Stabilization of human urine doping control samples: IV. Human chorionic gonadotropin

Analytical and Bioanalytical Chemistry

Volumn 398, Issue 3, 2010, Pages 1313-1318

  Tsivou, Maria ^a,c   Dimopoulou, Helen A ^a   Georgakopoulos, Dimitris G ^b   Koupparis, Michael A ^c   Atta Politou, Julia ^c   Georgakopoulos, Costas G ^a  

^a OAKA   (Greece)

^b AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^c UNIVERSITY OF ATHENS   (Greece)

Author keywords

Doping control analysis; Human chorionic gonadotropin; Microorganisms; Proteases; Stabilization; Urine

Indexed keywords

CHEMICAL MIXTURES; CHEMICAL STABILIZATION; DOPING CONTROL; DOPING CONTROL ANALYSIS; E. COLI; ELEVATED TEMPERATURE; ENDOGENOUS STEROIDS; HIGH TEMPERATURE; HUMAN CHORIONIC GONADOTROPINS; HUMAN URINE; PROTEASE INHIBITOR; PROTEASES; PROTEOLYTIC ENZYME; RECOMBINANT ERYTHROPOIETIN (REPO); URINARY TRACT INFECTIONS; URINE; URINE SAMPLE; WORLD ANTI-DOPING AGENCY;

BODY FLUIDS; CATALYSTS; CHEMICAL ANALYSIS; DEGRADATION; DOPING (ADDITIVES); ENZYMES; ESCHERICHIA COLI; EXPERIMENTS; MICROORGANISMS; MIXTURES;

STABILIZATION;

CHORIONIC GONADOTROPIN;

CONFERENCE PAPER; DOPING; ENZYME IMMUNOASSAY; HUMAN; STANDARD; URINE;

CHORIONIC GONADOTROPIN; DOPING IN SPORTS; HUMANS; IMMUNOENZYME TECHNIQUES; REFERENCE STANDARDS;

ESCHERICHIA COLI;

EID: 77957841882     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-4033-9     Document Type: Conference Paper

References (21)

