NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions

Plant Biotechnology Journal

Volumn 8, Issue 6, 2010, Pages 678-690

  Portieles, Roxana ^a   Ayra, Camilo ^a   Gonzalez, Ernesto ^a   Gallo, Araiz ^a   Rodriguez, Raisa ^a   Chacón, Osmany ^b   López, Yunior ^a   Rodriguez, Mayra ^a   Castillo, Juan ^c   Pujol, Merardo ^a   Enriquez, Gil ^a   Borroto, Carlos ^a   Trujillo, Luis ^a   Thomma, Bart P H J ^d   Borrás Hidalgo, Orlando ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b Tobacco Research Institute   (Cuba)

^c Instituto Nacional de Ciencias Agricolas Habana   (Cuba)

^d WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Disease control; Plant defensin; Recombinant proteins; Transgenic plants

Indexed keywords

DEFENSIN;

AMINO ACID SEQUENCE; ARTICLE; GENETICS; IMMUNOLOGY; INNATE IMMUNITY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PERONOSPORA; PHYTOPHTHORA; PLANT DISEASE; POTATO; TOBACCO; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DEFENSINS; IMMUNITY, INNATE; MOLECULAR SEQUENCE DATA; PERONOSPORA; PHYTOPHTHORA; PLANT DISEASES; PLANTS, GENETICALLY MODIFIED; SOLANUM TUBEROSUM; TOBACCO;

EID: 77957890949     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2010.00501.x     Document Type: Article

References (45)

