1,3-β-d-Glucan synthase of Paracoccidioides brasiliensis: Recombinant protein, expression and cytolocalization in the yeast and mycelium phases

Fungal Biology

Volumn 114, Issue 10, 2010, Pages 809-816

  Tomazett, Patrícia Kott ^a   Félix, Carlos Roberto ^b   Lenzi, Henrique Leonel ^c   de Paula Faria, Fabrícia ^a   de Almeida Soares, Célia Maria ^a   Pereira, Maristela ^a  

^a FEDERAL UNIVERSITY OF GOIÁS   (Brazil)

^b UNIVERSITY OF BRASILIA   (Brazil)

^c INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

1,3 d glucan synthase; Cell wall; Cytolocalization; Paracoccidioides brasiliensis; Recombinant protein

Indexed keywords

FUNGAL PROTEIN; GLUCAN; GLUCAN SYNTHASE; GLUCOSYLTRANSFERASE; RECOMBINANT PROTEIN;

BACTERIUM; CYTOLOGY; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; IMMUNOASSAY; PATHOGENICITY; POLYMER; PROTEIN; RECOMBINATION; YEAST;

ARTICLE; CELL WALL; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; MICROBIOLOGY; MYCELIUM; PARACOCCIDIOIDES; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; SOUTH AMERICAN BLASTOMYCOSIS;

CELL WALL; FUNGAL PROTEINS; GENE EXPRESSION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; GLUCANS; GLUCOSYLTRANSFERASES; HUMANS; MYCELIUM; PARACOCCIDIOIDES; PARACOCCIDIOIDOMYCOSIS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; FUNGI; PARACOCCIDIOIDES BRASILIENSIS;

EID: 77957662524     PISSN: 18786146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.funbio.2010.07.007     Document Type: Article

References (39)

