The role of electrically stimulated endocytosis in gene electrotransfer

IFMBE Proceedings

Volumn 29, Issue , 2010, Pages 679-682

  Pavlin, M ^a   Kanduser M ^a   Pucihar, G ^a   Miklavcic D ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

cells; electroporation; endocytosis; gene electrotransfer

Indexed keywords

DNA TRANSFER; ELECTRIC FIELD STRENGTH; ELECTRIC PULSE; ELECTROPORATION; ELECTROTRANSFER; ENDOCYTOSIS; GENE DELIVERY; HIGH VOLTAGE PULSE; PULSE PARAMETER; TEMPERATURE DEPENDENCIES; THRESHOLD ELECTRIC FIELDS; THRESHOLD FIELDS; TRANSFECTION EFFICIENCY;

BIOCHEMICAL ENGINEERING; CELL MEMBRANES; CYTOLOGY; DNA; ELECTRIC FIELDS; ELECTRIC POTENTIAL; GENE THERAPY; GENE TRANSFER; MEDICAL COMPUTING; MOLECULAR BIOLOGY;

GENE EXPRESSION;

EID: 77957578377     PISSN: 16800737     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1007/978-3-642-13039-7_171     Document Type: Conference Paper

References (13)

1. Wong TK, Neumann E (1982) Electric-field mediated gene-transfer. Biochem Biophys Res Com 107: 584-587
2. Neumann E, Schaeferridder M, Wang Y et al. (1982) Gene-transfer into mouse lyoma cells by electroporation in high electric-fields. EMBO J 1: 841-845
3. Escoffre JM, Mauroy C, Portet T et al. (2009) Gene electrotransfer: from biophysical mechanisms to in vivo applications, Biophysical Reviews, 1: 177-184
4. Prud'homme GJ, Glinka Y, Khan AS et al. (2006) Electroporation-enhanced nonviral gene transfer for the prevention or treatment of immunological, endocrine and neoplastic diseases. Curr Gene Ther. 6: 243-273
5. Daud AI, DeConti RC, Andrews S et al. (2008) Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma. J Clin Oncol 26: 5896-5903
6. Ferber D (2001) Gene therapy: safer and virus free? Science 294: 1638-1642
7. Golzio M, Teissié J, Rols MP (2002) Direct visualization at the single cell level of electrically mediated gene delivery. Proc Natl Acad Sci USA 99: 1292-1297
8. Čemazar M, Golzio M, Serša G, (2006) Electrically-Assisted Nucleic Acids Delivery to Tissues In Vivo: Where Do We Stand? Curr Pharm Des, 12: 3817-3825
9. Šatkauskas S, Bureau MF, Mahfoudi A et al. (2001) Mol Ther 4: 317-323
10. Glogauer M, Lee W, McCulloch AG (1993) Induced endocytosis in human fibroblasts by electrical fields. Exp Cell Res. 208: 232-240
11. Rols MP, Femenina P, Teissié J (1995) Long-lived macropinocytosis takes place in electropermeabilized mammalian cells. Biochem Biophys Res Com 208: 26-35
12. Zimmermann U, Schnettler R, Klock et al. (1990) Mechanisms of electrostimulated uptake of macromolecules into living cells. Natur-wissenschaften 77: 543-545
13. Kandušer M, Miklavčič D, Pavlin M (2009) Mechanisms involved in gene electrotransfer using high- and low-voltage pulses - an in vitro study. Bioelectrochem 74: 265-271