Development of a multiplex PCR for the simultaneous amplification and genotyping of glycoprotein N among human cytomegalovirus strains

New Microbiologica

Volumn 33, Issue 3, 2010, Pages 257-262

  Pignatelli, Sara ^a   Maurizio, Daniela ^a   Ladini, Maria Paola ^a   Monte, Paola Dal ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Gene polymorphisms; Glycoprotein n; Human cytomegalovirus; Multiplex pcr; Wild type strains

Indexed keywords

PRIMER DNA; VIRUS ENVELOPE PROTEIN;

ARTICLE; CLASSIFICATION; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; EVALUATION; GENETIC POLYMORPHISM; GENETICS; GENOTYPE; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; VIROLOGY; EVALUATION STUDY; PROCEDURES;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA PRIMERS; GENOTYPE; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; VIRAL ENVELOPE PROTEINS;

HUMAN HERPESVIRUS 5;

EID: 77957048079     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. ARAV-BOGER R., BATTAGLIA C.A., LAZZAROTTO T., GABRIELLI L., ZONG J.C., HAYWARD G.S., DIENERWEST M., LANDINI M.P. (2006). Cytomegalovirus (CMV)-encoded UL144 (truncated tumor necrosis factor receptor) and outcome of congenital CMV infection. J. Infect. Dis. 194, 464-473.
2. BEYARI M.M., HODGSON T.A., KONDOWE,W., MOLYNEUX E.M., SCULLY C., PORTER S.R., TEO C.G. (2005). Interand Intra- person cytomegalovirus infection in Malawian families. J. Med. Virol. 75, 575-582.
3. BURKHARDT C., HIMMELEIN S., BRITT W., WINKLER T., MACH M. (2009). Glycoprotein N subtypes of human cytomegalovirus induce a strain-specific antibody response during natural infection. J. Gen. Virol 90, 1951-1961.
4. CAPRETTI M.G., LANARI M., LAZZAROTTO T., GABRIELLI L., PIGNATELLI S., CORVAGUA L., TRIDAPALLI E., FALDELLA G. (2009). Very low birth weight infants born to cytomegalovirus-seropositive mothers fed with their mother's milk: a prospective study. J. Pediatr. 154, 842-848.
5. CHEN H.P., LIN J.C., YANG S.P., LAN Y.C., WENG W.S., TSAI C.H., HO D.M., LIU C.Y., CHO W.L., CHAN Y.J. (2008). The type-2 variant of human cytomegalovirus glycoprotein N (gN-2) is not the rarest in the Chinese population of Taiwan: influence of primer design. J. Virol. Meth. 151, 161-164.
6. DAL MONTE P., PIGNATELLI S., MACH M., M.P. LANDINI. (2001). The product of human cytomegalovirus (HCMV) UL73 is a new polymorphic structural glycoprotein (gpUL73). J. Hum. Virol. 4, 26-34.
7. DAL MONTE P., PIGNATELLI S., ROSSINI G., LANDINI M.P. (2004). Genomic variants among human cytomegalovirus (HCMV) clinical isolates: the glycoprotein n (gN) paradigm. Hum Immunol 65, 387-394.
8. ELNIFRO E.M., ASHSHI A.M., COOPER R.J., KLAPPER P.E. (2000). Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13, 559-570.
9. GAYTANT M.A., STEEGERS E.A., SEMMEKROT B.A., MERKUS H.M., GALAMA J.M. (2002). Congenital cytomegalovirus infection: review of the epidemiology and outcome. Obstet. Gynecol. Surv. 57, 245-256. (Pubitemid 34407101)
10. GLEAVES C.A., SMITH T.F., SHUSTER E.A., PEARSON G.R. (1984). Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19, 917-919.
11. Ho M. (1990). Epidemiology of cytomegalovirus infections. Rev. Infect. Dis. 12, S701.
12. KARI B., GEHRZ R. (1993). Structure, composition and heparin binding properties of a human cytomegalovirus glycoprotein complex designed gCII. J. Gen. Virol. 74, 225-264.
13. LANARI M., CAPRETTI M.G., LAZZAROTTO T., GABRIELLI L., PIGNATELLI S., DAL MONTE P., LANDINI M.P, FALDELLA G. (2008). Cytomegalovirus infection via mothers milk: could distinct virus strains determine different disease patterns in preterm twins? New Microbiol. 31, 131-135.
