AMINO ACID SUBSTITUTION; BASE PAIRING; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENETICS; IN VITRO STUDY; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; SITE DIRECTED MUTAGENESIS;

ALLOSTERIC REGULATION; AMINO ACID SUBSTITUTION; BASE PAIRING; BASE SEQUENCE; CATALYTIC DOMAIN; DEOXYRIBONUCLEOTIDES; DNA; DNA POLYMERASE BETA; HUMANS; HYDROGEN BONDING; IN VITRO TECHNIQUES; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 77957917034     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.09.014     Document Type: Article

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