1. Ayala, M., Gavilondo, J., Rodríguez, M., Fuentes, A., Enríquez, G., Pérez, L., Cremata, J. and Pujol, M. (2009) Production of Plantibodies in Nicotiana plants. in: Methods in Molecular Biology. Recombinant Proteins from Plants (Faye, L. and Gomord, V., eds), pp. 103-134. New York: Humana Press.
2. Borrás-Hidalgo, O., Thomma, B., Collazo, C., Chacon, O., Borroto, C.J., Ayra, C., Portieles, R., López, Y. and Pujol, M. (2006) EIL2 transcription factor and glutathione synthetase are required for defense of tobacco against tobacco blue mold. Mol. Plant Microbe Interact. 19, 399-406.
3. Borrás-Hidalgo, O., Thomma, B., Silva, Y., Chacon, O. and Pujol, M. (2010) Tobacco blue mould disease caused by Peronospora hyoscyami f. sp. tabacina. Mol. Plant Pathol. 11, 13-18.
4. Broekaert, W.F., Terras, F.R.G., Cammue, B.P.A. and Vanderleyden, J. (1990) An automated quantitative assay for fungal growth inhibition. FEMS Microbiol. Lett. 69, 55-60.
5. Broekaert, W.F., Terras, F.R., Cammue, B.P. and Osborn, R.W. (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108, 1353-1358.
6. Bruce, T.J. and Pickett, J.A. (2007) Plant defence signalling induced by biotic attacks. Curr. Opin. Plant Biol. 10, 387-392.
7. Chacon, O., Hernandez, I., Portieles, R., Lopez, Y., Pujol, M. and Borrás-Hidalgo, O. (2009) Identification of defense-related genes in tobacco responding to black shank disease. Plant Sci. 177, 175-180.
8. Collazo, C., Ramos, P.L., Chacón, O., Borroto, C.J., López, Y., Pujol, M., Thomma, B.P.H.J., Hein, I. and Borrás-Hidalgo, O. (2006) Phenotypical and molecular characterization of the tomato mottle taino virus - Nicotiana megalosiphon interaction. Physiol. Mol. Plant Pathol. 67, 231-236.
9. Csinos, A.S. (1999) Stem and root resistance to tobacco black shank. Plant Dis. 83, 777-780.
10. De-Paula, V.S., Rasear, G., Medeiros, L., Miyamoto, C.A., Almeida, M.S., Kurtenbach, E., Almeida, F.C.L. and Valente, A.P. (2008) Evolutionary relationship between defensins in the Poaceae family strengthened by the characterization of new sugarcane defensins. Plant Mol. Biol., 68, 321-335.
11. Dita, M.A., Brommonschenkel, S.H., Matzuoka, K. and Mizubuti, E. (2006) Components of resistance to early blight in four potato cultivars: effect of leaf position. J. Phytopathol. 153, 1-6.
12. Do, H.M., Lee, S.C., Jung, H.W., Sohn, K.H. and Hwang, B.K. (2004) Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Sci. 166, 1297-1305.
13. Fradin, E.F. and Thomma, B.P.H.J. (2006) Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol. Plant Pathol. 7, 71-86.
14. Francois, I.E., De Bolle, M.F., Dwyer, G., Goderis, I.J., Woutors, P.F. and Verhaert, P.D. (2002) Transgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. Plant Physiol. 128, 1346-1358.
15. Gao, A.G., Hakimi, S.M., Mittanck, C.A., Wu, Y., Woerner, B.M., Stark, D.M., Shah, D.M., Liang, J. and Rommens, C.M. (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat. Biotechnol. 18, 1307-1310.
16. Höfgen, R. and Willmitzer, L. (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 16, 9877.
17. Kirk, W.W., Felcher, K.J., Douches, D.S., Coombs, J.M., Stein, J.M., Baker, K.M. and Hammerschmidt, R. (2001) Effect of host plant resistance and reduced rates and frequencies of fungicide application to control potato late blight. Plant Dis. 85, 1113- 1118.
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 681- 685.
19. Lay, F.T., Schirra, H.J., Scanlon, M.J, Anderson, M.A. and Craik, D.J. (2003) The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to homology model of the crop defensin alfAFP. J. Mol. Bio., 325, 175-188.
20. Lay, F.T. and Anderson, M.A. (2005) Defensins - components of the innate immune system in plants. Curr. Protein Pept. Sci. 6, 85-101.
21. Liu, Y.J., Cheng, C.S., Lai, S.M., Hsu, M.P., Chen, C.S. and Lyu, P.C. (2006) Solution structure of the plant defensin VrD1 from mung bean and its possible role in insecticidal activity against bruchids. Proteins, 63, 777-786.
22. Meyer, B., Houlne, G., Pozueta-Romero, J., Schantz, M.L. and Schantz, R. (1996) Fruit-specific expression of a defensin-type gene family in bell pepper. Plant Physiol. 112, 615-622.
23. Moreno, M., Segura, A. and Garcia-Olmedo, F. (1994) Pseudothionin-St1, a potato peptide active against potato pathogens. Eur. J. Biochem. 223, 135-139.
