Novel electron tomographic methods to study the morphology of keratin filament networks

Microscopy and Microanalysis

Volumn 16, Issue 4, 2010, Pages 462-471

  Sailer, Michaela ^a   Höhn, Katharina ^a   Lück, Sebastian ^a   Schmidt, Volker ^a   Beil, Michael ^b   Walther, Paul ^a  

^a UNIVERSITY OF ULM   (Germany)

^b UNIVERSITY HOSPITAL ULM   (Germany)

Author keywords

actin; electron microscopy; keratin; keratin (intermediate) filaments; pancreas; SEM tomography; STEM tomography

Indexed keywords

KERATIN; MACROMOLECULE;

CONFERENCE PAPER; ELECTRON TOMOGRAPHY; HUMAN; MACROMOLECULE; METHODOLOGY; PANCREAS; TUMOR CELL LINE; ULTRASTRUCTURE; PROCEDURES;

CELL LINE, TUMOR; ELECTRON MICROSCOPE TOMOGRAPHY; HUMANS; KERATINS; MACROMOLECULAR SUBSTANCES; PANCREAS;

EID: 77957236623     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927610093657     Document Type: Conference Paper

References (40)

1. ALBERTS, B., JOHNSON, A., LEWIS, L., RAFF, M., ROBERTS, K. & WALTER, P. (2008). Molecular Biology of the Cell, 5th Ed. New York: Garland Publishing.
2. BAUMEISTER, W. (2004). Mapping molecular landscapes inside cells. Biol Chem 385, 865-872. (Pubitemid 39481133)
3. BEIL, M., BRAXMEIER, H., FLEISCHER, F., SCHMIDT, V. & WALTHER, P. (2005). Quantitative analysis of keratin filament networks in scanning electron microscopy images of cancer cells. J Micros 220, 84-95. (Pubitemid 41746538)
4. BEIL, M., ECKEL, S., FLEISCHER, F., SCHMIDT, H., SCHMIDT, V. & WALTHER, P. (2006). Fitting of random tessellation models to keratin filament networks. J Theor Biol 241, 62-72. (Pubitemid 43817988)
5. BEIL, M., MICOULET, A., VON WICHERT, G., PASCHKE, S., WALTHER, P., OMARY, M.B., VAN VELDHOVEN, P.P., GERN, U., WOLFF-HIEBER, E., EGGERMANN, J., WALTENBERGER, J., ADLER, G., SPATZ, J. & SEUFFERLEIN, T. (2003). Sphingosylphosphorylcho-line regulates keratin network architecture and visco-elastic properties of human cancer cells. Nat Cell Biol 5, 803-811. (Pubitemid 37087168)
6. BUSER, C. & WALTHER, P. (2008). Freeze-substitution: The addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 °C. J Mircrosc 230, 268-277. (Pubitemid 351594966)
7. BUZUG, T.M. (2008). Computed Tomography. Berlin: Springer.
8. COULOMBE, PA. & OMARY, M.B. (2002). "Hard" and "soft" principles defining the structure, function and regulation of keratin intermediate filaments. Curr Opin Cell Biol 14, 110-122.
9. DUBOCHET, J. (2007). The physics of rapid cooling and its implications for cryoimmobilization of cells. Methods Cell Biol 79, 7-21.
10. ECHLIN, P. (1992). Low-Temperature Microscopy and Analysis. New York, London: Plenum Press.
11. FUCHS, E. & WEBER, K (1994). Keratin (intermediate) filaments: Structure, dynamics, function and disease. Annu Rev Biochem 63, 345-382.
12. GOLDSTEIN, J., NEWBURY, D., JOY, D., LYMAN, C., ECHLIN, P., LIFSHIN, E., SAWYER, L. & MICHAEL, J. (2007). Scanning Electron Microscopy and X-Ray Microanalysis, 3rd Ed. New York: Springer.
13. HATZFELD, M. & FRANKE, WW. (1985). Pair formation and promiscuity of cytokeratins: Formation in vitro of heterotypic complexes and keratin (intermediate)-sized filaments by homologous and heterologous recombinations of purified polypeptides. J Cell Biol 101, 1826-1841. (Pubitemid 16192156)
14. HAWKES, P.W. (2005). The electron microscope as a structure projector. In Electron Tomography, 2nd Ed., Frank, J. (Ed.), pp. 83-107. New York: Springer.
15. HERRMANN, H., HESSE, M., REICHENZELLER, M., AEBI, U. & MA-GIN, T.M. (2003). Functional complexity of keratin (intermediate) filament cytoskeletons: From structure to assembly to gene ablation. Int Rev Cytol 223, 83-175. (Pubitemid 36278512)
16. HOHENBERG, H.H., MÜLLER-REICHERT, T, SCHWARZ, H. & ZIEROLD, K. (2003). Foreword, special issue on high pressure freezing. J Microsc 212, 1-2.
17. HÖHN, K., PUSAPATI, G.V., SEUFFERLEIN, T, ADLER, G. & WALTHER, P. (2009). 3D reconstruction of Golgi stacks in Brefeldin A treated cells. First results using STEM tomography. In Proceedings of MC2009, vol.2, Life Science, Pabst, M.A. & Zellnig, G. (Eds.), pp. 43-44. Graz, Austria: Verlag der TU.
