A possible role of the Non-GATl GABA transporters in transfer of GABA from GAB aergic to glutamatergic neurons in mouse cerebellar neuronal cultures

Neurochemical Research

Volumn 35, Issue 9, 2010, Pages 1384-1390

  Sunol C ^a   Babot, Z ^a   Cristofol R ^a   Sonnewald, U ^b   Waagepetersen, H S ^c   Schousboe, A ^c  

^a INST D INVESTIGACIONS B   (Spain)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

BGT1; Cerebellar granule neurons; GABA; GAT2; GAT3; Transport

Indexed keywords

1 (4,4 DIPHENYL 3 BUTENYL)NIPECOTIC ACID; 4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID CARRIER; 4 AMINOBUTYRIC ACID RECEPTOR; BETA ALANINE; BETAINE; GLUTAMATE DECARBOXYLASE; GUVACINE; NIPECOTIC ACID; TIAGABINE; VESICULAR GLUTAMATE TRANSPORTER 1;

4 AMINOBUTYRIC ACID UPTAKE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELLULAR DISTRIBUTION; CEREBELLUM; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; GOLGI COMPLEX; GRANULE CELL; IC 50; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MOLECULAR MECHANICS; MOUSE; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; STELLATE CELL;

ANIMALS; BETAINE; CELLS, CULTURED; CEREBELLUM; GABA AGENTS; GABA AGONISTS; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; GAMMA-AMINOBUTYRIC ACID; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; LIPOTROPIC AGENTS; MICE; NEURONS; NIPECOTIC ACIDS; VESICULAR GLUTAMATE TRANSPORT PROTEIN 1;

MUS;

EID: 77957853883     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-010-0196-1     Document Type: Article

References (29)

