A new multiplex PCR method for rapid detection of genes encoding VIM and IMP types of metallo beta lactamases;VIM ve IMP tipi metallo beta laktamaz genlerinin hızlı saptanması i̇çin yeni bir multipleks PZR metodu

Turkiye Klinikleri Journal of Medical Sciences

Volumn 30, Issue 4, 2010, Pages 1312-1316

  Küçükbasmaci, Ömer ^a   Midilli, Kenan ^a   Issa, Ghassan ^b   Güven, Özlem ^a   Gönüllü, Nevriye ^a  

^a ISTANBUL UNIVERSITY   (Turkey)

^b ISTANBUL UNIVERSITY   (Turkey)

Author keywords

Beta lactamases; Carbapenems; Polymerase chain reaction; Pseudomonas aeruginosa

Indexed keywords

CEFEPIME; CEFTAZIDIME; IMIPENEM; MEROPENEM; METALLO BETA LACTAMASE; METALLO BETA LACTAMASE IMP; METALLO BETA LACTAMASE IMP 1; METALLO BETA LACTAMASE IMP 13; METALLO BETA LACTAMASE IMP 2; METALLO BETA LACTAMASE VIM; METALLO BETA LACTAMASE VIM 1; METALLO BETA LACTAMASE VIM 2; METALLO BETA LACTAMASE VIM 4; METALLO BETA LACTAMASE VIM 5; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; GENE IDENTIFICATION; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PSEUDOMONAS AERUGINOSA; TURKEY (REPUBLIC); UNIVERSITY HOSPITAL;

EID: 77957243471     PISSN: 13000292     EISSN: None     Source Type: Journal    
DOI: 10.5336/medsci.2009-11518     Document Type: Article

References (13)

1. Walsh TR. Clinically significant carbapenemases: an update. Curr Opin Infect Dis 2008;21(4):367-371.
2. Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Testing. 17th informational supplement (M100-S17). Wayne, PA: CLSI; 2007. p.38-39.
3. Walsh TR, Bolmström A, Qwarnstrom A. Gales A. Evaluation of a new Etest for detecting metallo-beta-lactamases in routine clinical testing. J Clin Microbiol 2002;40(8):2755-2759.
4. Queenan AM, Bush K Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 2007;20(3):440-582.
5. Aktas Z, Bal C, Midilli K, Poirel L, Nordmann P. First IMP-1-producing Klebsiella pneumoniae isolate in Turkey. Clin Microbiol Infect 2006;12(7):695-696.
6. Yildirim I, Ceyhan M, Gur D, Mugnaioli C. Rossolini GM. First detection of VIM-1 type metallo-beta-lactamase in a multidrug-resistant Klebsiella pneumoniae clinical isolate from Turkey also producing the CTX-M-15 extended-spectrum beta-lactamase. J Chemother 2007;19(4):467-468.
7. Aktaş Z, Kayacan CB. Investigation of metallo-beta-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy and PCR. Scand J Infect Dis 2008;40(4):320-325.
8. Ozgumus OB, Caylan R, Tosun I, Sandalli C, Aydin K, Koksal I. Molecular epidemiology of clinical Pseudomonas aeruginosa isolates carrying IMP-1 metallo-beta-lactamase gene in a University Hospital in Turkey. Microb Drug Resist 2007;13(3):191-198.
9. Bahar G, Mazzariol A, Koncan R, Mert A, Fon-tana R, Rossolini GM, et al. Detection of VIM-5 metall o-beta-lactamase in a Pseudomonas aeruginosa clinical isolate from Turkey. J An-timicrob Chemother 2004;54(1):282-283.
10. Yakupogullari Y, Poirel L, Bernabeu S, Kizirgil A, Nordmann P. Multidrug-resistant Pseudomonas aeruginosa isolate co-expressing extended-spectrum beta-lactamase PER-1 and metallo-beta-lactamase VIM-2 from Turkey. J Antimicrob Chemother 2008;61 (1):221-222.
11. Gacar GG, Midilli K, Kolayli F, Ergen K, Gun-des S, Hosoglu S, et al. Genetic and enzymatic properties of metallo-beta-lactamase VIM-5 from a clinical isolate of Enterobacter cloacae. Antimicrob Agents Chemother 2005;49(10):4400-4403.
12. Mendes RE, Kiyota KA, Monteiro J, Castan-heira M, Andrade SS, Gales AC, et al. Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex realtime PCR assay and melt curve analysis. J Clin Microbiol 2007;45(2):544-547.
13. Bisiklis A, Papageorgiou F, Frantzidou F, Alex-iou-Daniel S. Specific detection of blaVIM and blaIMP metallo-beta-lactamase genes in a single real-time PCR. Clin Microbiol Infect 2007;13(12):1201-1203.