24. Osborn, R.W., De Samblanx, G.W., Thevissen, K., Goderis, I., Torrekens, S., Van Leuven, F., Attenborough, S., Rees, S.B. and Broekaert, W.F. (1995) Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 368, 257-262.
25. Pace, C.N., Vajdos, F., Fee, L., Grimsley, G. and Gray, T. (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 2411-2423.
26. Parashina, E.V., Serdobinskii, L.A., Kalle, E.G., Lavorova, N.V., Avetisov, V.A., Lunin, V.G. and Naroditskii, B.S. (2000) Genetic engineering of oilseed rape and tomato plants expressing a radish defensin gene. Russ. J. Plant Physiol. 47, 417-423.
27. Park, H.C., Kang, Y.H., Chun, H.J., Koo, J.C., Cheong, Y.H., Kim, C.Y., Kim, M.C., Chung, W.S., Kim, J.C. and Yoo, J.H. (2002) Characterization of a stamen specific cDNA encoding a novel plant defensin in Chinese cabbage. Plant Mol. Biol. 50, 59-69.
28. Penninckx, I.A., Eggermont, K., Terras, F.R., Thomma, B.P., De Samblanx, G.W., Buchala, A., Metraux, J.P., Manners, J.M. and Broekaert, W.F. (1996) Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acidindependent pathway. Plant Cell, 8, 2309-2323.
29. Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
30. Sels, J., Mathys, J., De Coninck, B.M.A., Cammue, B.P.A. and De Bolle, M.F.C. (2008) Plant pathogenesis-related (PR) proteins: a focus on PR peptides. Plant Physiol. Biochem. 46, 941-950.
31. Solis, J., Medrano, G. and Ghislain, M. (2007) Inhibitory effect of a defensin gene from the Andean crop maca (Lepidium meyenii) against Phytophthora infestans. J. Plant Physiol. 164, 1071-1082.
32. Soto, N., Enríquez, G.A., Ferreira, A., Corrada, M., Fuentes, A., Tiel, K. and Pujol, M. (2007) Efficient transformation of potato stems segments from cultivar Désirée, using phosphinothricin as selection marker. Biotecnol. Apl. 24, 139-144.
33. Spelbrink, R.G., Dilmac, N., Allen, A., Smith, T.J., Shah, D.M. and Hockerman, G.H. (2004) Differential antifungal and calcium channel-blocking activity among structurally related plant defensins. Plant Physiol. 135, 2055-2067.
34. Terras, F.R., Schoofs, H.M., De Bolle, M.F., Van Leuven, F., Rees, S.B., Vanderleyden, J., Cammue, B.P. and Broekaert, W.F. (1992) Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267, 15301-15309.
35. Terras, F.R., Eggermont, K., Kovaleva, V., Raikhel, N.V., Osborn, R.W., Kester, A., Rees, S.B., Vanderleyden, J., Cammue, B.P. and Broekaert, W.F. (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell, 7, 573-588.
36. Thevissen, K., Cammue, B.P.A., Lemaire, K., Winderickx, J., Dickson, R.C., Lester, R.L., Ferket, K.K., Van Even, F., Parret, A.H. and Broekaert, W.F. (2000) A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii). Proc. Natl Acad. Sci. USA, 97, 9531-9536.
37. Thevissen, K., Warnecke, D.C., Francois, I.E.J.A., Leipelt, M., Heinz, E., Ott, C., Zahringer, U., Thomma, B.P.H.J., Ferket, K.K. and Cammue, B.P.A. (2004) Defensins from insects and plants interact with fungal glucosylceramides. J. Biol. Chem. 279, 3900-3905.
38. Thevissen, K., Kristensen, H.H., Thomma, B.P.H.J., Cammue, B.P.A. and François, I.E.J.A. (2007) Therapeutic potential of antifungal plant and insect defensins. Drug Discov. Today, 12, 966-971.
39. Thomma, B.P.H.J. and Broekaert, W.F. (1998) Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in Arabidopsis thaliana. Plant Physiol. Biochem. 36, 533-537.
40. Thomma, B.P.H.J., Cammue, B.P.A. and Thevissen, K. (2002) Plant defensins. Planta, 216, 193-202.
41. Thomma, B.P.H.J., Cammue, B.P.A. and Thevissen, K. (2003) Mode of action of plant defensins suggests therapeutic potential. Curr. Drug Targets Infect. Disord. 3, 1-8.
42. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882.
43. Vleeshouwers, V.G.A.A., van Dooijeweert, W., Keizer, L.C.P., Sijpkes, L., Govers, F. and Colon, L.T. (1999) A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation. Eur. J. Plant Pathol. 105, 241-250.
44. Wang, Y.P., Nowak, G., Culley, D., Hadwiger, L.A. and Fristensky, B. (1999) Constitutive expression of pea defense gene DRR206 confers resistance to blackleg (Leptosphaeria maculans) disease in transgenic canola (Brassica napus). Mol. Plant Microbe Interact. 12, 410-418.
45. Zhu, Y.J., Agbayani, R. and Moore, P.H. (2007) Ectopic expression of Dahlia merckii defensin DmAMP1 improves papaya resistance to Phytophthora palmivora by reducing pathogen vigor. Planta, 226, 87-97.