A high-throughput method for the quantitative analysis of auxins

Nature Protocols

Volumn 5, Issue 10, 2010, Pages 1609-1618

  Barkawi, Lana S ^a   Tam, Yuen Yee ^b,c   Tillman, Julie A ^a   Normanly, Jennifer ^b   Cohen, Jerry D ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c Aurora Biofuels Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUXIN; DIAZOMETHANE; INDOLEACETIC ACID; POLY(METHYL METHACRYLATE);

ACCURACY; ARTICLE; AUTOMATION; CONTROLLED STUDY; DATA ANALYSIS; DERIVATIZATION; HARVESTING; HIGH THROUGHPUT SCREENING; HORMONE DETERMINATION; ISOTOPE DILUTION ASSAY; MASS FRAGMENTOGRAPHY; NONHUMAN; PLANT TISSUE; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; ROBOTICS; SOLID PHASE EXTRACTION;

AUTOMATION; CARBON ISOTOPES; DIAZOMETHANE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; INDOLEACETIC ACIDS; METHYLATION; PLANT EXTRACTS; PLANT GROWTH REGULATORS; PLANTS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOLID PHASE EXTRACTION;

EID: 77957569130     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.118     Document Type: Article

References (46)

1. Cielieski, T. Untersuchungen über die Abwärtskrümmung der Wurzel. Beitr. Biol. Pfanz. 1, 1-30 (1874).
2. Darwin, C.R. The Power of Movement in Plants (John Murray, 1880).
3. Woodward, A.W. & Bartel, B. Auxin: regulation, action, and interaction. Ann. Bot. 95, 707-735 (2005).
4. Went, F.W. & Thimann, K.V. Phytohormones (The Macmillan Company, 1937).
5. Jensen, P.B. Growth Hormones in Plants (McGraw-Hill, 1936).
6. Thimann, K.V. In Plant Growth Substances (ed. Skoog, F.) 15-33 (Springer-Verlag, 1979).
7. Remers, W.A. In Indoles (ed. Houlihan, WJ.) 1-226 (Wiley-Interscience, 1972).
8. Ribnicky, D.M., Cooke, T.J. & Cohen, J.D. A microtechnique for the analysis of free and conjugated indole-3-acetic acid in milligram amounts of plant tissue using a benchtop gas chromatograph-mass spectrometer. Planta 204, 1-7 (1998).
9. Turian, G. & Hamilton, R.H. Chemical detection of 3-indolylacetic acid in Ustilago zeae tumors. Biochim. Biophys. Acta 41, 148-150 (1960).
10. Hamilton, R.H., Bandurski, R.S. & Grigsby, B.H. Isolation of indole-3-acetic acid from corn kernels and etiolated corn seedlings. Plant Physiol. 36, 354-359 (1961).
11. Rittenberg, D. & Foster, G.L. A new procedure for quantitative analysis by isotope dilution, with application to the determination of amino acids and fatty acids. J. Biol. Chem. 133, 737-744 (1940).
12. Bandurski, R.S., Schulze, A. & Cohen, J.D. Photo-regulation of the ratio of ester to free indole-3-acetic acid. Biochem. Biophys. Res. Commun. 79, 1219-1223 (1977).
13. Bandurski, R.S. & Schulze, A. Concentrations of indole-3-acetic acid and its esters in Avena and Zea. Plant Physiol. 54, 257-262 (1974).
14. Beale, M.H. In Biochemistry and Molecular Biology of Plant Hormones Vol. 33 New Comprehensive Biochemistry (eds. Hooykaas, P.J.J., Hall, M.A. & Libbenga, K.R.) 62-88 (Elsevier, 1999).
15. Fuchs, S., Haimovich, J. & Fuchs, Y. Immunological studies of plant hormones. Eur. J. Biochem. 18, 384-390 (1971).
16. Weiler, E.W. Immunoassay of plant growth regulators. Annu. Rev. Plant Physiol. 35, 85-95 (1984).
17. Mertens, R., Eberle, J., Arnsheidt, A., Ledebur, A. & Weiler, E.W. Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid. Planta 166, 389-393 (1985).
18. Marcussen, J., Ulvskov, P., Olsen, C.E. & Rajagopal, R. Preparation and properties of antibodies against indoleacetic acid (IAA)-C5-BSA, a novel ring-coupled IAA antigen, as compared to two other types of IAA-specifc antibodies. Plant Physiol. 89, 1071-1078 (1989).
19. Ilic, N. et al. Aminoethyl-substituted indole-3-acetic acids for the preparation of tagged and carrier-linked auxin. Bioorg. Med. Chem. 13, 3229-3240 (2005).
20. Pengelly, W.L., Bandurski, R.S. & Schulze, A. Validation of a radioimmunoassay for indole-3-acetic acid using gas chromatography-selected ion monitoring-mass spectrometry. Plant Physiol. 68, 96-98 (1981).
21. Crozier, A., Sandberg, G., Monteiro, A.M. & Sundberg, B. The use of immunological techniques in plant hormone analysis. In Plant Growth Substances 1985 (ed. Bopp, M.) 13-21 (Springer-Verlag, 1986).