1. Almeida A.J., Carmona J.A., Cunha C., Carvalho A., Rappleye C.A., Goldman W.E., Hooykaas P.J., Leao C., Ludovico P., Rodrigues F. Towards a molecular genetic system for the pathogenic fungus Paracoccidioides brasiliensis. Fungal Genetics Biology 2007, 44:1387-1398.
2. Arellano M., Duran A., Perez P. Rho 1 GTPase activates the (1-3)beta-d-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis. Embo Journal 1996, 15:4584-4591.
3. Beauvais A., Bruneau J.M., Mol P.C., Buitrago M.J., Legrand R., Latge J.P. Glucan synthase complex of Aspergillus fumigatus. Journal of Bacteriology 2001, 183:2273-2279.
4. Beauvais A., Maubon D., Park S., Morelle W., Tanguy M., Huerre M., Perlin D.S., Latge J.P. Two alpha(1-3) glucan synthases with different functions in Aspergillus fumigatus. Applied and Environmental Microbiology 2005, 71:1531-1538.
5. Billon-Grand G., Marais M.F., Joseleau J.P., Girard V., Gay L., Fevre M. A novel 1,3-β-glucan synthase from the oomycete Saprolegnia monoica. Microbiology 1997, 143:3175-3183.
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976, 72:248-254.
7. Brown G.D. Dectin-1: a signalling non-TLR pattern-recognition receptor. Nature Reviews Immunology 2006, 6:33-43.
8. Carbonell L.M., Kanetsuna F., Gil F. Chemical morphology of glucan and chitin in the cell wall of the yeast phase of Paracoccidioides brasiliensis. Journal of Bacteriology 1970, 101:636-642.
9. Castro N.S., Barbosa M.S., Maia Z.A., Bao S.N., Felipe M.S., Santana J.M., Soares Mendes-Giannini M.J., Pereira M., Soares C.M.A. Characterization of Paracoccidioides brasiliensis PbDfg5p, a cell-wall protein implicated in filamentous growth. Yeast 2008, 25:141-154.
10. Castro N.S., Castro K.P., Orlandi I., Feitosa L.S., Rosa e Silva L.K., Vainstein M.H., Bao S.N., Vai M., Soares C.M. Characterization and functional analysis of the beta-1,3-glucanosyltransferase 3 of the human pathogenic fungus Paracoccidioides brasiliensis. FEMS Yeast Research 2009, 9:103-114.
11. Delom F., Szponarski W., Sommerer N., Boyer J.C., Bruneau J.M., Rossignol M., Gibrat R. The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor. Proteomics 2006, 6:3029-3039.
12. Drgonová J., Drgon T., Tanaka K., Kollár R., Chen G.C., Ford R.A., Chan C.S., Takai Y., Cabib E. Rho1p, a yeast protein at the interface between cell polarization and morphogenesis. Science 1996, 272:277-279.
13. Guest G.M., Lin X., Momany M. Aspergillus nidulans RhoA is involved in polar growth, branching, and cell wall synthesis. Fungal Genetics Biology 2004, 41:13-22.
14. Inoue S.B., Qadota H., Arisawa M., Anraku Y., Watanabe T., Ohya Y. Signaling toward yeast 1,3-beta-glucan synthesis. Cell Structure and Function 1996, 21:395-402.
15. Inoue K., Takano H., Koike E., Yanagisawa R., Oda T., Tamura H., Adachi Y., Ishibashi K., Ohno N. Candida soluble cell wall beta-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: possible role of antigen-presenting cells. Respiratory Research 2009, 10:68.
16. Kanetsuna F., Carbonell L.M. Cell wall glucans of the yeast and mycelial forms of Paracoccidioides brasiliensis. Journal of Bacteriology 1970, 101:675-680.
17. Kanetsuna F., Carbonell L.M., Azuma I., Yamamura Y. Biochemical studies on the thermal dimorphism of Paracoccidioides brasiliensis. Journal of Bacteriology 1972, 110:208-218.
18. Kellner E.M., Orsborn K.I., Siegel E.M., Mandel M.A., Orbach M.J., Galgiani J.N. Coccidioides posadasii contains a single 1,3-beta-glucan synthase gene that appears to be essential for growth. Eukaryotic Cell 2005, 4:111-120.
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
20. Latge J.P. The cell wall: a carbohydrate armour for the fungal cell. Molecular Microbiology 2007, 66:279-290.
21. Lesage G., Bussey H. Cell wall assembly in Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews 2006, 70:317-343.
22. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
23. Mazur P., Baginsky W. In vitro activity of 1,3-beta-d-glucan synthase requires the GTP-binding protein Rho1. Journal of Biological Chemistry 1996, 271:14604-14609.
24. Mazur P., Morin N., Baginsky W., el-Sherbeini M., Clemas J.A., Nielsen J.B., Foor F. Differential expression and function of two homologous subunits of yeast 1,3-beta-d-glucan synthase. Molecular and Cellular Biology 1995, 15:5671-5681.
25. Mio T., Adachi-Shimizu M., Tachibana Y., Tabuchi H., Inoue S.B., Yabe T., Yamada-Okabe T., Arisawa M., Watanabe T., Yamada-Okabe H. Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in beta-1,3-glucan synthesis. Journal of Bacteriology 1997, 179:4096-4105.
26. Pereira M., Felipe M.S., Brigido M.M., Soares C.M., Azevedo M.O. Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungus Paracoccidioides brasiliensis. Yeast 2000, 16:451-462.
27. Pitarch A., Sanchez M., Nombela C., Gil C. Sequential fractionation and two-dimensional gel analysis unravels the complexity of the dimorphic fungus Candida albicans cell wall proteome. Molecular and Cellular Proteomics 2002, 1:967-982.
28. Qadota H., Python C.P., Inoue S.B., Arisawa M., Anraku Y., Zheng Y., Watanabe T., Levin D.E., Ohya Y. Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 1996, 272:279-281.
29. Roh D.H., Bowers B., Riezman H., Cabib E. Rho1p mutations specific for regulation of beta(1,3)glucan synthesis and the order of assembly of the yeast cell wall. Molecular Microbiology 2002, 44:1167-1183.
30. Ruiz-Herrera J., Xoconostle-Cazares B., Reynaga-Pena C.G., Leon-Ramirez C., Carabez-Trejo A. Immunolocalization of chitin synthases in the phytopathogenic dimorphic fungus Ustilago maydis. FEMS Yeast Research 2006, 6:999-1009.
31. Sambrook J., Russel D.W. Molecular Cloning. A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York.
32. San-Blas G., San-Blas F. Effect of detergents on membrane-associated glucan synthetase from Paracoccidioides brasiliensis. Journal of Bacteriology 1982, 152:563-566.
33. Silva S.P., Felipe M.S.S., Pereira M., Azevedo M.O., Soares C.M.A. Phase transition and stage-specific protein synthesis in the dimorphic fungus Paracoccidioides brasiliensis. Experimental Mycology 1994, 18:294-299.
34. da Silva Neto B.R., de Fatima da Silva J., Mendes-Giannini M.J., Lenzi H.L., de Almeida Soares C.M., Pereira M. The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin. BMC Microbiology 2009, 9:272.
35. Soares R.B., Velho T.A., De Moraes L.M., Azevedo M.O., Soares C.M., Felipe M.S. Hygromycin B-resistance phenotype acquired in Paracoccidioides brasiliensis via plasmid DNA integration. Medical Mycology 2005, 43:719-723.
36. Sorais F., Barreto L., Leal J.A., Bernabe M., San-Blas G., Nino-Vega G.A. Cell wall glucan synthases and GTPases in Paracoccidioides brasiliensis. Medical Mycology 2010, 46:35-47.
37. Thompson J.R., Douglas C.M., Li W., Jue C.K., Pramanik B., Yuan X., Rude T.H., Toffaletti D.L., Perfect J.R., Kurtz M. A glucan synthase FKS1 homolog in Cryptococcus neoformans is single copy and encodes an essential function. Journal of Bacteriology 1999, 181:444-453.
38. Utsugi T., Minemura M., Hirata A., Abe M., Watanabe D., Ohya Y. Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes to Cells 2002, 7:1-9.
39. Zambuzzi-Carvalho P.F., Cruz A.H., Santos-Silva L.K., Goes A.M., Soares C.M., Pereira M. The malate synthase of Paracoccidioides brasiliensis Pb01 is required in the glyoxylate cycle and in the allantoin degradation pathway. Medical Mycology 2009, 47:734-744.