메뉴 건너뛰기




Volumn 20, Issue 19, 2010, Pages 5827-5830

Synthesis and biological evaluation of novel coumarin-based inhibitors of Cdc25 phosphatases

Author keywords

Cdc25 phosphatases; Chalcones; Coumarins; Heck coupling

Indexed keywords

BN 82002; COUMARIN DERIVATIVE; PROTEIN TYROSINE PHOSPHATASE; PROTEIN TYROSINE PHOSPHATASE INHIBITOR; UNCLASSIFIED DRUG;

EID: 77957590019     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.07.130     Document Type: Article
Times cited : (37)

References (32)
  • 29
    • 77957560053 scopus 로고    scopus 로고
    • note
    • The enzymatic activity of the GST-Cdc25 recombinant enzyme was performed in 96-well plates in [50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA and 0.1% SAB, pH 8,1] buffer containing 3-O-methylfluorescein phosphate 500 μM as substrate. The Gst-Cdc25 proteins, diluted in assay buffer, were used at a final concentration of 1 μg/well. After 2 h at 30 °C, 3-O-methylfluorescein fluorescent emission was measured with a CytoFluor system Perspective Applied Biosystems; excitation filter: 475 nm and emission filter: 510 nm.
  • 30
    • 77957594813 scopus 로고    scopus 로고
    • All compounds were characterized by HRMS (ESI) and NMR analysis
    • (a) All compounds were characterized by HRMS (ESI) and NMR analysis.
  • 31
    • 77957552952 scopus 로고    scopus 로고
    • note
    • +.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.