Preparation of Fluorescent Pre-mRNA Substrates for an smFRET Study of Pre-mRNA Splicing in Yeast

Methods in Enzymology

Volumn 472, Issue , 2010, Pages 31-40

  Abelson, John ^a   Hadjivassiliou, Haralambos ^a   Guthrie, Christine ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; RNA PRECURSOR; SACCHAROMYCES CEREVISIAE PROTEIN; UBC4 PROTEIN, S CEREVISIAE; UBIQUITIN CONJUGATING ENZYME;

ARTICLE; CHEMISTRY; CONFORMATION; EXON; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETICS; INSTRUMENTATION; INTRON; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; RNA SPLICING; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; EXONS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; INTRONS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID CONFORMATION; RNA PRECURSORS; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; UBIQUITIN-CONJUGATING ENZYMES;

MLCS; MLOWN;

EID: 77957066363     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/S0076-6879(10)72017-6     Document Type: Chapter

References (7)

1. Abelson, J., Blanco, M., Ditzler, M.A., Fuller, F., Aravamudhan, P., Wood, M., Villa, T., Ryan, D., Pleiss, J.A., Maeder, C., Guthrie, C., Walter, N.G., Conformational dynamics of single pre-mRNA molecules during spliceosome assembly and splicing. Nat. Struct. Mol. Biol. 17 (2010), 1767–1775.
2. Blanco, M., Walter, N.G., Analysis of complex single molecule FRET time trajectories. Methods Enzymol. 472 (2010), 153–178.
3. Ho, C.K., Shuman, S., Bacteriophage T4 RNA ligase 2 (gp24.1) exemplifies a family of RNA ligases found in all phylogenetic domains. Proc. Natl. Acad. Sci. USA 99 (2002), 12709–12714.
4. Lin, R.J., Newman, A.J., Cheng, S.C., Abelson, J., Yeast mRNA splicing in vitro. J. Biol. Chem. 260 (1985), 14780–14792.
5. Pleiss, J.A., Whitworth, G.B., Bergkessel, M., Guthrie, C., Transcript specificit in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components. PLoS Biol., 5, 2007, e90.
6. Stark, M.R., Pleiss, J.A., Deras, M., Scaringe, S.A., Rader, S.D., An RNA ligase-mediated method for the efficient creation of large, synthetic RNAs. RNA 12 (2006), 2014–2019.
7. Vijayraghavan, U., Company, M., Abelson, J., Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3 (1989), 1206–1216.