Identification of chlamydial species in crocodiles and chickens by PCR-HRM curve analysis

Veterinary Microbiology

Volumn 145, Issue 3-4, 2010, Pages 373-379

  Robertson, T ^a   Bibby, S ^a   O'Rourke, D ^a   Belfiore, T ^a   Agnew Crumpton, R ^a   Noormohammadi, A H ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

16S rRNA gene; Chlamydia Chlamydophila; Chlamydiaceae; HRM curve analysis; OmpA gene; Species identification

Indexed keywords

BACTERIAL DNA; NUCLEOTIDE;

ALLIGATOR; AMPLICON; ANALYZER; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CHICKEN; CHLAMYDIA; CHLAMYDOPHILA PSITTACI; GENE SEQUENCE; HIGH RESOLUTION MELT CURVE; NONHUMAN; POLYMERASE CHAIN REACTION; SPECIES DIFFERENTIATION;

ALLIGATORS AND CROCODILES; ANIMALS; CHICKENS; CHLAMYDIACEAE; CHLAMYDIACEAE INFECTIONS; DNA, BACTERIAL; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; TRANSITION TEMPERATURE;

ANIMALIA; CHLAMYDIACEAE; CHLAMYDOPHILA; CHLAMYDOPHILA PSITTACI; CROCODYLIDAE (ALL CROCODILES); GALLUS GALLUS; PARACHLAMYDIA;

EID: 77957283010     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2010.04.007     Document Type: Article

References (17)

1. Andersen A.A., Vanrompay D. Avian Chlamydiosis (psittacosis, ornithosis). Diseases of Poultry 2003, 863-879. Wiley/Blackwell. Y.M. Saif (Ed.).
2. Arzey G.G., Arzey K.E. Chlamydiosis in layer chickens. Aust. Vet. J. 1990, 67:461.
3. Barr D.A., Scott P.C., O'Rourke M.D., Coulter R.J. Isolation of Chlamydia psittaci from commercial broiler chickens. Aust. Vet. J. 1986, 63:377-378.
4. Bodetti T.J., Jacobson E., Wan C., Hafner L., Pospischil A., Rose K., Timms P. Molecular evidence to support the expansion of the hostrange of Chlamydophila pneumoniae to include reptiles as well as humans, horses, koalas and amphibians. Syst. Appl. Microbiol. 2002, 25:146-152.
5. Bodetti T.J., Viggers K., Warren K., Swan R., Conaghty S., Sims C., Timms P. Wide range of Chlamydiales types detected in native Australian mammals. Vet. Microbiol. 2003, 96:177-187.
6. Clarridge J.E. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev. 2004, 17:840-862.
7. Corsaro D., Greub G. Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria. Clin. Microbiol. Rev. 2006, 19:283-297.
8. Corsaro D., Venditti D. Diversity of the Parachlamydiae in the environment. Crit. Rev. Microbiol. 2006, 32:185-199.
9. Everett K.D., Bush R.M., Andersen A.A. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 1999, 49(Pt 2):415-440.
10. Hewson K., Noormohammadi A.H., Devlin J.M., Mardani K., Ignjatovic J. Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model. Arch. Virol. 2009, 154:649-660.
11. Kaltenboeck B., Kousoulas K.G., Storz J. Structures of and allelic diversity and relationships among the major outer membrane protein (ompA) genes of the four chlamydial species. J. Bacteriol. 1993, 175:487-502.
12. Laroucau K., Vorimore F., Aaziz R., Berndt A., Schubert E., Sachse K. Isolation of a new chlamydial agent from infected domestic poultry coincided with cases of atypical pneumonia among slaughterhouse workers in France. Infect. Genet. Evol. 2009, 9:1240-1247.
13. Mohan K., Dziva F., Mukarati N.L., Moyo D. Possible new Chlamydophila species causing chlamydiosis in farmed Nile crocodiles (Crocodylus niloticus). Vet. Rec. 2005, 157:23-25.
14. Robertson T., Bibby S., O'Rourke D., Belfiore T., Lambie H., Noormohammadi A.H. Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene. J. Appl. Microbiol. 2009, 107:2017-2028.
15. Soldati G., Lu Z.H., Vaughan L., Polkinghorne A., Zimmermann D.R., Huder J.B., Pospischil A. Detection of mycobacteria and chlamydiae in granulomatous inflammation of reptiles: a retrospective study. Vet. Pathol. 2004, 41:388-397.
16. Steer P.A., Kirkpatrick N.C., O'Rourke D., Noormohammadi A.H. Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region. J. Clin. Microbiol. 2009, 47:311-321.
17. Woldehiwet Z. Avian Chlamydophilosis (Chlamydiosis, psittacosis, ornithosis). Poultry Diseases 2008, 235-242. Saunders/Elsevier. M. Pattison, P.F. McMullin, J.M. Bradbury, D. Alexander (Eds.).