1. U-H Stenman K Hotakainen H Alfthan 2008 Gonadotropins in doping: pharmacological basis and detection of illicit use Br J Pharmacol 154 569 583 1:CAS:528:DC%2BD1cXmt1KqtL8%3D 10.1038/bjp.2008.102
2. JF O'Connor S Birken JW Lustbader A Krichevsky Y Chen RE Canfield 1994 Recent advances in the chemistry and immunochemistry of human chorionic gonadotropin: impact on clinical measurements Endocr Rev 15 650 683
3. M Kohler A Thomas H Geyer L Horta W Schänzer M Thevis 2009 Detection of the protease Bacillolysin in doping-control urine samples Drug Test Analysis 1 143 145 1:CAS:528:DC%2BD1MXhtVyntr%2FN 10.1002/dta.29
4. A Thomas M Kohler K Walpurgis W Schänzer M Thevis 2009 Proteolysis and autolysis of proteases and the detection of degradation products in doping control Drug Test Analysis 1 81 86 1:CAS:528:DC%2BD1MXkvFGmtbc%3D 10.1002/dta.20
5. M Thevis J Maurer M Kohler W Schänzer 2007 Proteases in doping control analysis Int J Sports Med 28 545 549 1:CAS:528:DC%2BD2sXpslOrt7Y%3D 10.1055/s-2007-965159
6. Anielski P, Schwenke D, Monecke S, Jacobs E, Thieme D (2009) Bacterial proteases in urine samples - effects on EPO analysis? In: Schänzer W, Geyer H, Gotzmann A, Mareck U (eds) Recent advances in doping analysis (17). Sportverlag Strau, Köln, pp 281-284
7. MR Maurizi 1992 Proteases and protein degradation in Escherichia coli Experientia 48 178 201 1:CAS:528:DyaK38XhvFSltLc%3D 10.1007/BF01923511
8. M Tsivou D Livadara DG Georgakopoulos M Koupparis J Atta-Politou CG Georgakopoulos 2009 Stabilization of human urine doping control samples: II. Microbial degradation of steroids Anal Biochem 388 146 154 1:CAS:528: DC%2BD1MXktFamsbg%3D 10.1016/j.ab.2009.02.013
9. M Tsivou HA Dimopoulou DG Georgakopoulos M Koupparis J Atta-Politou CG Georgakopoulos 2010 Stabilization of human urine doping control samples: III. Recombinant human erythropoietin Clin Chim Acta 411 448 452 1:CAS:528: DC%2BC3cXhtlCmsbk%3D 10.1016/j.cca.2009.11.029
10. UH Stenman L Unkila-Kallio J Korhonen H Alfthan 1997 Immunoprocedures for detecting human chorionic gonadotrophin: clinical aspects and doping control Clin Chem 43 1293 1298 1:CAS:528:DyaK2sXktlCkur4%3D
11. LA Cole 1997 Immunoassay of human chorionic gonadotrophin, its free subunits, and metabolites Clin Chem 43 2233 2243 1:CAS:528:DyaK1cXis1GitA%3D%3D
12. S Birken P Berger JM Bidart M Weber A Bristow R Normanm C Sturgeon UH Stenman 2003 Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites Clin Chem 49 144 154 1:CAS:528:DC%2BD3sXktFGmug%3D%3D 10.1373/49.1.144
13. UH Stenman 2004 Standardization of assays for human chorionic gonadotropin Clin Chem 50 798 800 1:CAS:528:DC%2BD2cXjvVaktrk%3D 10.1373/clinchem.2003.031013
14. LA Cole JM Sutton TN Higgins GS Cembrowski 2004 Between-method variation in human chorionic gonadotropin test results Clin Chem 50 5 874 882 1:CAS:528:DC%2BD2cXjvVakt7k%3D 10.1373/clinchem.2003.026989
15. LA Cole 2009 Human chorionic gonadotropin tests Expert Rev Mol Diagn 9 721 747 1:CAS:528:DC%2BD1MXht1CmurrK 10.1586/erm.09.51
16. A Bristow P Berger JM Bidart S Birken R Norman UH Stenman C Sturgeon 2005 Establishment, value assignment, and characterization of new WHO reference reagents for six molecular forms of human chorionic gonadotropin Clin Chem 51 177 182 1:CAS:528:DC%2BD2MXlt1ymtw%3D%3D 10.1373/clinchem.2004.038679
17. LA Cole 2009 New discoveries on the biology and detection of human chorionic gonadotropin Reprod Biol Endocrinol 7 8 45 10.1186/1477-7827-7-8
18. M Tsivou D Livadara DG Georgakopoulos M Koupparis J Atta-Politou CG Georgakopoulos 2009 Stabilization of human urine doping control samples Anal Biochem 388 179 191 1:CAS:528:DC%2BD1MXkvVWgtbk%3D 10.1016/j.ab.2009.02.016
19. WADA (2009) Technical document - TD2009MRPL ver. 2.0. Minimum required performance levels for detection of prohibited substances. http://www.wada-ama. org/Documents/World-Anti-Doping-Program/WADP-IS-Laboratories/ WADA-Summary-Modifications-TD2009MRPL-v2.0-EN.pdf. Accessed 16 March 2010
20. U Sancken D Bahner 1995 The effect of thermal instability of intact human chorionic gonadotropin (ihCG) on the application of its free β-subunit (free βhCG) as a serum marker in Down syndrome screening Prenat Diagn 15 731 738 1:CAS:528:DyaK2MXosF2msrg%3D 10.1002/pd.1970150808
21. Tsivou M, Livadara D, Georgakopoulos DG, Koupparis M, Atta-Politou J, Georgakopoulos CG (2008) Preservation of urine doping control samples: preliminary results. In: Schänzer W, Geyer H, Gotzmann A, Mareck U (eds) Recent advances in doping analysis (16). Sportverlag Strau, Köln, pp 41-50