1. Sonnewald U, Olstad E, Qu H, Babot Z, Cristòfol R, Suñol C, Schousboe A, Waagepetersen HS (2004) First direct demonstration of extensive GABA synthesis in mouse cerebellar neuronal cultures. J Neurochem 91:796-803 (Pubitemid 39507356)
2. Sonnewald U, Kortner TM, Qu H, Olstad E, Suñol C, Bak LK, Schousboe A, Waagepetersen HS (2006) Demonstration of extensive GABA synthesis in A small population of GAD positive neurons in cerebellar cultures by the use of pharmacological tools. Neurochem Int 48:572-578
3. Pearce BR, Currie DN, Beale R, Dutton GR (1981) Potassium-stimulated, calcium-dependent release of [3H]GABA from neuron-and glia-enriched cultures of cells dissociated from rat cerebellum. Brain Res 206:485-489 (Pubitemid 11170232)
4. Drejer J, Schousboe A (1989) Selection of A pure cerebellar granule cell culture by kainate treatment. Neurochem Res 14:751-754 (Pubitemid 19255830)
5. Madsen K, White HS, Clausen RP, Frølund B, Larsson OM, Krogsgaard-Larsen P, Schousboe A (2007) Functional and pharmacological aspects of GABA transporters. In: Lajtha A, Reith M (eds) Handbook of neurochemistry and molecular neu-robiology: neural membranes and transport, 3rd edn. Springer, Berlin, pp 285-304
6. Madsen KB, Larsson OM, Schousboe A (2008) Regulation of excitation by GABA neurotransmission: focus on metabolism and transport. Results Probl Cell Differ 44:201-221
7. Clausen RP, Frølund B, Larsson OM, Schousboe A, Krogsgaard-Larsen P, White HS (2006) A novel selective y-aminobutyric acid transport inhibitor demonstrates A functional role for GABA transporter subtype GAT2/BGT-1 in the CNS. Neurochem Int 48:637-642
8. Schousboe A, Thorbek P, Hertz L, Krogsgaard-Larsen P (1979) Effects of GABA analogues of restricted conformation on GABA transport in astrocytes and brain cortex slices and on GABA receptor binding. J Neurochem 33:181-189 (Pubitemid 9233924)
9. Liu Q-R, López-Corcuera B, Mandiyan S, Nelson H, Nelson N (1993) Molecular characterization of four pharmacologically distinct γ-aminobutyric acid transporters in mouse brain. J Biol Chem 268:2106-2112 (Pubitemid 23033620)
10. Borden LA (1996) GABA transporter heterogeneity: pharmacology and cellular localization. Neurochem Int 29:335-356 (Pubitemid 26297678)
11. Saito K, Barber R, Wu J, Matsuda T, Roberts E, Vaughn JE (1974) Immunohistochemical localization of glutamate decar-boxylase in rat cerebellum. Proc Natl Acad Sci USA 71:269-273
12. Erickson JD, de Gois S, Varoqui H, Shafer MK, Weihe E (2006) Activity-dependent regulation of vesicular glutamate and GABA transporters: A means to scale quantal size. Neurochem Int 48:643-649
13. Schousboe A, Meier E, Drejer J, Hertz L (1989) Preparation of primary cultures of mouse (Rat) cerebellar granule cells. In: Shahar A, de Vellis J, Vernadakis A, Haber B (eds) A dissection and tissue culture manual of the nervous system. Alan R. Liss Inc, New York, pp 203-206
14. Bak LK, Schousboe A, Sonnewald U, Waagepetersen HS (2006) Glucose is necessary to maintain neurotransmitter homeostasis during synaptic activity in cultured glutamatergic neurons. J Cereb Blood Flow Metab 26:1285-1297 (Pubitemid 44435243)
15. Hertz L, Juurlink BHJ, Szuchet S (1985) Cell cultures. In: Lajtha A (ed) Handbook of Neurochemistry, vol 8. Plenum Publ Corp, New York, pp 603-661
16. Larsson OM, Johnston GAR, Schousboe A (1983) Differences in uptake kinetics of cis-3-aminocyclohexane carboxylic acid into neurons and astrocytes in primary cultures. Brain Res 260:279-285 (Pubitemid 13181994)
17. Lopez-Corcuera B, Liu QR, Mandiyan S, Nelson H, Nelson N (1992) Expression of A mouse brain cDNA encoding novel gamma-aminobutyric acid transporter. J Biol Chem 267:17491-173493
18. White WF, Snodgrass SR, Dichter M (1980) Identification of GABA neurons in rat cortical cultures by GABA uptake autoradiography. Brain Res 190:139-152 (Pubitemid 10085557)
19. Suñol C, Babot Z, Fonfría E, Galofré M, García D, Herrera N, Iraola S, Vendrell I (2008) Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity. Toxicol In Vitro 22:1350-1355
20. Durkin MM, Smith KE, Borden LA, Weinshank RL, Branchek TA, Gustafson EL (1995) Localization of messenger RNAs encoding three GABA transporters in rat brain: an in situ hybridization study. Mol Brain Res 33:7-21
21. Somogyi P, Hodgson AJ, Chubb IW, Penke B, Erdei A (1985) Antisera to gamma-aminobutyric acid. II. Immunocytochemical application to the central nervous system. J Histochem Cytochem 33:240-248 (Pubitemid 15130012)
22. Storm-Mathiesen J, Ottersen OP (1987) Tracing of neurons with glutamate or gamma-aminobutyrate as putative transmitters. Biochem Soc Trans 15:210-213
23. Ottersen OP (1987) Postembedding light-and electron microscopic immunocytochemistry of amino acids: description of A new model system allowing identical conditions for specificity testing and tissue processing. Exp Brain Res 69:167-174
24. Schousboe A, Larsson OM, Sarup A, White HS (2004) Role of the betaine/GABA transporter (BGT-1/GAT2) for the control of epilepsy. Eur J Pharmacol 500:281-287 (Pubitemid 39304015)
25. White HS, Watson WP, Hansen S, Slough S, Sarup A, Bolvig T, Petersen G, Larsson OM, Clausen RP, Frølund B, Krogsgaard-Larsen P, Schousboe A (2005) First demonstration of A functional role for CNS betaine/GABA transporter (mGAT2) based on synergistic anticonvulsant action among inhibitors of mGAT1 and mGAT2. J Pharmacol Exp Ther 312:866-874
26. Smith MD, Saunders GW, Wilcox KS, Clausen RP, Frølund B, Krogsgaard-Larsen P, Larsson OM, Schousboe A, White HS (2008) Inhibition of the betaine-GABA transporter (mGAT2/ BGT-1) modulates spontaneous electrographic bursting in the medial entorhinal cortex (mEC). Epilepsy Res 79:6-13
27. Madsen KB, Clausen RP, Larsson OM, Krogsgaard-Larsen P, Schousboe A, White HS (2009) Synaptic and extrasynaptic GABA transporters as targets for anti-epileptic drugs. J Neuro-chem 109(suppl 1):139-144
28. Madsen KK, White HS, Schousboe A (2010) Neuronal and nonneuronal GABA transporters as targets for antiepileptic drugs. Pharmacol Ther 125:394-401
29. Rossi DJ, Hamann M, Attwell D (2003) Multiple modes of GABAergic inhibition of rat cerebellar granule cells. J Physiol 548:97-110 (Pubitemid 36410800)