Contaminations of laboratory surfaces with Staphylococcus aureus are affected by the carrier status of laboratory staff

Journal of Applied Microbiology

Volumn 109, Issue 4, 2010, Pages 1284-1293

  Schmidlin, M ^a   Alt, M ^a   Vogel, G ^a   Voegeli, U ^a   Brodmann, P ^a   Bagutti, C ^a  

^a Biosafety Laboratory   (Switzerland)

Author keywords

Environmental; microbial contamination; polymerase chain reaction (PCR); randomly amplified polymorphic DNA (RAPD); Staphylococci

Indexed keywords

BACTERIA; CONTAMINATION; DNA; LABORATORIES;

ENVIRONMENTAL; MICRO-ORGANISMS; MICROBIAL CONTAMINATION; POLYMERASE CHAIN REACTION; RANDOMLY AMPLIFIED POLYMORPHIC DNA; S. AUREUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; WORKING ENVIRONMENT;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA;

AGAR; BACTERIUM; BIOSAFETY; DNA; LABORATORY METHOD; POLLUTANT TRANSPORT; POLYMERASE CHAIN REACTION; WORKPLACE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CONTAMINATION; BACTERIUM ISOLATION; BIOSAFETY; CONTROLLED STUDY; HUMAN; LABORATORY; LABORATORY PERSONNEL; MULTILOCUS SEQUENCE TYPING; NONHUMAN; NOSE SMEAR; RANDOM AMPLIFIED POLYMORPHIC DNA; STAPHYLOCOCCUS AUREUS; WORK ENVIRONMENT;

EQUUS ASINUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 77956919814     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04749.x     Document Type: Article

References (35)