14. MACH M., KROPFE B., DAL MONTE P., BRITT W.J. (2000). Complex formation of human cytomegalovirus glycoprotein M (gpULlOO) and glycoprotein N (gpUL73J. J. Virol. 74, 11881-11892.
15. MATTICK C., DEWIN D., POLLEY S., SEVILLA-REYES E., PIGNATELLI S., RAWLINSON W., WILKINSON G., DAL MONTE P., GOMPELS U.A. (2004). Linkage of human cytomegalovirus glycoprotein gO variant groups identified from worldwide clinical isolates with gN genotypes, implications for disease associations and evidence for N-terminal sites of positive selection. Virology. 318, 582-597.
16. MEYER-KONIG U., VOGELBERG C., BONGARTS A., KAMPA o D., DELBRÜCK R., WOLFF-VORBECK G., KIRSTE G., HABERLAND M., HUFERT F.T., VON LAER D. (1998). Glycoprotein B genotype correlates with cell tropism in vivo of human cytomegalovirus infection. J. Med. Virol. 55, 75-81.
17. NOVAK Z., ROSS S.A., KUMAR PATRO R., KUMAR PATI S., KUMBLA R.A., BRICE S., BOPPANA S.B. (2008). Cytomegalovirus strain diversity in seropositive women. J. Clin. Microb. 46, 882-886.
18. PIGNATELLI S., DAL MONTE P., LANDINI M.P. (2001). gpUL73(gN) genomic variants of Human Cytomegalovirus (HCMV) isolates are clustered into four distinct genotypes. J. Gen. Virol. 82, 2777-2784.
19. PIGNATELLI S., DAL MONTE P., ROSSINI G., CHOU S., GOJOBORI T., HANADA K., GUO J.J., RAWLINSON W., BRITT W., MACH M., LANDINI M.P. (2003a). Human cytomegalovirus glycoprotein N (gpUL73-gN) genomic variants: identification of a novel subgroup, geographical distribution and evidence of positive selective pressure. J. Gen. Virol. 84, 647-655.
20. PIGNATELLI S., DAL MONTE P., ROSSINI G., GATTO M.R., LANDINI M.P. (2003b). Human cytomegalovirus glycoprotein N (gN) genotypes in AIDS patients. AIDS. 17, 761-763. (Pubitemid 36521086)
21. PIGNATELLI S., DAL MONTE P, ROSSINI G, LANDINI M.P. (2004). Genetic polymorphisms among human cytomegalovirus (HCMV) wild-type strains. Rev. Med. Virol. 14, 383-410.
22. PIGNATELLI S., DAL MONTE P., ROSSINI G., CAMOZZI D., TOSCANO V., CONTE R., LANDINI M.P. (2006). Latencyassociated human cytomegalovirus (HCMV) gN genotypes in monocytes from healthy blood donors. Transfusion. 46, 1754-1762.
23. PIGNATELLI S., DAL MONTE P. (2009). Epidemiology of human cytomegalovirus strains through comparison of methodological approaches to explore gN variants. New Microbiol. 32, 1-10.
24. PIGNATELLI S., LAZZAROTTO T., GATTO M.R., DAL MONTE P., LANDINI M.R, FALDELLA G., LANARI M. Cytomegalovirus gN genotypes distribution among congenitally infected newborns and their relationship with symptoms at birth and sequelae. Clin. Infect. Dis. 2010; 51 (1): 33-41.
25. PUCHHAMMER-STÖCKL E., GORZER I., ZOUFALY A., JAKSCH P., BAUER C.C., KLEPETKO W. POPOW-KRAUPP T. (2006). Emergence of multiple cytomegalovirus strains in blood and lung of lung transplant recipients. Transplantation. 81, 187-194.
26. ROSSINI G., PIGNATELLI S., DAL MONTE P., CAMOZZI D., LAZZAROTTO T., GABRIELLI L., GATTO M.R., LANDINI M.P. (2005). Monitoring for human cytomegalovirus infection in solid organ transplant recipients through antigenemia and glycoprotein N (gN) variants: evidence of correlation and potential prognostic value of gN genotypes. Microbes and infection. 7, 890-896.
27. SHEPP D.H., MATCH M.E., ASHRAF A.B., LIPSON S.M., MILLAN C., PERGOLIZZI R. (1998). Cytomegalovirus glycoprotein B groups associated with retinitis in AIDS. J. Inf. Dis. 174, 184-187.
28. YAN H., KOYANO S., INAMI Y., YAMAMOTO Y., SUZUTANI T., MIZUGUCHI M., USHIJIMA H., KURANE I., INOUE N. (2008). Genetic linkage among human cytomegalovirus glycoprotein N (gN) and gO genes, with evidence for recombination from congenitally and post-natally infected Japanese infants. J. Gen. Virol. 89, 2275-2279.