Catabolic function of compartmentalized alanine dehydrogenase in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

Journal of Bacteriology

Volumn 192, Issue 19, 2010, Pages 5165-5172

  Pernil, Rafael ^a,b   Herrero, Antonia ^a   Flores, Enrique ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE DEHYDROGENASE; ALANINE; BACTERIAL PROTEIN; PYRUVIC ACID;

AMINO TERMINAL SEQUENCE; ANABAENA; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL STRAIN; CATABOLISM; ENZYME ACTIVITY; GENE EXPRESSION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; CONFOCAL MICROSCOPY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM;

ANABAENA; ANABAENA SP.; CYANOBACTERIA;

ALANINE; ALANINE DEHYDROGENASE; ANABAENA; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MICROSCOPY, CONFOCAL; PYRUVIC ACID;

EID: 77957331323     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00603-10     Document Type: Article

References (39)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 2010. Current protocols in molecular biology. Wiley Interscience, New York, NY.
2. Cai, Y. P., and C. P. Wolk. 1990. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J. Bacteriol. 172:3138-3145.
3. Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38. (Pubitemid 26239677)
4. Curatti, L., E. Flores, and G. Salerno. 2002. Sucrose is involved in the diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp. FEBS Lett. 513:175-178.
5. Ehira, S., and M. Ohmori. 2006. NrrA, a nitrogen-responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 59:1692-1703.
6. Elhai, J., A. Vepritskiy, A. M. Muro-Pastor, E. Flores, and C. P. Wolk. 1997. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. J. Bacteriol. 179:1998-2005.
7. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
8. Flores, E., and A. Herrero. 1994. Assimilatory nitrogen metabolism and its regulation, p. 487-517. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, Netherlands.
9. Flores, E., and A. Herrero. 2010. Compartmentalized function through cell differentiation in filamentous cyanobacteria. Nat. Rev. Microbiol. 8:39-50.
10. Flores, E., A. Herrero, C. P. Wolk, and I. Maldener. 2006. Is the periplasm continuous in filamentous multicellular cyanobacteria? Trends Microbiol. 14:439-443.
11. Flores, E., R. Pernil, A. M. Muro-Pastor, V. Mariscal, I. Maldener, S. Lechno-Yossef, Q. Fan, C. P. Wolk, and A. Herrero. 2007. Septum-localized protein required for filament integrity and diazotrophy in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 189:3884-3890.
12. Herrero, A., A. M. Muro-Pastor, A. Valladares, and E. Flores. 2004. Cellular differentiation and the NtcA transcription factor in filamentous cyanobacteria. FEMS Microbiol. Rev. 28:469-487.
13. 14C-labeled metabolites in heterocysts and vegetative cells of Anabaena cylindrica filaments and their presumptive function as transport vehicles of organic carbon and nitrogen. J. Bacteriol. 155:628-633.
14. Kaneko, T., Y. Nakamura, C. P. Wolk, T. Kuritz, S. Sasamoto, A. Watanabe, M. Iriguchi, A. Ishikawa, K. Kawashima, T. Kimura, Y. Kishida, M. Kohara, M. Matsumoto, A. Matsuno, A. Muraki, N. Nakazaki, S. Shimpo, M. Sugimoto, M. Takazawa, M. Yamada, M. Yasuda, and S. Tabata. 2001. Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 8:205-213.
15. Kumar, K., R. A. Mella-Herrera, and J. W. Golden. 2010. Cyanobacterial heterocysts. Cold Spring Harb. Perspect. Biol. 2:a000315.
16. Labarre, J., P. Thuriaux, and F. Chauvat. 1987. Genetic analysis of amino acid transport in the facultatively heterotrophic cyanobacterium Synechocystis sp. strain 6803. J. Bacteriol. 169:4668-4673.
17. Lahmi, R., E. Sendersky, A. Perelman, M. Hagemann, K. Forchhammer, and R. Schwarz. 2006. Alanine dehydrogenase activity is required for adequate progression of phycobilisome degradation during nitrogen starvation in Synechococcus elongatus PCC 7942. J. Bacteriol. 188:5258-5265.