18. HOPPE, W., GASSMANN, J., HUNSMANN, N., SCHRAMM, H.J. & STURM, M. (1974). Three-dimensional reconstruction of individual negatively stained yeast fatty-acid synthetase molecules from tilt series in the electron microscope. Z Physiol Chem 355, 1483-1487.
19. HUMBEL, B.M. (2009). Freeze substitution. In Handbook of Cryo-Preparation Methods for Electron Microscopy, Cavalier, A., Speh-ner, D. & Humbel, B.M. (Eds.), pp. 319-341. Boca Raton, FL: CRC Press.
20. KREMER, J.R., MASTRONARDE, D.N. & MCINTOSH, J.R. (1996). Computer visualization of three-dimensional image data using IMOD. J Struct Biol 116, 71-76. (Pubitemid 26093126)
21. LAUTENSCHLÄGER, F., PASCHKE, S., SCHINKINGER, S., BRUEL, A., BEIL, M. & GUCK, J. (2009). The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc Natl Acad Sci USA 106, 15696-15701.
22. LÜCK, S., SAILER, M., SCHMIDT, V. & WALTHER, P. (2010). Three-dimensional analysis of intermediate filament networks using SEM tomography. J Microsc 236, 1-16.
23. MARTI, O., HOLZWARTH, M. & BEIL, M. (2008). Measuring the nanomechanical properties of cancer cells by digital pulsed force mode imaging. Nanotechnology 19, 384015.
24. MIDGLEY, P.A. & DUNIN-BORKOWSKI, R.E. (2009). Electron tomography and holography in materials science. Nat Mater 8, 271-280.
25. MIDGLEY, P.A., WEYLAND, M., THOMAS, J.M. & JOHNSON, B.F.G. (2001). Z-contrast tomography: A technique in 3-dimensional nanostructural analysis based on Rutherford scattering. Chem Comm 18, 907-908. (Pubitemid 32505765)
26. PAWLEY, J. (2008). LVSEM for biology. In Biological Low-Voltage Scanning Electron Microscopy, Schatten, H. & Pawley, J.B. (Eds.), pp. 27-106. New York: Springer.
27. RADON, J. (1917). Über die Bestimmung von Funktionen längs gewisser Mannigfaltigkeiten. Berichte der math.-phys. Kl. Säch-sischen Gesellschaft der Wissenschaften 59, 262-277.
28. RESCH, G.P., GOLDIE, K.N., KREBS, A., HOENGER, A. & SMALL, J.V. (2002). Visualisation of the actin cytoskeleton by cryo-electron microscopy. J Cell Sci 115, 1877-1882. (Pubitemid 34567249)
29. RIS, H. (1985). The cytoplasmic filament system in critical point-dried whole mounts and plastic-embedded sections. J Cell Biol 100, 1474-1487. (Pubitemid 15098022)
30. SAILER, M., LÜCK, S., SCHMIDT, V., BEIL, M., ADLER, G. & WALTHER, P. (2008). Three-dimensional analysis of the keratin (intermedi-ate) filament network using SEM-tomography. In Proceedings of EMC 2008, Aretz, A., Hermanns-Sachweh, B. & Mayer, J. (Eds.), pp. 91-92. Berlin, Heidelberg: Springer.
31. SAILER, M., LÜCK, S., SCHMIDT, V., BEIL, M., ADLER, G. & WALTHER, P. (2009). Three-dimensional analysis of the keratin (intermedi-ate) filament network using SEM-tomography. In Proceedings of MC2009, Pabst, M.A. & Zellnig, G. (Eds.), pp. LI.P603-LI.P604. Graz, Austria: Verlag der TU.
32. SEILER, H. (1967). Einige aktuelle Probleme der Sekundärelektronen- emission. Z Angew Phys 22, 249-263.
33. SVITKINA, T.M. (2007). Electron microscopic analysis of the leading edge in migrating cells. Methods Cell Biol 79, 295-319.
34. SVITKINA, T.M. & BORISY, G.G. (1998). Correlative light and electron microscopy of the cytoskeleton of cultured cells. Method Enzymol 298, 570-592.
35. VIGNAL, E. & RESCH, G. (2003). Shedding light and electrons on the lamellipodium: Imaging the motor of crawling cells. Biotech-niques 34, 780-784, 786, 788-789. (Pubitemid 36418338)
36. WALTHER, P. (2003). The potential of high resolution cryo-SEM in life science. Hitachi Instrument News 40, 3-8.
37. WALTHER, P. (2008). High-resolution cryo-SEM allows direct identification of F-actin at the inner nuclear membrane of Xenopus oocytes by virtue of its structural features. J Microsc 232, 379-385.
38. WALTHER, P. & ZIEGLER, A. (2002). Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water. J Microsc 208, 3-10. (Pubitemid 35365577)
39. WINDOFFER, R., WÖLL, S., STRNAD, P. & LEUBE, R.E. (2004). Identification of novel principles of keratin filament network turnover in living cells. Mol Biol Cell 5, 2436-2448. (Pubitemid 38580658)
40. YAKUSHEVSKA, A.E., LEBBINK, M.N., GEERTS, W.J., SPEK, L., VAN DONSELAAR, E.G., JANSEN, K.A., HUMBEL, B.M., POST, J.A., VERKLEIJ, A.J. & KOSTER, A.J. (2007). STEM tomography in cell biology. J Struct Biol 159, 381-391. (Pubitemid 47348299)