Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling

Journal of Proteome Research

Volumn 9, Issue 10, 2010, Pages 4952-4959

  Kronewitter, Scott R ^a   De Leoz, Maria Lorna A ^a   Peacock, Kyle S ^a   McBride, Kelly R ^a   An, Hyun Joo ^a   Miyamoto, Suzanne ^b   Leiserowitz, Gary S ^b   Lebrilla, Carlito B ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fourier transform ion cyclotron resonance mass spectrometry; glycomics; human serum; interpretation of mass spectra

Indexed keywords

ALCOHOL; BIOLOGICAL MARKER; GLYCAN;

ANALYTIC METHOD; ARTICLE; BLOOD ANALYSIS; ENZYME RELEASE; GLYCOSYLATION; HUMAN; ION CYCLOTRON RESONANCE MASS SPECTROMETRY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROWAVE RADIATION; PRIORITY JOURNAL; PROTEIN SECRETION; SOLID PHASE EXTRACTION;

BIOLOGICAL MARKERS; BLOOD PROTEINS; GLYCOMICS; HUMANS; MASS SPECTROMETRY; POLYSACCHARIDES; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 77957369944     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100202a     Document Type: Article

References (21)

1. Apweiler, R.; Hermjakob, H.; Sharon, N. On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database Biochim. Biophys. Acta 1999, 1473 (1) 4-8
2. Vance, B. A.; Wu, W. Y.; Ribaudo, R. K.; Segal, D. M.; Kearse, K. P. Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-Cys) glycosylation motifs J. Biol. Chem. 1997, 272 (37) 23117-23122
3. Miletich, J. P.; Broze, G. J. Beta-Protein-C Is Not Glycosylated at Asparagine-329-the Rate of Translation May Influence the Frequency of Usage at Asparagine-X-Cysteine Sites J. Biol. Chem. 1990, 265 (19) 11397-11404
4. Titani, K.; Kumar, S.; Takio, K.; Ericsson, L. H.; Wade, R. D.; Ashida, K.; Walsh, K. A.; Chopek, M. W.; Sadler, J. E.; Fujikawa, K. Amino-Acid-Sequences of Human Vonwillebrand-Factor Biochemistry 1986, 25 (11) 3171-3184
5. Lebrilla, C. B.; An, H. J. The prospects of glycan biomarkers for the diagnosis of diseases Mol. Biosyst. 2009, 5 (1) 17-20
6. Packer, N. H.; von der Lieth, C. W.; Aoki-Kinoshita, K. F.; Lebrilla, C. B.; Paulson, J. C.; Raman, R.; Rudd, P.; Sasisekharan, R.; Taniguchi, N.; York, W. S. Frontiers in glycomics: bioinformatics and biomarkers in disease. An NIH white paper prepared from discussions by the focus groups at a workshop on the NIH campus, Bethesda MD (September 11-13, 2006) Proteomics 2008, 8 (1) 8-20
7. Turnbull, J. E.; Field, R. A. Emerging glycomics technologies Nat. Chem. Biol. 2007, 3 (2) 74-77
8. Barkauskas, D. A.; An, H. J.; Kronewitter, S. R.; de Leoz, M. L.; Chew, H. K.; White, R. W. D.; Leiserowitz, G. S.; Miyamoto, S.; Lebrilla, C. B.; Rocke, D. M. Detecting glycan cancer biomarkers in serum samples using MALDI FT-ICR mass spectrometry data Bioinformatics 2009, 25 (2) 251-257
9. An, H. J.; Miyamoto, S.; Lancaster, K. S.; Kirmiz, C.; Li, B.; Lam, K. S.; Leiserowitz, G. S.; Lebrilla, C. B. Profiling of glycans in serum for the discovery of potential biomarkers for ovarian cancer J. Proteome Res. 2006, 5 (7) 1626-1635
10. de Leoz, M. L. A.; An, H. J.; Kronewitter, S.; Kim, J.; Beecroft, S.; Vinall, R.; Miyamoto, S.; White, R. D.; Lam, K. S.; Lebrilla, C. Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines Dis. Markers 2008, 25 (4-5) 243-258
11. Tretter, V.; Altmann, F.; Marz, L. Peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase F cannot release glycans with fucose attached alpha 1-3 to the asparagine-linked N-acetylglucosamine residue Eur. J. Biochem. 1991, 199 (3) 647-652
12. Ge, Y.; Gibbs, B. F.; Masse, R. Complete Chemical and Enzymatic Treatment of Phosphorylated and Glycosylated Proteins on ProteinChip Arrays Anal. Chem. 2005, 77 (11) 3644-3650
13. Papac, D.; Briggs, J.; Chin, E.; Jones, A. A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis Glycobiology 1998, 8 (5) 445-454
14. Lancaster, K. S.; An, H. J.; Li, B. S.; Lebrilla, C. B. Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR mass spectrometry Anal. Chem. 2006, 78 (14) 4990-4997
15. Bereman, M. S.; Young, D. D.; Deiters, A.; Muddiman, D. C. Development of a robust and high throughput method for profiling N-linked glycans derived from plasma glycoproteins by NanoLC-FTICR mass spectrometry J. Proteome Res. 2009, 8 (7) 3764-3770
16. Kyselova, Z.; Mechref, Y.; Kang, P.; Goetz, J. A.; Dobrolecki, L. E.; Sledge, G. W.; Schnaper, L.; Hickey, R. J.; Malkas, L. H.; Novotny, M. V. Breast cancer diagnosis and prognosis through quantitative measurements of serum glycan profiles Clin. Chem. 2008, 54 (7) 1166-1175
17. Palm, A. K.; Novotny, M. V. A monolithic PNGase F enzyme microreactor enabling glycan mass mapping of glycoproteins by mass spectrometry Rapid Commun. Mass Spectrom. 2005, 19 (12) 1730-1738
18. Szabo, Z.; Guttman, A.; Karger, B. L. Rapid release of N-linked glycans from glycoproteins by pressure-cycling technology Anal. Chem. 2010, 82 (6) 2588-2593
19. Sandoval, W. N.; Arellano, F.; Arnott, D.; Raab, H.; Vandlen, R.; Lill, J. R. Rapid removal of N-linked oligosaccharides using microwave assisted enzyme catalyzed deglycosylation Int. J. Mass Spectrom. 2007, 259 (1-3) 117-123
20. Bereman, M. S.; Williams, T. I.; Muddiman, D. C. Development of a nanoLC LTQ Orbitrap Mass Spectrometric Method for Profiling Glycans Derived from Plasma from Healthy, Benign Tumor Control, and Epithelial Ovarian Cancer Patients Anal. Chem. 2009, 81 (3) 1130-1136
21. Kronewitter, S. R.; Joo An, H.; Lorna de Leoz, M.; Lebrilla, C. B.; Miyamoto, S.; Leiserowitz, G. S. The development of retrosynthetic glycan libraries to profile and classify the human serum N-linked glycome PROTEOMICS 2009, 9 (11) 2986-2994