Testosterone-induced hypertrophy of L6 myoblasts is dependent upon Erk and mTOR

Biochemical and Biophysical Research Communications

Volumn 400, Issue 4, 2010, Pages 679-683

  Wu, Yong ^a   Bauman, William A ^a,b   Blitzer, Robert D ^b   Cardozo, Christopher ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Erk; Hypertrophy; MTOR; Muscle; Testosterone

Indexed keywords

MAMMALIAN TARGET OF RAPAMYCIN; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; SOMATOMEDIN C; TESTOSTERONE;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; HUMAN; IMMUNOPRECIPITATION; MUSCLE HYPERTROPHY; MYOBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; RAT; WESTERN BLOTTING;

ANIMALS; CELL ENLARGEMENT; CELL LINE; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; MUSCLE, SKELETAL; MYOBLASTS; PHOSPHORYLATION; RATS; RECEPTORS, ANDROGEN; TESTOSTERONE; TOR SERINE-THREONINE KINASES;

RATTUS;

EID: 77957281835     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.08.127     Document Type: Article

References (21)

1. Sinha-Hikim I., Artaza J., Woodhouse L., Gonzalez-Cadavid N., Singh A.B., Lee M.I., Storer T.W., Casaburi R., Shen R., Bhasin S. Testosterone-induced increase in muscle size in healthy young men is associated with muscle fiber hypertrophy. Am. J. Physiol. Endocrinol. Metab. 2002, 283:E154-E164.
2. Storer T.W., Magliano L., Woodhouse L., Lee M.L., Dzekov C., Dzekov J., Casaburi R., Bhasin S. Testosterone dose-dependently increases maximal voluntary strength and leg power, but does not affect fatigability or specific tension. J. Clin. Endocrinol. Metab. 2003, 88:1478-1485.
3. Bhasin S., Woodhouse L., Casaburi R., Singh A.B., Mac R.P., Lee M., Yarasheski K.E., Sinha-Hikim I., Dzekov C., Dzekov J., Magliano L., Storer T.W. Older men are as responsive as young men to the anabolic effects of graded doses of testosterone on the skeletal muscle. J. Clin. Endocrinol. Metab. 2005, 90:678-688.
4. Chen Y., Lee N.K., Zajac J.D., MacLean H.E. Generation and analysis of an androgen-responsive myoblast cell line indicates that androgens regulate myotube protein accretion. J. Endocrinol. Invest. 2008, 31:910-918.
5. Sarbassov D.D., Ali S.M., Sabatini D.M. Growing roles for the mTOR pathway. Curr. Opin. Cell Biol. 2005, 17:596-603.
6. Proud C.G. Signalling to translation: how signal transduction pathways control the protein synthetic machinery. Biochem. J. 2007, 403:217-234.
7. Xu T., Shen Y., Pink H., Triantafillou J., Stimpson S.A., Turnbull P., Han B. Phosphorylation of p70s6 kinase is implicated in androgen-induced levator ani muscle anabolism in castrated rats. J. Steroid Biochem. Mol. Biol. 2004, 92:447-454.
8. Bodine S.C., Stitt T.N., Gonzalez M., Kline W.O., Stover G.L., Bauerlein R., Zlotchenko E., Scrimgeour A., Lawrence J.C., Glass D.J., Yancopoulos G.D. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nat. Cell Biol. 2001, 3:1014-1019.
9. Rommel C., Bodine S.C., Clarke B.A., Rossman R., Nunez L., Stitt T.N., Yancopoulos G.D., Glass D.J. Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways. Nat. Cell Biol. 2001, 3:1009-1013.
10. Altamirano F., Oyarce C., Silva P., Toyos M., Wilson C., Lavandero S., Uhlen P., Estrada M. Testosterone induces cardiomyocyte hypertrophy through mammalian target of rapamycin complex 1 pathway. J. Endocrinol. 2009, 202:299-307.
11. Bhasin S., Woodhouse L., Casaburi R., Singh A.B., Bhasin D., Berman N., Chen X., Yarasheski K.E., Magliano L., Dzekov C., Dzekov J., Bross R., Phillips J., Sinha-Hikim I., Shen R., Storer T.W. Testosterone dose-response relationships in healthy young men. Am. J. Physiol. Endocrinol. Metab. 2001, 281:E1172-E1181.
12. Mauras N., Hayes V., Welch S., Rini A., Helgeson K., Dokler M., Veldhuis J.D., Urban R.J. Testosterone deficiency in young men: marked alterations in whole body protein kinetics, strength, and adiposity. J. Clin. Endocrinol. Metab. 1998, 83:1886-1892.
13. Urban R.J., Bodenburg Y.H., Gilkison C., Foxworth J., Coggan A.R., Wolfe R.R., Ferrando A. Testosterone administration to elderly men increases skeletal muscle strength and protein synthesis. Am. J. Physiol. 1995, 269:E820-E826.
14. Wu Y., Zhao W., Zhao J., Pan J., Wu Q., Zhang Y., Bauman W.A., Cardozo C.P. Identification of androgen response elements in the IGF-1 upstream promoter. Endocrinology 2007, 148:2984-2993.
15. Wu Y., Zhao W., Zhao J., Zhang Y., Qin W., Pan J., Bauman W.A., Blitzer R.D., Cardozo C. REDD1 is a major target of testosterone action in preventing dexamethasone-induced muscle loss. Endocrinology 2009.
16. Wu Y., Zhao W., Zhao J., Zhang Y., Qin W., Pan J., Bauman W.A., Blitzer R.D., Cardozo C. REDD1 is a major target of testosterone action in preventing dexamethasone-induced muscle loss. Endocrinology 2010, 151:1050-1059.
17. Gatson J.W., Kaur P., Singh M. Dihydrotestosterone differentially modulates the mitogen-activated protein kinase and the phosphoinositide 3-kinase/Akt pathways through the nuclear and novel membrane androgen receptor in C6 cells. Endocrinology 2006, 147:2028-2034.
18. Cheng J., Watkins S.C., Walker W.H. Testosterone activates mitogen-activated protein kinase via Src kinase and the epidermal growth factor receptor in sertoli cells. Endocrinology 2007, 148:2066-2074.
19. Estrada M., Espinosa A., Muller M., Jaimovich E. Testosterone stimulates intracellular calcium release and mitogen-activated protein kinases via a G protein-coupled receptor in skeletal muscle cells. Endocrinology 2003, 144:3586-3597.
20. Stahn C., Buttgereit F. Genomic and nongenomic effects of glucocorticoids. Nat. Clin. Pract. Rheumatol. 2008.
21. Tsokas P., Ma T., Iyengar R., Landau E.M., Blitzer R.D. Mitogen-activated protein kinase upregulates the dendritic translation machinery in long-term potentiation by controlling the mammalian target of rapamycin pathway. J. Neurosci. 2007, 27:5885-5894.