1. van den Akker, E.L., Nouwen, J.L., Melles, D.C., Van Rossum, E.F., Koper, J.W., Uitterlinden, A.G., Hofman, A., Verbrugh, H.A. et al. (2006) Staphylococcus aureus nasal carriage is associated with glucocorticoid receptor gene polymorphisms. J Infect Dis, 194, 814 818. Epub 2006 August 8.
2. Bassetti, S., Bischoff, W.E., Walter, M., Bassetti-Wyss, B.A., Mason, L., Reboussin, B.A., D'agostino, R.B. Jr., Gwaltney, J.M. Jr. et al. (2005) Dispersal of Staphylococcus aureus into the air associated with a rhinovirus infection. Infect Control Hosp Epidemiol, 26, 196 203.
3. van Belkum, A., Kluytmans, J., Van Leeuwen, W., Bax, R., Quint, W., Peters, E., Fluit, A., Vandenbroucke-Grauls, C. et al., et al. (1995) Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains. J Clin Microbiol, 33, 1537 1547.
4. van Belkum, A., Emonts, M., Wertheim, H., De Jongh, C., Nouwen, J., Bartels, H., Cole, A., Cole, A. et al. (2007) The role of human innate immune factors in nasal colonization by Staphylococcus aureus. Microbes Infect, 9, 1471 1477. Epub 2007 August 10.
5. Bischoff, W.E., Wallis, M.L., Tucker, K.B., Reboussin, B.A. Sherertz, R.J. (2004) Staphylococcus aureus nasal carriage in a student community: prevalence, clonal relationships, and risk factors. Infect Control Hosp Epidemiol, 25, 485 491.
6. Bischoff, W.E., Wallis, M.L., Tucker, B.K., Reboussin, B.A., Pfaller, M.A., Hayden, F.G. Sherertz, R.J. (2006) "Gesundheit" sneezing, common colds, allergies, and Staphylococcus aureus dispersion. J Infect Dis, 194, 1119 1126. Epub 2006 Sepetember 11.
7. Boyce, J.M., Potter-Bynoe, G., Chenevert, C. King, T. (1997) Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications. Infect Control Hosp Epidemiol, 18, 622 627.
8. Chambers, H.F. (2001) The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis, 7, 178 182.
9. Choi, C.S., Yin, C.S., Bakar, A.A., Sakewi, Z., Naing, N.N., Jamal, F. Othman, N. (2006) Nasal carriage of Staphylococcus aureus among healthy adults. J Microbiol Immunol Infect, 39, 458 464.
10. Claydon, M.A., Davey, S.N., Edwards-Jones, V. Gordon, D.B. (1996) The rapid identification of intact microorganisms using mass spectrometry. Nat Biotechnol, 14, 1584 1586.
11. Cole, A.M., Tahk, S., Oren, A., Yoshioka, D., Kim, Y.H., Park, A. Ganz, T. (2001) Determinants of Staphylococcus aureus nasal carriage. Clin Diagn Lab Immunol, 8, 1064 1069.
12. Edwards-Jones, V., Claydon, M.A., Evason, D.J., Walker, J., Fox, A.J. Gordon, D.B. (2000) Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry. J Med Microbiol, 49, 295 300.
13. von Eiff, C., Becker, K., Machka, K., Stammer, H. Peters, G. (2001) Nasal carriage as a source of Staphylococcus aureus bacteremia. Study Group. N Engl J Med, 344, 11 16. (Pubitemid 32064839)
14. Enright, M.C., Day, N.P., Davies, C.E., Peacock, S.J. Spratt, B.G. (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol, 38, 1008 1015.
15. Feil, E.J., Cooper, J.E., Grundmann, H., Robinson, D.A., Enright, M.C., Berendt, T., Peacock, S.J., Smith, J.M. et al. (2003) How clonal is Staphylococcus aureus? J Bacteriol, 185, 3307 3316.
16. French, G.L., Otter, J.A., Shannon, K.P., Adams, N.M., Watling, D. Parks, M.J. (2004) Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination. J Hosp Infect, 57, 31 37.
17. Graham, P., Lin, S. Larson, E. (2006) A U.S. Population-based survey of Staphylococcus aureus colonization. Ann Intern Med, 144, 318 325.
18. Greiner, O., Day, P.J., Bosshard, P.P., Imeri, F., Altwegg, M. Nadal, D. (2001) Quantitative detection of Streptococcus pneumoniae in nasopharyngeal secretions by real-time PCR. J Clin Microbiol, 39, 3129 3134.
19. Kluytmans, J., Van Belkum, A. Verbrugh, H. (1997) Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev, 10, 505 520.
20. Kramer, A., Schwebke, I. Kampf, G. (2006) How long do nosocomial pathogens persist on inanimate surfaces? A systematic review BMC Infect Dis, 6, 130.
21. Kuehnert, M.J., Kruszon-Moran, D., Hill, H.A., Mcquillan, G., Mcallister, S.K., Fosheim, G., Mcdougal, L.K., Chaitram, J. et al. (2006) Prevalence of Staphylococcus aureus nasal colonization in the United States, 2001-2002. J Infect Dis, 193, 172 179. Epub 2005 December 15.
22. Lay, J.O. Jr. (2001) MALDI-TOF mass spectrometry of bacteria. Mass Spectrom Rev, 20, 172 194.
23. van Leeuwen, W., Sijmons, M., Sluijs, J., Verbrugh, H. Van Belkum, A. (1996) On the nature and use of randomly amplified DNA from Staphylococcus aureus. J Clin Microbiol, 34, 2770 2777.
24. Lemmen, S.W., Hafner, H., Zolldann, D., Stanzel, S. Lutticken, R. (2004) Distribution of multi-resistant Gram-negative versus Gram-positive bacteria in the hospital inanimate environment. J Hosp Infect, 56, 191 197.
25. Ludwig, W. Schleifer, K.H. (2000) How quantitative is quantitative PCR with respect to cell counts? Syst Appl Microbiol, 23, 556 562.
26. Mertz, D., Frei, R., Jaussi, B., Tietz, A., Stebler, C., Fluckiger, U. Widmer, A.F. (2007) Throat swabs are necessary to reliably detect carriers of Staphylococcus aureus. Clin Infect Dis, 45, 475 477. Epub 2007 July 5.
27. Nouwen, J., Boelens, H., Van Belkum, A. Verbrugh, H. (2004) Human factor in Staphylococcus aureus nasal carriage. Infect Immun, 72, 6685 6688.
28. Rahn, K., De Grandis, S.A., Clarke, R.C., Mcewen, S.A., Galan, J.E., Ginocchio, C., Curtiss, R. III. Gyles, C.L. (1992) Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes, 6, 271 279.
29. Sakwinska, O., Kuhn, G., Balmelli, C., Francioli, P., Giddey, M., Perreten, V., Riesen, A., Zysset, F. et al. (2009) Genetic diversity and ecological success of Staphylococcus aureus strains colonizing humans. Appl Environ Microbiol, 75, 175 183. Epub 2008 October 31.
30. The Federal Authorities of the Swiss Confederation (1999) Ordinance on the Contained Use of Organisms (Containment Ordinance, CO) of 25. August 1999. SR 814.912.
31. Vandenbergh, M.F., Yzerman, E.P., Van Belkum, A., Boelens, H.A., Sijmons, M. Verbrugh, H.A. (1999) Follow-up of Staphylococcus aureus nasal carriage after 8 years: redefining the persistent carrier state. J Clin Microbiol, 37, 3133 3140.
32. Weidenmaier, C., Kokai-Kun, J.F., Kristian, S.A., Chanturiya, T., Kalbacher, H., Gross, M., Nicholson, G., Neumeister, B. et al. (2004) Role of teichoic acids in Staphylococcus aureus nasal colonization, a major risk factor in nosocomial infections. Nat Med, 10, 243 245. Epub 2004 February 1.
33. Wertheim, H.F., Vos, M.C., Ott, A., Van Belkum, A., Voss, A., Kluytmans, J.A., Van Keulen, P.H., Vandenbroucke-Grauls, C.M. et al. (2004) Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet, 364, 703 705.
34. Wertheim, H.F., Melles, D.C., Vos, M.C., Van Leeuwen, W., Van Belkum, A., Verbrugh, H.A. Nouwen, J.L. (2005) The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis, 5, 751 762.
35. Wertheim, H.F., Van Kleef, M., Vos, M.C., Ott, A., Verbrugh, H.A. Fokkens, W. (2006) Nose picking and nasal carriage of Staphylococcus aureus. Infect Control Hosp Epidemiol, 27, 863 867. Epub 2006 July 20.