Identification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans

Journal of Bacteriology

Volumn 192, Issue 19, 2010, Pages 5237-5244

  Simeonova, Diliana Dancheva ^a   Wilson, Marlena Marie ^b   Metcalf, William W ^b   Schink, Bernhard ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHITE; BACTERIAL PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CLONING VECTOR; CONTROLLED STUDY; DESULFOTIGNUM PHOSPHITOXIDANS; ELECTRON; GENE CLUSTER; GENE EXPRESSION; GENE LIBRARY; GRAM NEGATIVE BACTERIUM; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; NONHUMAN; OPERON; OXIDATION; PRIORITY JOURNAL; PTDFCG GENE; PTXD GENE; SEQUENCE ANALYSIS; BIOLOGICAL MODEL; DELTAPROTEOBACTERIA; GENETICS; METABOLISM; MOLECULAR GENETICS; MULTIGENE FAMILY; MUTATION; OXIDATION REDUCTION REACTION; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSPOSON;

DESULFOTIGNUM BALTICUM; DESULFOTIGNUM PHOSPHITOXIDANS; NEGIBACTERIA;

BACTERIAL PROTEINS; DELTAPROTEOBACTERIA; DNA TRANSPOSABLE ELEMENTS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; MUTATION; OXIDATION-REDUCTION; PHOSPHITES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 77957327191     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00541-10     Document Type: Article

References (34)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Ambudkar, S. V., V. Anantharam, and P. C. Maloney. 1990. UhpT, the sugar phosphate antiporter of Escherichia coli, functions as a monomer. J. Biol. Chem. 265:12287-12292.
3. Bates, P. 1987. Double cos site vectors: simplified cosmid cloning. Methods Enzymol. 153:82-94.
4. Bendtsen, J. D., H. Nielsen, G. von Heijne, and S. Bru. 2004. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340:783-795.
5. Casida, L. E., Jr. 1960. Microbial oxidation and utilization of orthophosphite during growth. J. Bacteriol. 80:237-241.
6. Eliot, A. C., B. M. Griffin, P. M. Thomas, T. W. Johannes, N. L. Kelleher, H. Zhao, and W. W. Metcalf. 2008. Cloning, expression, and biochemical characterization of Streptomyces rubellomurinus genes for biosynthesis of antimalarial compound FR900098. Chem. Biol. 15:765-770.
7. Foster, T. L., L. Winans, Jr., and S. J. Helms. 1978. Anaerobic utilization of phosphite and hypophosphite by Bacillus sp. Appl. Environ. Microbiol. 35:937-944.
8. Garcia Costas, A. M., A. K. White, and W. W. Metcalf. 2001. Purification and characterization of a novel phosphorus-oxidizing enzyme from Pseudomonas stutzeri WM88. J. Biol. Chem. 276:17429-17436.
9. Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
10. Hofmann, K., and W. Stoffel. 1993. TMbase-a database of membrane spanning protein segments. Biol. Chem. Hoppe-Seyler 374:166.
11. Island, M. D., and R. J. Kadner. 1993. Interplay between the membrane-associated UhpB and UhpC regulatory proteins. J. Bacteriol. 175:5028-5034.
12. Kovach, M. E., P. H. Elzer, D. S. Hill, G. T. Robertson, M. A. Farris, R. M. Roop II, and K. M. Peterson. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
13. Kuever, J., M. Konneke, A. Galushko, and O. Drzyzga. 2001. Reclassification of Desulfobacterium phenolicum as Desulfobacula phenolica comb. nov. and description of strain Sax(T) as Desulfotignum balticum gen. nov., sp. nov. Int. J. Syst. Evol. Microbiol. 51:171-177.
14. Lemieux, M. J., Y. Huang, and D.-N. Wang. 2004. Glycerol-3-phosphate transporter of Escherichia coli: structure, function and regulation. Res. Microbiol. 155:623-629.
15. Malacinski, G., and W. A. Konetzka. 1967. Orthophosphite-nicotinamide adenine dinucleotide oxidoreductase from Pseudomonas fluorescens. J. Bacteriol. 93:1906-1910.
16. Metcalf, W. W., and B. L. Wanner. 1991. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J. Bacteriol. 173:587-600.
17. Metcalf, W. W., and R. S. Wolfe. 1998. Molecular genetic analysis of phosphite and hypophpsphite oxidation by Pseudomonas stutzeri WM88. J. Bacteriol. 180:5547-5558.
18. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6. (Pubitemid 27073346)
19. Nyström, T., and F. C. Neidhardt. 1992. Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Mol. Microbiol. 6:3187-3198.
20. Nyström, T., and F. C. Neidhardt. 1996. Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12. J. Bacteriol. 178:927-930.
21. Pao, S. S., I. T. Paulsen, and M. H. Saier, Jr. 1998. Major facilitator superfamily. Microbiol. Mol. Biol. Rev. 62:1-34.
22. Sambrook, J., and D. W. Russell. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Schink, B. 2005. Biological cycling of phosphorus. In A. Sigel, H. Sigel, and R. K. O. Sigel (ed.), Metal ions in biological systems, vol. 43. Marcel Dekker Inc., New York, NY.
24. Schink, B., and M. Friedrich. 2000. Phosphite oxidation by sulphate reduction. Nature 406:37.
25. Schink, B., V. Thiemann, H. Laue, and M. W. Friedrich. 2002. Desulfotignum phosphitoxidans sp. nov., a new marine sulfate reducer that oxidizes phosphite to phosphate. Arch. Microbiol. 177:381-391.
26. Simeonova, D. D., I. Susnea, A. Moise, B. Schink, and M. Przybylski. 2009. "Unknown genome" proteomics: a new NAD(P)-dependent epimerase/dehydratase revealed by N-terminal sequencing, inverted PCR, and high resolution mass spectrometry. Mol. Cell. Proteomics 8:122-131.
27. von Heijne, G. 1992. Membrane protein structure prediction: hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225:487-494.
28. White, A. K., and W. W. Metcalf. 2004. The htx and ptx operons of Pseudomonas stutzeri WM88 are new members of the Pho regulon. J. Bacteriol. 186:5876-5882.
29. White, A. K., and W. W. Metcalf. 2004. Two C-P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphite, and hypophosphite. J. Bacteriol. 186:4730-4739.
30. Widdel, F., and N. Pfennig. 1981. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of a new sulfate-reducer enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov. sp. nov. Arch. Microbiol. 129:395-400.
31. Wilson, M. M., and W. W. Metcalf. 2005. Genetic diversity and horizontal transfer of genes involved in oxidation of reduced phosphorus compounds by Alcaligenes faecalis WM2027. Appl. Environ. Microbiol. 71:290-296.
32. Woodyer, R., W. A. van der Donk, and H. Zhao. 2003. Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design. Biochemistry 42:11604-11614. (Pubitemid 37243620)
33. Woodyer, R., J. L. Wheatley, H. A. Relyea, S. Rimkus, and W. A. van der Donk. 2005. Site-directed mutagenesis of active site residues of phosphite dehydrogenase. Biochemistry 44:4765-4774. (Pubitemid 40396755)
34. Yang, K., and W. W. Metcalf. 2004. A new activity for an old enzyme: Escherichia coli bacterial alkaline phosphatase is a phosphite-dependent hydrogenase. Proc. Nat. Acad. Sci. 101:7919-7924.