18. Mackinney, G. 1941. Absorption of light by chlorophyll solutions. J. Biol. Chem. 140:109-112.
19. Mariscal, V., A. Herrero, and E. Flores. 2007. Continuous periplasm in a filamentous, heterocyst-forming cyanobacterium. Mol. Microbiol. 65:1139-1145.
20. Markwell, M. A. K., S. M. Hass, L. L. Bieber, and N. E. Tolbert. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87:206-210.
21. Martín-Figueroa, E., F. Navarro, and F. J. Florencio. 2000. The GS-GOGAT pathway is not operative in the heterocysts. Cloning and expression of glsF gene from the cyanobacterium Anabaena sp. PCC 7120. FEBS Lett. 76:282-286.
22. Merino-Puerto, V., V. Mariscal, C. W. Mullineaux, A. Herrero, and E. Flores. 2010. Fra proteins influencing filament integrity, diazotrophy and localization of septal protein SepJ in the heterocyst-forming cyanobacterium Anabaena sp. Mol. Microbiol. 75:1159-1170.
23. Montesinos, M. L., A. Herrero, and E. Flores. 1995. Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 177:3150-3157.
24. Montesinos, M. L., A. Herrero, and E. Flores. 1997. Amino acid transport in taxonomically diverse cyanobacteria and identification of two genes encoding elements of a neutral amino acid permease putatively involved in recapture of leaked hydrophobic amino acids. J. Bacteriol. 179:853-862.
25. Mullineaux, C. W., V. Mariscal, A. Nenninger, H. Khanum, A. Herrero, E. Flores, and D. G. Adams. 2008. Mechanism of intercellular molecular exchange in heterocyst-forming cyanobacteria. EMBO J. 27:1299-1308.
26. Neilson, A. H., and M. Doudoroff. 1973. Ammonia assimilation in blue-green algae. Arch. Mikrobiol. 89:15-22.
27. Nicolaisen, K., V. Mariscal, R. Bredemeier, R. Pernil, S. Moslavac, R. López-Igual, I. Maldener, A. Herrero, E. Schleiff, and E. Flores. 2009. The outer membrane of a heterocyst-forming cyanobacterium is a permeability barrier for uptake of metabolites that are exchanged between cells. Mol. Microbiol. 74:58-70.
28. Olmedo-Verd, E., A. M. Muro-Pastor, E. Flores, and A. Herrero. 2006. Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120. J. Bacteriol. 188:6694-6699.
29. Pernil, R., S. Picossi, V. Mariscal, A. Herrero, and E. Flores. 2008. ABC-type amino acid uptake transporters Bgt and N-II of Anabaena sp. strain PCC 7120 share an ATPase subunit and are expressed in vegetative cells and heterocysts. Mol. Microbiol. 67:1067-1080.
30. Picossi, S., M. L. Montesinos, R. Pernil, C. Lichtlé, A. Herrero, and E. Flores. 2005. ABC-type neutral amino acid permease N-I is required for optimal diazotrophic growth and is repressed in the heterocysts of Anabaena sp. strain PCC 7120. Mol. Microbiol. 57:1582-1592.
31. Quintero, M. J., A. M. Muro-Pastor, A. Herrero, and E. Flores. 2000. Arginine catabolism in the cyanobacterium Synechocystis sp. strain PCC 6803 involves the urea cycle and arginase pathway. J. Bacteriol. 182:1008-1015.
32. Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.
33. 2-fixing blue-green alga, Anabaena cylindrica. Arch. Microbiol. 107:115-124.
34. Schilling, N., and K. Ehrnsperger. 1985. Cellular differentiation of sucrose metabolism in Anabaena variabilis. Z. Naturforsch. 40c:776-779.
35. Smith, A. J., J. London, and R. Y. Stanier. 1967. Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J. Bacteriol. 94:972-983.
36. 14C]glutamate by heterocysts isolated from Anabaena cylindrica. J. Bacteriol. 129:1545-1555.
37. 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica. J. Biol. Chem. 251:5027-5034.
38. Wolk, C. P., A. Ernst, and J. Elhai. 1994. Heterocyst metabolism and development, p. 769-823. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, Netherlands.
39. Xu, X., J. Elhai, and C. P. Wolk. 2008. Transcriptional and developmental responses by Anabaena to deprivation of fixed nitrogen, p. 383-422. In A. Herrero and E. Flores (ed.), The cyanobacteria: molecular biology, genomics and evolution. Caister Academic Press, Norfolk, United Kingdom.