22. 6-labeled internal standard. Plant Physiol. 84, 982-986 (1987).
23. Park, S., Cohen, J.D. & Slovin, J.P. Strawberry fruit protein with a novel indole-acyl modifcation. Planta 224, 1015-1022 (2006).
24. Sundberg, B., Sandberg, G. & Crozier, A. Purifcation of indole-3-acetic acid in plant extracts by immunoaffnity chromatography. Phytochemistry 25, 295-298 (1986).
25. Armstrong, J.I., Yuan, S., Dale, J.M., Tanner, V.N. & Theologis, A. Identifcation of inhibitors of auxin transcriptional activation by means of chemical genetics in Arabidopsis. Proc. Natl. Acad. Sci. USA 101, 14978-14983 (2004).
26. Magnus, V., Bandurski, R.S. & Schulze, A. Synthesis of 4,5,6,7 and 2,4,5,6,7 deuterium-labeled indole-3-acetic acid for use in mass spectrometric assays. Plant Physiol. 66, 775-781 (1980).
27. 6-[benzene ring]-indole-3-acetic acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic acid in plants. Plant Physiol. 80, 14-19 (1986).
28. Chen, K.-H., Miller, A.N., Patterson, G.W. & Cohen, J.D. A rapid and simple procedure for purifcation of indole-3-acetic acid prior to GC-SIM-MS analysis. Plant Physiol. 86, 822-825 (1988).
29. Tam, Y.Y. & Normanly, J. Overexpression of a bacterial indole-3-acetyl-L-aspartic acid hydrolase in Arabidopsis thaliana. Physiol. Plant. 115, 513-522 (2002).
30. Edlund, A., Eklöf, S., Sundberg, B., Moritz, T. & Sandberg, G. A microscale technique for gas chromatography-mass spectrometry measurements of picogram amounts of indole-3-acetic acid in plant tissues. Plant Physiol. 108, 1043-1047 (1995).
31. Kowalczyk, M. & Sandberg, G. Quantitative analysis of indole-3-acetic acid metabolites in Arabidopsis. Plant Physiol. 127, 1845-1853 (2001).
32. Ljung, K. et al. Sites and regulation of auxin biosynthesis in Arabidopsis roots. Plant Cell 17, 1090-1104 (2005).
33. Moritz, T. & Olsen, J.E. Comparison between high-resolution selected ion monitoring, selected reaction monitoring, and four-sector tandem mass spectrometry in quantitative analysis of gibberellins in milligram amounts of plant tissue. Anal. Chem. 67, 1711-1716 (1995).
34. Barkawi, L.S. et al. A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal. Biochem. 372, 177-188 (2008).
35. Barkawi, L.S. & Cohen, J.D. A method for concurrent diazomethane synthesis and substrate methylation in a 96-sample format. Nat Protoc. 5, 1619-1626 (2010).
36. Quint, M., Barkawi, L.S., Fan, K.T., Cohen, J.D. & Gray, W.M. Arabidopsis IAR4 modulates auxin response by regulating auxin homeostasis. Plant Physiol. 150, 748-758 (2009).
37. Sun, J. et al. Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Plant Cell 21, 1495-14511 (2009).
38. Negi, S., Sukumar, P., Liu, X., Cohen, J.D. & Muday, G.K. Genetic dissection of the role of ethylene in regulating auxin-dependent lateral and adventitious root formation in tomato. Plant J. 61, 3-15 (2010).
39. Skirpan, A. et al. BARREN INFLORESCENCE2 interaction with ZmPIN1a suggests a role in auxin transport during maize inforescence development. Plant Cell Physiol. 50, 652-657 (2009).
40. Rao, R.P., Hunter, A., Kashpur, O. & Normanly, J. Aberrant synthesis of indole-3-acetic acid in Saccharomyces cerevisiae triggers morphogenic transition, a virulence trait of pathogenic fungi. Genetics 185, 211-220 (2010).
41. Rivier, L. GC-MS of auxin. In Gas Chromatography/Mass Spectrometry Vol. 3 Modern Method of Plant Analysis (eds. Linskens, H.F. & Jackson, J.F.) 146-188 (Springer-Verlag, 1986).
42. Cohen, J.D. Convenient apparatus for the generation of small amounts of diazomethane. J. Chromatogr. 303, 193-196 (1984).
43. Baraldi, R., Chen, K.-H. & Cohen, J.D. Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (Prunus cerasus I.). J. Chromatogr. A 442, 301-306 (1988).
44. Ljung, K., Bhalerao, R.P. & Sandberg, G. Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth. Plant J. 28, 465-474 (2001).
45. Caruso, J.L. & Zeisler, C.S. Indole-3-acetic acid in Douglas fr seedlings: a reappraisal. Phytochemistry 22, 589-590 (1983).
46. Williams, C.M., Porter, A.H. & Greer, M. Mass Spectrometry of Biologically Important Aromatic Acids Vol. 1 (University of Florida, Gainesville, Florida, USA, 1969)