Molecular determinants of thermozyme activity and stability: Analysis of xylose isomerase and amylo-pullulanase

Progress in Biotechnology

Volumn 12, Issue C, 1996, Pages 145-161

  Gregory Zeikus, J ^a  

^a Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 77957202280     PISSN: 09210423     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0921-0423(96)80366-2     Document Type: Article

References (25)

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3. Zeikus J.G., Lee C., Lee Y.-E., and Saha B.C. Thermostable saccharidases: New sources, uses and biodesigns. In: Leatham G.F., and Himmel M.E. (Eds). ACS Symposium Series 460, Enzymes in Biomass Conversion (1991), American Chemical Society, Washington, D.C. 36-51
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5. Lowe S.E., Jain M.K., and Gregory Zeikus J. Biology, Ecology, and Biotechnological Applications of Anaerobic Bacteria Adapted to Environmental Stresses in Temperature, pH, Salinity, or Substrates. Microbiol. Rev. 57 (1993) 451-509
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14. Lee Y.-E., Lowe S.E., Henrissat B., and Zeikus J.G. Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI. J. of Bacteriol. 175 (1993) 5890-5898
15. Mathupala S.^P., Park J.-H., and Gregory Zeikus J. Evidence for α-1,6 and α-1,4-Glucosidic Bond Cleavage in Highly Branched Glycogen by Amylopullulanase from Thermoanaerobacter Ethanolicus. Biotechnol. Lett. 16 (1994) 1311-1316
16. Park, J.H., S. Mathupala, C. Peterson and J.G. Zeikus. Unpublished Findings.
17. Shaha B.C., Mathupala S., and Zeikus J.G. Purification and characterization of a highly thermostable pullulanase from Clostridium thermohydrosulfuricum. Biochem. J. 252 (1988) 343-348
18. Mathupala S., Saha B.C., and Zeikus J.G. Substrate competition and specificity at the active site of amylopullulanase from Clostridium thermohydrosulfuricum. Biochem. Biophys. Res. Comm. 166 (1990) 126-132
19. Mathupala S.P., Lowe S.E., Podkovyrov S.M., and Zeikus J.G. Sequencing of the Amylopullulanase (aup) Gene of Thermoanaerobacter ethanolicus 39E, and Identification of the Active Site by Site-directed Mutagenesis. J. Biol. Chem. 268 (1993) 16332-16344
20. Lee Y.-E., Ramesh Matur.V., and Zeikus J.G. Cloning, sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain. B6A-RI. J. Gen. Microbiol. 139 (1993) 1227-1234
21. Vieille C., Hess J.M., Kelly R.M., and Zeikus J.G. xylA Cloning and Sequencing and Biochemical Characterization of Xylose Isomerase from Thermotoga neapolitana. Appl. Environ. Microbiol. 61 (1995) 1867-1875
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23. Meng M., Michael B., and Zeikus J.G. The role of active-site aromatic and polar residues in catalysis and substrate discrimination by xylose isomerase. Proc. Natl. Acad. Sci. USA 90 (1993) 8459-8463
24. (Manuscript Submitted). Tchernenko V., Vieille C., Burdette D., and Zeikus J.G. Relationships between enzyme molecular rigidity, stability and activity: analysis of xylose isomerase thermal features. Appl. Biochem. and Biotech. (1996)
25. Meng M., Bagdasarian M., and Zeikus J.G. Thermal Stabilization of Xylose Isomerase from Thermoanaerobacterium thermosulfurigenes. Bio/Technology 11 (1993) 1157-1161